Active protein feed and its preparing method
An active protein and feed technology, applied in the field of feed, can solve the problems of increasing production costs, increasing labor intensity, and being unfavorable for the reuse of distiller's grains, and achieves the effects of promoting digestion and absorption, reducing labor intensity, and saving energy and food resources.
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Embodiment 1
[0033] One, prepare Aspergillus niger Aspergillus aspergillus strain, concrete method is as follows:
[0034] 1. Preparation of test tube strains (Aspergillus niger strain: CGMCC 3.0040, purchased from the General Microorganism Strain Collection Center, Institute of Microbiology, Chinese Academy of Sciences):
[0035] (1) Sterilization of test tubes: clean the test tubes, drain the water, dry them in an oven, put cotton plugs on them, and sterilize them by dry heat at 140°C for 120 minutes.
[0036] (2) Preparation of slant Cha's culture medium
[0037] ① Cha's medium formula: NaNO 3 3g, K 2 HPO 4 ·3H 2 O 1g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2O 0.01g, KCl 0.5g, agar 20g, water 1000ml.
[0038] ②Preparation: Put the above ingredients in an aluminum pot, heat to dissolve and cool slightly, divide into test tubes, plug them with cotton plugs, sterilize at 0.1MPa for 30 minutes, take them out and place them on an inclined plane, and then culture them at a constant tempera...
Embodiment 2
[0068] One, prepare the aspergillus oryzae koji dish bacterial classification, concrete method is as follows:
[0069] 1. Preparation of test tube strains (Aspergillus oryzae strain: CGMCC3.0320, purchased from the General Microorganism Strain Collection Center, Institute of Microbiology, Chinese Academy of Sciences):
[0070] (1) Sterilization of test tubes: clean the test tubes, drain the water, dry them in an oven, put cotton plugs on them, and sterilize them by dry heat at 140°C for 120 minutes.
[0071] (2) Preparation of slant Cha's culture medium
[0072] ① Cha's medium formula: NaNO 3 3g, K 2 HPO 4 ·3H 2 O 1g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, KCl 0.5g, agar 20g, water 1000ml.
[0073] ②Preparation: Put the above ingredients in an aluminum pot, heat to dissolve and cool slightly, divide into test tubes, plug them with cotton plugs, sterilize at 0.1MPa for 30 minutes, take them out and place them on an inclined plane, and then culture them at a consta...
Embodiment 3
[0103] One, prepare Candida utilis Aspergillus strain, specific method is as follows:
[0104] 1. The preparation of Candida utilis strains in test tubes, flask strains and Karst jar strains is usually carried out by conventional distiller's culture. See "Raw Material Wine Brewing Technology" published by China Light Industry Publishing House, edited by Huang Ping, deputy director Liu Yigang and Yu Minghua Editor-in-Chief (Candida utilis strain: purchased from the General Microorganism Culture Collection Management Center of Guangdong Institute of Microbiology, strain number: GIM2.148).
[0105] (1) Cultivation of Candida utilinus solid yeast seeds:
[0106] ①Ingredients: 50% bran, 50% fresh distiller's grains, 80% water added to the bran, mix evenly and sieve, no sandwich and no lumps are required.
[0107] ②Steaming ingredients: Cook at normal pressure for 1 hour, take out the retort and let it cool to about 30°C.
[0108] ③Inoculation: Inject the activated 50,000 units / g ...
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