Extractive of Abalone mushroom, extraction method and application thereof

An extraction method and extract technology, which can be applied in the direction of drug combination, anti-toxic agent, freeze-dried delivery, etc., can solve the problems that have not yet seen the extract of oyster mushroom, etc., and achieve the effects of improving the body's immunity, rich resources, and delaying aging

Inactive Publication Date: 2007-02-14
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is mainly used as a kind of edible mushroom in China, and there are no reports on the extract of abalone mushroom as an antioxidant in the body and the extraction process at home and abroad.

Method used

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  • Extractive of Abalone mushroom, extraction method and application thereof
  • Extractive of Abalone mushroom, extraction method and application thereof
  • Extractive of Abalone mushroom, extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation and purification of abalone mushroom fruiting body extract

[0027] 1) Mince 150g of dried abalone mushroom fruiting bodies, soak in 500ml of water, extract under reflux for 2-4 hours, pour out the extract, cool to room temperature and centrifuge, take the supernatant and freeze-dry at -40°C to obtain dry powder.

[0028] 2) Dissolve 2g of dry powder in 50ml of double-distilled water, then add absolute ethanol to an ethanol solution with a final concentration of 80%, centrifuge overnight at 4°C, discard the supernatant, and freeze the precipitate at -40°C with a vacuum freeze dryer Dry to obtain dry powder (P).

[0029] 3) Take 50 mg of the prepared dry powder and dissolve it in 5 ml Tris-HCl buffer solution (10 mM, pH 8.0), load it on an anion exchange cellulose chromatography column (DEAE), and then use 200 ml of 10 mM Tris-HCl, 0.1 N NaCl, 0.5NNaCl, 1N NaCl were eluted sequentially, and the elution rate was to collect 2ml every 3 minutes, freeze-dry a...

Embodiment 2

[0072] Select male SAM mice (provided by Tianjin Disease Prevention Center) and 8-month-old as experimental animals to group, 6 in the administration group, 6 in the control group, and the fruiting body extract of abalone mushroom (P 0.5 ) was added with water to prepare an appropriate aqueous solution, administered by intragastric administration for 30 days, the dosage was 80 mg / kg·bw, and the control group was given an equal dose of normal saline, once a day. After 30 days of administration, the experimental mice were killed by neck dislocation, and the liver tissue was taken to make 10% liver homogenate. After centrifugation, 1ml of the supernatant was taken, and 10μl of absolute ethanol was added. 100 110μl, ice bath for 5 minutes. Dilute the sample 200 times and add to the reaction system, add 2ml of enzyme solution to the assay tube, mix with 1ml of 10mM PBS, measure the change of absorbance at 240nm for 30 seconds, and calculate the CAT enzyme activity.

[0073] The av...

Embodiment 3

[0075] Mouse Lifespan Experiment

[0076] Select 10 SAM mice, all of which are male, and the relevant data record that the average lifespan of SAM mice is 333 days. The test mice are divided into two groups, 5 in the administration group and 5 in the control group. The administration concentration is 30 mg / kg bw. Take orally once daily. The administration time starts from the age of 10 months (after 270 days) until the mice die naturally, see Table 4.

[0077] group

[0078] The above results show that SAM mice were used as animal experiments, and the life span of the mice could be extended from an average of 341 days in the control group to an average lifespan of 394 days by taking the fruiting body extract of abalone mushroom orally after the mice were 9 months old. The control group lived an average of 57 days longer.

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Abstract

An extract of the abalone mushroom for improving immunity, preventing diseases and taking care of human health is composed of the extract P0.5a containing polyose (82.64%) and protein (0.63%) and the extract P0.5b-1 containing polyose (45.48%) and protein (12.63%). Its extracting method is also disclosed.

Description

technical field [0001] The invention relates to the extraction of natural mushroom antioxidant active ingredients, in particular to an extract of oyster mushroom, an extraction method and its application. The extract has natural anti-oxidation activity, can be made into an antioxidant, is used for preventing and treating diseases, and can be used as a health care product for improving body immunity and prolonging life. Background technique [0002] Judging from the current research results, oxygen free radicals are not only related to aging, individual development and cell differentiation, but also to most common major human diseases, from cardiovascular and cerebrovascular diseases with the highest human mortality rate to the most terrible killer of human beings— Cancer and AIDS, as well as neurodegenerative diseases, the biggest challenge facing society with an aging population, are all closely related to free radicals. The purpose of disease prevention and treatment can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/06A61K9/19A61P39/06
Inventor 刘方李乐傅明江荺王春玲宋敏袁芳
Owner NANKAI UNIV
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