Sugar cane juice and starch fermenting process for producing yeast
A technology of starch fermentation and sugarcane juice, applied in the direction of using microorganisms, fungi, etc., can solve the problems of fermentative sugar loss, increase of yeast production cost, waste water not up to standard discharge, etc., to reduce CODCr and chroma, avoid possible Loss of fermentable sugar, effect of reducing waste water treatment cost
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Embodiment 1
[0020] Strain used: Saccharomyces cerevisiae Hansen, batch culture process
[0021] After the sugarcane is squeezed, the sugarcane mixed juice with a sugar content of 14.1% is obtained. After the sugar cane juice is cleaned by the sulfite method, tap water is added to prepare a fermentation culture medium with a sugar content of 2.5%. Take 3 liters of clarified cane juice and add it to a 5 liter bioreactor (New Brunswick Scientific, USA). Sterilize at 115°C for 20 minutes, cool to 35°C, add separately heat-sterilized nitrogen source (urea, ammonium sulfate, liquid ammonia) and phosphorus source (phosphoric acid and potassium dihydrogen phosphate), in which the liquid ammonia is not heated, so that the culture The base nitrogen source and phosphorus source content were 5 g / L and 3 g / L, respectively, and 300 ml of Saccharomyces cerevisiae seed liquid was inserted for yeast culture. The initial aeration rate was 2.0 liters / minute, the stirring rate was 250 rpm, and the pH was co...
Embodiment 2
[0023] Strain used: Candida utilis, batch culture process
[0024] After the sugarcane is squeezed, the sugarcane mixed juice with a sugar content of 13.2% is obtained. After the sugarcane juice is cleaned by the sulfite method, tap water is added to prepare a fermentation medium material with a sugar content of 2.5%. Take 3 liters of clarified cane juice and add it to a 5 liter bioreactor (New Brunswick Scientific, USA). Sterilize at 115°C for 20 minutes, cool to 35°C, add nitrogen source (urea, ammonium sulfate, liquid ammonia) and phosphorus source (phosphoric acid and potassium dihydrogen phosphate) for heat sterilization separately, and the liquid ammonia is not heated to make the culture medium The contents of nitrogen source and phosphorus source were 5 g / L and 3 g / L, respectively, and 150 ml of Candida seed solution was inserted for yeast culture. The initial aeration rate was 2.0 liters / min, the stirring rate was 250 rpm, and the pH was controlled to 4.5 with 10% sul...
Embodiment 3
[0026] Strain used: Saccharomyces carlsbergensis Hansen, batch culture process
[0027] After the sugarcane is squeezed, the sugarcane mixed juice with a sugar content of 14.3% is obtained. After the sugar cane juice is cleaned by the sulfite method, tap water is added to prepare a fermentation medium material with a sugar content of 2.5%. Take 3 liters of clarified cane juice and add it to a 5 liter bioreactor (New Brunswick Scientific, USA). Sterilize at 115°C for 20 minutes, cool to 35°C, add separately heat-sterilized nitrogen source (urea, ammonium sulfate, liquid ammonia) and phosphorus source (phosphoric acid and potassium dihydrogen phosphate), in which the liquid ammonia is not heated, so that the culture The contents of the base nitrogen source and phosphorus source were 5 g / L and 3 g / L, respectively, and 200 ml of beer yeast seed solution was added for yeast culture. The initial aeration rate was 2.0 liters / min, the stirring rate was 250 rpm, and the pH was control...
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