Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of RhoC guanosine triphosphate

A technology of guanosine triphosphate and liver cancer, which is applied in the application field of detecting the susceptibility of liver cancer, can solve the problems of low abnormal expression rate, unexplained pathogenesis of liver cancer, and no obvious improvement in the survival rate of liver cancer patients

Inactive Publication Date: 2007-04-25
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, the abnormal expression of no less than 20 genes has been determined to be related to the occurrence and development of liver cancer, but the abnormal expression rate of the identified liver cancer-related genes in liver cancer is not high, and the pathogenesis of liver cancer has not yet been elucidated. The early diagnosis rate of
In addition, traditional liver cancer surgery plus chemotherapy and multiple gene therapy methods used in combination in recent years have not significantly improved the survival rate of liver cancer patients. Therefore, finding new liver cancer-related genes, especially genes highly expressed in liver cancer, is of great importance for exploring the pathogenesis of liver cancer. significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of RhoC guanosine triphosphate
  • Application of RhoC guanosine triphosphate
  • Application of RhoC guanosine triphosphate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 , Preparation of protein samples from hepatocellular carcinoma tissue and paracancerous tissue

[0026] Urea, 3-[(3-cholamidopropyl)-diethylammonium]-1-propanesulfonic acid (CHAPS), phenylmethylsulfonyl fluoride (PMSF), dithiothreitol used in this example (DTT) were purchased from Sigma Company.

[0027] In this example, protein samples of cancer tissue and paracancerous tissue of hepatocellular carcinoma were prepared by nonenzymatic sample preparation (NESP), as follows:

[0028] The surgically excised fresh tissue block is quickly placed on ice and quickly cut into several small pieces that are visible to the naked eye without areas of necrosis. Use pre-cooled glutamine-free RPMI1640 medium (5% fetal bovine serum, 0.2mM PMSF, 1mM EDTA, benzisoxazole penicillin 25mg / mL, gentamicin 50mg / mL, penicillin 100U / mL, Streptomycin 100mg / mL, Amphotericin B0.25mg / mL, Nystatin 50U / mL) After washing the small pieces of tissue several times, they were quickly ground in...

Embodiment 2

[0033] Example 2 , Screening of differentially expressed proteins

[0034] Urea, 3-[(3-cholamidopropyl)-diethylammonium]-1-propanesulfonic acid (CHAPS), sodium dodecylsulfonate (SDS), dithiothreitol used in this example (DTT) was purchased from Sigma; iodoacetamide (IAA), acrylamide, N, N-methylenebisacrylamide, etc. were purchased from Fluka; cleavable ICAT reagents were purchased from Applied Biosystems Framingham, MA.

[0035] Ammonium persulfate (AP), TEMED, Tri-n-butylphosphat (TBP), PDQuest software, etc. are Bio-Rad products.

[0036] Avidin affinity column was purchased from Applied Biosystems, Framingham, MA.

[0037] LCQ TM Deca XP system and ProteomeX TM Workstation was purchased from Thermo Finnigan Company.

[0038] The composition of the loading buffer used is: 0.6ml of 1mol / L Tris-HCl (pH6.8), 5ml of 50% glycerol, 2ml of 10% SDS, 0.5ml of mercaptoethanol, 1.9ml of distilled water, and a small amount of hexabromophenol blue.

[0039] First, the gel-enhan...

Embodiment 3

[0047] Example 3 , RhoC GTPase Differential Expression Verification by Western Blot

[0048] In order to confirm the differential expression of RhoC GTPase, 10 hepatocellular carcinoma patients' cancer tissues and corresponding paracancerous tissue protein samples (prepared by NESP method, 10 cases except 429 cases in Table 1) were collected, and purchased Anti-RhoC GTPase antibody was used for western blot analysis, the specific process is briefly described as follows:

[0049] Take 20 μg of protein samples from each sample and use 12% SDS-PAGE to separate, transfer to PVDF membrane (purchased from Amersham Biosciences Company), the primary antibody uses mouse anti-human RhoC GTPase monoclonal antibody (purchased from USBiological Company, 1: 500 dilution), incubated at room temperature for 2 hours, washed three times with TBST (containing 2.42g Tris, 8g sodium chloride, 20ml Tween per liter, adjusted pH to 7.6 with HCl), 5 minutes each time, and the secondary antibody was ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention sifts the protein with differential expression which is in cancer organises of the hepatocyte cancer and nearby cancer organises to obtain a high expression protein in the cancer organizes of the hepatocyte cancer. The differential expression of the RhoC guanosine triphosphatase between the cancers organises of the hepatocyte cancer and nearby cancer organizes exists indeed is confirmed by the immune imprint experiment. In consideration of the correlation between the RhoC guanosine triphosphatase and the hepatoma, the protein can be used as theprotein molecule mark to detect the hepatocyte cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to the application of a RhoC guanosine triphosphate enzyme as an indicator for detecting the susceptibility of liver cancer. Background technique [0002] Liver cancer is a disease that seriously endangers human beings. The incidence of liver cancer in western developed countries is low, and the basic research on liver cancer in the world is still relatively weak. However, my country is a country with a high incidence of liver cancer, and the morbidity and mortality are on the rise, and the age of onset is younger. Medical expenses have increased greatly, and liver cancer has become the number one enemy that seriously endangers the safety of people's life and property in our country, and is an important factor affecting social and economic development. It is of strategic significance to intensify the basic research of liver cancer in my country, and to isolate and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577
Inventor 曾嵘周晓李辰袁新雨
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com