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Process of sugar bio-chip

A biochip and coupling agent technology, applied in biochemical equipment and methods, microbial measurement/inspection, biological testing, etc., can solve the problems of poor accuracy of results, complicated preparation methods, easy falling off of reducing sugars, etc., and achieve low cost , simple connection method, and easy to promote on a large scale

Active Publication Date: 2011-09-14
SHANXI LIFEGEN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing a sugar biochip, which solves the technical problems of the background technology that the reducing sugar is easy to fall off, the accuracy of the result is poor, the preparation method is complicated, and the cost is high.

Method used

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  • Process of sugar bio-chip
  • Process of sugar bio-chip
  • Process of sugar bio-chip

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Embodiment Construction

[0041] The present invention mainly uses reducing sugar as a model, and covalently couples the reducing end of the reducing sugar, that is, hemiacetal (ROH) to the hydroxyl (-OH) derivatized solid phase carrier in the form of glycosidic bonds to prepare the sugar chip.

[0042] The principle of method 1 of the present invention is that the solid-phase carrier derivatized with oxirane groups can be covalently coupled with the amino group of the coupling agent because the surface contains a layer of oxirane groups. The hydroxyl group (-OH) at the other end of the coupling agent undergoes an aldol condensation reaction with the reducing end of the reducing sugar, that is, the 1-position aldehyde group to form a glycosidic bond, so that the reducing sugar is covalently coupled on the solid phase carrier to prepare the sugar chip.

[0043] In this method, the reducing sugar can be monosaccharide, oligosaccharide or polysaccharide. The solid phase carrier can be glass slide, nitroc...

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Abstract

A Method for preparation of sugar biological chip is disclosed that the epoxyethane radical derived solid carrier is covalence coupled with the amidol of coupling agents by the epoxyethane radical contained on the surface, the aldolisation condensation reaction occurred between the hydroxyl which is in the other side of the coupling agents and the deacidizing end of the deacidizing sugar to form the glycosidic bond, the deacidizing sugar is coupled by covalent formula to the solid carrier to form the sugar chip. Or the amidol radical derived solid carrier is covalence coupled with the acid group which is activated by the N-hydroxy succinimide and the dicyclo hexylcar bodiimide of the coupling agents by the amidol radical contained on the surface, the aldolisation condensation reaction occurred between the hydroxy which is in the other side of the coupling agents and the deacidizing end of the deacidizing sugar to form the glycosidic bond, the deacidizing sugar is coupled by covalent formula to the solid carrier to form the sugar chip. The invention resolves the technical matters of that the deacidizing sugar is friable, the Method for preparation is complex, and the cost is high. The invention is simple and quick; the bioactivity of the sugar chip is stable, and easy to apply dimensionally.

Description

technical field [0001] The invention relates to a preparation method of a glycobiological chip. In particular, it relates to a method for preparing a sugar biochip covalently coupled to a hydroxyl-derivatized solid-phase carrier by using a reducing end of a reducing sugar in the form of a glycosidic bond. Background technique [0002] Carbohydrate-binding proteins have important biological functions, which are manifested in the communication and recognition between cells, the interaction between cells and pathogenic microorganisms, the transport of intracellular proteins, the interaction between proteins, the specific binding of proteins and receptors, etc. . The three exploration directions of biochip technology are gene chip, protein biochip and sugar biochip. The advantages of the sugar biochip are: First, the biological activity is stable. Glycobiological chips can preserve biological activity for a long time at room temperature. Second, it has a wide range of applic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48C12Q1/00
Inventor 李铮陈超南刚颜桦
Owner SHANXI LIFEGEN
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