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Chemical light emitting method and its device for high snesitivity detecting micro albumin

A microalbumin and chemiluminescence technology, applied in the field of detection of microalbumin, can solve the problems of expensive instruments, high detection cost, complicated operation, etc., and achieve the effect of low cost, high sensitivity and improved luminous intensity

Inactive Publication Date: 2010-12-29
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have relatively high sensitivity, the operation is relatively complicated, the instruments used are expensive, and the detection cost is high.

Method used

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  • Chemical light emitting method and its device for high snesitivity detecting micro albumin
  • Chemical light emitting method and its device for high snesitivity detecting micro albumin
  • Chemical light emitting method and its device for high snesitivity detecting micro albumin

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Experimental program
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Embodiment

[0034] figure 1 Show the specific structure of the device of the present invention, by figure 1 It can be seen that the chemiluminescent device for detecting trace albumin with high sensitivity includes: a darkroom 1, a transparent ultra-thin sample cell 2, a micro sample injector 3, an integrating sphere 4, a chemiluminescence receiving component, an electric pulse processing component, a computer 10, a power supply 11, The voltage stabilizer 12 of the photomultiplier tube; the transparent ultra-thin sample cell 2, the micro sample injector 3, the integrating sphere 4, and the chemiluminescence receiving assembly are arranged in the darkroom, and the chemiluminescence receiving assembly and the electric pulse processing assembly are sequentially connected with the computer 10 connection; the micro-injector 3 is suspended on the sample pool 2; the sample pool 2 is fixed on the wall of the integrating sphere 4; The chemiluminescence receiving assembly includes a lens 5, a phot...

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Abstract

The method includes steps: chemical reaction produces stable single oxygen source; reaction between single state oxygen and high selectivity, high sensitive chemiluminescence probe produces intermediate product in high energy, and sending out photons caused by deexcitation quickly; albumin in minute quantities combined with FCLA enhances sensitivity of chemiluminescence intensity; it is linear relation between magnitude of chemiluminescence enhanced sensitivity and concentration of albumin; using change of luminous intensity corresponding to change of concentration of albumin can detect albumin in trace amount. Equipment for implementing the method includes darkroom, sample cell, sample injector in minute quantities, integrating sphere, module for receiving chemiluminescence, module for treating electrical pulse, and computer etc. Features are: small sample needed, short time consumption, low cost, high sensitivity, and certain degree of interference immunity.

Description

technical field [0001] The invention relates to a technology for detecting microalbumin, in particular to a chemiluminescence method and a device for detecting microalbumin with high sensitivity. Background technique [0002] Serum albumin (HSA) is the most abundant protein in serum, accounting for about 40% to 60% of the total serum protein. It plays an important role in the transportation of drugs and maintaining normal blood osmotic pressure. [0003] At present, the commonly used methods for analyzing albumin are: (1) dry test strip detection method; using the protein error principle of the indicator (that is, the indicator ion is combined with the opposite charge of the albumin, so that it reacts with the displayed pH color Change to a higher pH color change, the magnitude of this pH color change is proportional to the albumin content) established. (2) Heating acetic acid method: heating and boiling to denature and coagulate the protein, and then adding acid to make t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N21/76
Inventor 邢达徐未周静
Owner SOUTH CHINA NORMAL UNIVERSITY
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