Technique for preparing sponge produced from collagen

Abstract

A process for preparing the collagen sponge includes such steps as extracting collagen in acetic acid by digestion of pepsinum, filtering, taking supernatant, neutralizing by sodium hydroxide, salting out in sodium chloride solution, centrifugal separation of deposit, dissolving it in acetic acid, dialyzing by acetic acid and then by the solution of bisodium hydrogen phosphate, and freeze drying.

Description

technical field [0001] The invention relates to a preparation process of a collagen sponge, in particular to a purified freeze-dried collagen sponge, which can be used for hemostasis and repair of various burns, wounds and surgical wounds, as well as filling of ulcers and residual cavities. Background technique [0002] Collagen is a large family composed of a variety of glycoprotein molecules. It is the main protein component of connective tissue, accounting for about 25% of the total protein in the body. Collagen is a structural protein of the extracellular matrix in which collagen molecules aggregate into supramolecular structures. Collagen is a family of proteins. More than 16 different types of collagen have been discovered. Type I, type II, type III and type IV collagen are discovered through primary structure analysis at the protein level, and others are Discovered by similarity analysis of collagen domains at the nucleic acid level. Collagen, like other proteins, i...

AI Technical Summary

Problems solved by technology

The collagen produced by this process has low protein content and high salt content, and due to the large amount of collagen precipitated after dialysis, the freeze-dried product has poor appearance, hard texture and poor adhesion to the wound surface, and is easy to fall off from the wound surface affect the hemostatic effect