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Use of gadol polysaccharide in preparation of antifreeze agent and its products and preparation method

A rhodiola polysaccharide and antifreeze technology, applied in the field of cryobiology, can solve the problem of low cell survival rate and achieve the effect of improving the survival rate

Inactive Publication Date: 2007-05-16
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve this problem, researchers have made a lot of attempts, but the survival rate of cells after freezing is still low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The extraction process of embodiment 1 Rhodiola rosea polysaccharide:

[0067] Accurately weigh 500 grams of dry Rhodiola rosea stalks, cut into 1×1×2cm 3 Large and small blocks, soak in distilled water for 12 hours, filter and remove the filtrate, put it in a 1000ml beaker, add 500ml of distilled water and cook for 2 hours, during the cooking process, you need to keep turning the block solids, so repeatedly cook 3 times and then filter to remove the solids , collect the filtrate, concentrate it to 200ml with a rotary evaporator, add a certain amount of absolute ethanol to make the ethanol concentration reach 80%, let it stand for 12h, then filter it with gauze, and dry the precipitate at a temperature of 60°C to obtain Hongjing Day crude polysaccharides. Dissolve the crude polysaccharide with an appropriate amount of distilled water, add chloroform: n-butanol (chloroform: n-butanol = 4:1), mix well, shake fully for 30 min, centrifuge for 1 min with an electric centrif...

Embodiment 2

[0069] The preparation method of embodiment 2 biological cell freezing antifreeze

[0070] Weigh 2.5 g of glucose and 1.8 g of sodium citrate with an electronic analytical balance, dissolve them in 50 ml of deionized water, stir them evenly with a magnetic stirrer, and prepare 100 ml of base liquid. Get base liquid 80ml and 20ml egg yolk (fresh egg yolk) and mix, add 100,000 IU penicillin and be mixed with I liquid; Add 3% glycerol in I liquid and promptly be mixed with II liquid; The concentration of Rhodiola rosea polysaccharide is 6g / l, and then the pH is adjusted to 6.21 with a precision pH meter, then filtered and sterilized, cooled to room temperature, and placed in a refrigerator at 2-5°C for use. I and II liquids need to be prepared immediately after use.

Embodiment 3

[0071] The preparation method of embodiment 3 biological cell freezing antifreeze

[0072] Weigh 3g of glucose and 1.4g of sodium citrate with an electronic analytical balance, dissolve them in 50ml of deionized water, stir them evenly with a magnetic stirrer, and prepare 100ml of base solution. Get base liquid 80ml and 20ml egg yolk (fresh egg yolk) and mix, add 100,000 IU penicillin and be mixed with I liquid; Add 2% glycerin in I liquid and promptly be mixed with II liquid; The concentration of Rhodiola rosea polysaccharide is 8g / l, and the pH is adjusted to 6.21 with a precision pH meter, and then filtered and sterilized, cooled to room temperature, and put into a refrigerator at 2-5°C for standby. I and II liquids need to be prepared immediately after use.

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PUM

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Abstract

The invention discloses a new application of gadol polysaccharide to prepare freezing deicing fluid of biological cell, mammal sperm, egg, protecting-body cell, early embryo and organ tissue, which comprises the following steps: allocating the basic liquid through glucose, Tris, sodium citrate and deionized water; making the rate of basic liquid d and yolk at 5-9:1-5; adding penicillin and streptomycin to obtain the liquid I; adding glycerin in the liquid I to produce liquid II; adding gadol polysaccharide in the liquid II to obtain the freezing deicing fluid of biological cell.

Description

technical field [0001] The invention relates to the field of cryobiology, relates to a new product with antifreeze function, in particular to the new application of rhodiola rosea polysaccharide in the preparation of antifreeze, the product and its preparation method. Background technique [0002] Rhodiola is a plant of the genus Rhodiola in the sedum family. It is mostly distributed in the alpine regions of the northern hemisphere, and most of them grow in high mountain areas at an altitude of 3,500 to 5,000 meters. At present, the research on Rhodiola is mainly concentrated in Rhodiola In terms of pharmacology of Tiantian, it has a variety of pharmacological effects: ①It can resist various altitude sickness; ②It has the effects of anti-hypoxia, anti-fatigue, and anti-aging; and treatment of cardiovascular diseases; ④In addition, it also has pharmacological effects such as improving the body's immunity, anti-tumor, anti-radiation, anti-virus, improving memory, preventing an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
Inventor 李青旺江中良胡建宏赵红卫李文烨
Owner NORTHWEST A & F UNIV
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