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Bulbous flower tissue culture seedling cultivating process to form bulb inside container

A cultivation method and a technology of tissue culture seedlings, which are applied in the field of cultivation of bulbous flower tissue culture seedlings into balls in containers, can solve the problems of low survival rate of tissue culture seedlings transfer, disease infection, etc., to save production space and time, improve The effect of ball speed and avoiding unfavorable environmental conditions

Inactive Publication Date: 2007-01-03
甘肃省花卉工程技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the rapid propagation of tissue culture, problems such as low survival rate of tissue culture seedlings and disease infection often occur

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The specific steps are as follows:

[0013] 1) Preparation of the incubator: with CO 2 The incubator of the forced air supply system is used as the culture container; the culture container is made of organic glass, the volume is 120L, the volume is 120-130L, and the culture area is 5180cm 2 , Placed on a culture rack for multi-layer three-dimensional culture; 8 40W fluorescent lamps are installed on the top of the box, and there is a 5cm gap between the light source and the top of the box; the side of the box has an air inlet pipe and a movable door, and the top of the box There are vent holes in the four corners, and the vent holes are pasted with a bacterial filter; CO 2 Compulsory gas supply system by CO 2 Source, air pump and connecting pipes, which combine pure CO 2 Mixed with air to form CO with a concentration of 1000-1500mg / L 2 After the gas is sterilized and dried, it is forced into the incubator to supply sufficient CO to the plants 2 , To promote photosynthesis o...

Embodiment 2

[0018] The method for cultivating colored calla lily into balls in a container is as follows:

[0019] 1) Preparation of the incubator: with CO 2 The incubator of the forced air supply system is used as the culture container, with a volume of 130L and a culture area of ​​5610cm 2 ; There are 4 40W fluorescent lamps on the top of the box, and there is a gap of 8cm between the light source and the top of the box; the side of the box has an air inlet pipe and a movable door, and the top four corners of the box have air outlets, and the air outlets are pasted with bacteria Membrane; CO 2 Compulsory gas supply system by CO 2 Source, air pump and connecting pipes, which combine pure CO 2 Mixed with air to form CO with a concentration of 1000-1500mg / L 2 After the gas is sterilized and dried, it is forced into the incubator to supply sufficient CO to the plants 2 , To promote photosynthesis of plants; the incubator should be fully disinfected before use;

[0020] 2) Selection of raw mater...

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Abstract

The bulbous flower tissue culture seedling cultivating process for inducing formation of bulb inside a large container with high bulb forming speed includes the following steps: 1. preparing incubator with forced CO2 supplying system as the culture container; 2. selecting healthy secondary proliferous test tube plantlet as the material for inducing bulb; 3. inducing micro seed bulb with the test tube plantlet in no-sugar vermiculite culture medium comprising 1 / 2MS+NAA 0.1-0.3 mg / L+IBA0.-0.4 mg / L+PP333 5.0-10.0 mg / L or 1 / 2MS+IBA 0.2-0.4 mg / L+PP333 5.0-10.0 mg / L; and 4. air drying the collected the micro seed bulb at room temperature, storing in refrigerator, and treating at 4-10 deg.c for 4-8 weeks before planting in blooming bulb culturing land. The present invention is suitable for lily, color calla and other bulbous flower, and has saving in producing space and time.

Description

Technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for culturing bulbous flower tissue culture seedlings into balls in a container. Background technique [0002] The flower industry ranks second among the 10 most promising industries in the 21st century. People's requirements for the quantity and quality of flowers are constantly improving. Bulb flowers such as lilies and colored calla lilies are one of the most important fresh cut flowers in the world, occupying an increasingly important position in the domestic and international flower market. In recent years, the average annual growth rate in my country has exceeded 20%. However, the current technology for breeding bulbs of lily, colored calla lily and other flower bulbs in my country is relatively backward. Good bulbs basically rely on imports, and more than 100 million bulbs need to be imported from abroad every year, which not only costs a lot of foreign exchange, bu...

Claims

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Application Information

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IPC IPC(8): A01G9/00A01G1/00A01H4/00
Inventor 师向东邱仲华
Owner 甘肃省花卉工程技术研究中心
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