Method for preparing drug or gene carried stent

A gene and drug-loading technology, which is applied in the field of medical materials, can solve the problem that the collagen coating is easily eluted, and achieve the effects of reducing toxicity and immunogenicity, improving transfection efficiency, and increasing drug loading

Inactive Publication Date: 2007-05-16
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem at present is that the collagen coating is easily washed off, and the attached genes are also washed out

Method used

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  • Method for preparing drug or gene carried stent

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 A method for preparing a gene-carrying scaffold, consisting of the following steps:

[0035] (1) Pretreatment of the surface of the bracket

[0036] ① Soak the stent in acetone solution for 30 minutes and ultrasonically clean it for 30 minutes, then use isopropanol solution for ultrasonic cleaning for 30 minutes. Put into oven and dry for subsequent use, sputter 20nm chromium and the metal film of sputtering 50nm gold on the support after drying;

[0037] ② Fixing polymer: Soak the bare gold stent in 0.1M 11-mercaptoundecanol (dissolve 11-mercaptoundecanol in ethanol first, then water, dissolve in 80:20 ethanol and water) for 30 minutes , to form a single-layer self-assembled film on the gold film, rinse with distilled water; then put into 0.6M epichlorohydrin (dissolved in a 1:1 mixed solution of diglyme and 0.4M NaOH) to react 30 minutes, then rinse with water, ethanol, and water once; take it out and then put it into a 0.3g / ml dextran aqueous solution ...

Embodiment 2

[0046] Embodiment 2 A method for preparing a gene-carrying scaffold, consisting of the following steps:

[0047] (1) with embodiment 1;

[0048] (2) Solidification of gene molecules on the surface of the scaffold

[0049] a. Scaffold surface activation: put the fixed polymer scaffold into a mixed aqueous solution of 0.01M N-hydroxysuccinimide and 0.01M ethyl-dimethylaminopropyl-carbodiimide for 1 minute to Activate the scaffold surface;

[0050] b. Immobilization: put the surface-activated stent into a 1000g / L chitosan (carrier) aqueous solution with a pH of 3.6 and soak for 60 minutes, take it out, and put it into a 0.01M ethanolamine hydrochloride aqueous solution with a pH of 9 and soak for 30 minutes. blocking activation group;

[0051] c. Put 0.001g / L gene solution to be loaded and 0.001g / L Lipofectamine TM Soak in the mixed solution of 2000 ℃ for 30 minutes, the gene solution is that the gene is dissolved in the PBS solution that pH is 8 to make, and the Lipofectamin...

Embodiment 3

[0054] Embodiment 3 A method for preparing a gene-carrying scaffold, consisting of the following steps:

[0055] (1) with embodiment 1;

[0056] (2) Solidification of gene molecules on the surface of the scaffold

[0057] a. Scaffold surface activation: soak the fixed polymer scaffold in a mixed aqueous solution of 10M N-hydroxysuccinimide and 10M ethyl-dimethylaminopropyl-carbodiimide for 60 minutes to activate the scaffold surface;

[0058] b. Immobilization: put the surface-activated stent into a 0.1g / L chitosan (carrier) aqueous solution with a pH of 9 and soak for 5 minutes, take it out, and put it into a 10M ethanolamine hydrochloride aqueous solution with a pH of 8 and soak for 1 minute. blocking activation group;

[0059] c. Put 1g / L gene solution to be loaded and 1g / L Lipofectamine TM Soak for 120 minutes in the mixed solution of 2000, and described gene solution is that gene is dissolved in the PBS solution that pH is 5 to make, and described Lipofectamine TM 20...

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Abstract

The invention relates to a method for preparing drug carrier or gene carrier support, wherein said method comprises (1), support surface pretreatment that splashing or plating gold, fixing macromolecule; (2), solidifying the drug or gene molecule on the surface of support that activating the support surface, loading carrier, sealing and solidifying the drug or gene. The invention uses laminated installment, connects mesh macromolecule skeleton on the support, to improve the drug carrier, reduce the toxicity of support and connecting molecule and the immunogen. The invention uses mesh molecule to connect carbohydrate, peptide, and antibody, to connect the drug and antibody firmly and release drug slowly. The invention uses mesh molecule static adsorption gene molecule and transfer medium molecule to improve the transfer efficiency of gene molecule.

Description

technical field [0001] The invention belongs to the field of medical materials, in particular to a blood vessel stent. Background technique [0002] Coronary artery embolism is one of the major diseases that endanger human health. Interventional therapy (PTCA) and bypass surgery are the main clinical treatment methods. Coronary artery stents in interventional therapy play an important role in the treatment of coronary artery embolism. Placement of stainless steel stents after interventional therapy can prevent the collapse of the expanded vessel wall and maintain smooth blood flow, but foreign body stimulation of the stents often leads to excessive growth of local endothelial cells, intimal hyperplasia, and finally re-embolization, which is clinically called restenosis ( RS), the incidence rate of postoperative vascular restenosis is as high as 30-50%, which seriously limits the long-term effect and wide application of treatment, and becomes a difficult problem to be solved ...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/10A61F2/82
Inventor 宋存先鲍军波欧惠超
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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