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Sugar cane juice and starch fermenting process for producing yeast

A technology of starch fermentation and sugarcane juice, applied in the direction of using microorganisms, fungi, etc., can solve the problems of fermentative sugar loss, increase of yeast production cost, waste water not up to standard discharge, etc., to reduce CODCr and chroma, avoid possible Loss of fermentable sugar, effect of reducing waste water treatment cost

Inactive Publication Date: 2007-06-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the main raw material for yeast production in my country is waste molasses, a by-product of the sugar industry. There are mainly the following problems in the production of yeast using waste molasses: 1. During the sugar production process using sugarcane or beet raw materials, part of the sugar is converted into coke at high temperature Sugar pigments, or react with amino acids to produce Maillard pigments, and phenolic pigments, etc., not only cause the loss of fermentable sugar, but also these molasses pigments can also inhibit the growth of microorganisms such as yeast, greatly reducing the fermentation yield; 2. Sugarcane molasses is used as raw material, and about 10-30m 3 COD Cr Up to 50,000-100,000mg / L, and chroma of 3,000-10,000 fermentation stock solution separation wastewater, and COD Cr 20-40m of washing and separation wastewater up to 10000-20000mg / L 3 , In addition, there are pollutants such as molasses pretreatment waste sediment
It is estimated that the wastewater discharged by a dry yeast factory with an annual output of 10,000 tons is equivalent to the pollution load of domestic sewage discharged from a small city with a population of 120,000, and the wastewater treatment is difficult and expensive, which not only greatly increases the production cost of yeast, but also currently In addition to using the concentration method, there is no other practical and feasible discharge treatment technology up to the standard, and the concentration method not only consumes a lot of energy, but also the equipment is prone to fouling and clogging, and there are still many problems to be solved
As a result, the wastewater discharged by most yeast manufacturers has not reached the standard discharge or even seriously exceeded the standard. The pollution problem has become a bottleneck restricting the sustainable development of yeast production enterprises.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Strain used: Saccharomyces cerevisiae Hansen, batch culture process

[0021] After the sugarcane is squeezed, the sugarcane mixed juice with a sugar content of 14.1% is obtained. After the sugar cane juice is cleaned by the sulfite method, tap water is added to prepare a fermentation culture medium with a sugar content of 2.5%. Take 3 liters of clarified cane juice and add it to a 5 liter bioreactor (New Brunswick Scientific, USA). Sterilize at 115°C for 20 minutes, cool to 35°C, add separately heat-sterilized nitrogen source (urea, ammonium sulfate, liquid ammonia) and phosphorus source (phosphoric acid and potassium dihydrogen phosphate), in which the liquid ammonia is not heated, so that the culture The base nitrogen source and phosphorus source content were 5 g / L and 3 g / L, respectively, and 300 ml of Saccharomyces cerevisiae seed liquid was inserted for yeast culture. The initial aeration rate was 2.0 liters / minute, the stirring rate was 250 rpm, and the pH was co...

Embodiment 2

[0023] Strain used: Candida utilis, batch culture process

[0024] After the sugarcane is squeezed, the sugarcane mixed juice with a sugar content of 13.2% is obtained. After the sugarcane juice is cleaned by the sulfite method, tap water is added to prepare a fermentation medium material with a sugar content of 2.5%. Take 3 liters of clarified cane juice and add it to a 5 liter bioreactor (New Brunswick Scientific, USA). Sterilize at 115°C for 20 minutes, cool to 35°C, add nitrogen source (urea, ammonium sulfate, liquid ammonia) and phosphorus source (phosphoric acid and potassium dihydrogen phosphate) for heat sterilization separately, and the liquid ammonia is not heated to make the culture medium The contents of nitrogen source and phosphorus source were 5 g / L and 3 g / L, respectively, and 150 ml of Candida seed solution was inserted for yeast culture. The initial aeration rate was 2.0 liters / min, the stirring rate was 250 rpm, and the pH was controlled to 4.5 with 10% sul...

Embodiment 3

[0026] Strain used: Saccharomyces carlsbergensis Hansen, batch culture process

[0027] After the sugarcane is squeezed, the sugarcane mixed juice with a sugar content of 14.3% is obtained. After the sugar cane juice is cleaned by the sulfite method, tap water is added to prepare a fermentation medium material with a sugar content of 2.5%. Take 3 liters of clarified cane juice and add it to a 5 liter bioreactor (New Brunswick Scientific, USA). Sterilize at 115°C for 20 minutes, cool to 35°C, add separately heat-sterilized nitrogen source (urea, ammonium sulfate, liquid ammonia) and phosphorus source (phosphoric acid and potassium dihydrogen phosphate), in which the liquid ammonia is not heated, so that the culture The contents of the base nitrogen source and phosphorus source were 5 g / L and 3 g / L, respectively, and 200 ml of beer yeast seed solution was added for yeast culture. The initial aeration rate was 2.0 liters / min, the stirring rate was 250 rpm, and the pH was control...

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PUM

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Abstract

The sugar cane juice and starch fermenting process for producing yeast includes: squeezing sugar cane and clarifying to obtain sugar cane juice containing sugar in 11-17 %, setting the sugar cane juice into fermenter, introducing sterilized nitrogen source and phosphorus source, inoculating yeast seed liquid in 5-20 vol%, ventilating and stirring, and culturing. The process utilizes both sugar cane juice and starch as carbon source and energy source separately, and thus has high cell density, high yeast yield and high yeast quality. In addition, the process has the circulation of partial waste water and less pollutant draining.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for fermenting sugarcane juice and starch to produce yeast. technical background [0002] At present, the main raw material for yeast production in my country is waste molasses, a by-product of the sugar industry. There are mainly the following problems in the production of yeast using waste molasses: 1. During the sugar production process using sugarcane or beet raw materials, part of the sugar is converted into coke at high temperature Sugar pigments, or react with amino acids to produce Maillard pigments, and phenolic pigments, etc., not only cause the loss of fermentable sugar, but also these molasses pigments can also inhibit the growth of microorganisms such as yeast, greatly reducing the fermentation yield; 2. Sugarcane molasses is used as raw material, and about 10-30m 3 COD Cr Up to 50,000-100,000mg / L, and chroma of 3,000-10,000 fermentation stock solution separation wastewater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/22
Inventor 梁世中朱明军邓毛程
Owner SOUTH CHINA UNIV OF TECH
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