Method for identifying 28 frequent phathogenic bacteria for clinical bacteremia

Abstract

This invention relates to a method for identifying 28 kinds of common pathogens of clinical bacteremias, which belongs to the molecular biology and biochemistry field of biotechnology, relates to a suspension chip technology for identifying common pathogens of clinical bacteremias, applies to culture identification for clinically separating the bacterial strain and provides guide for clinical bacteremias patient to accurately and reasonably treating by using antibacterial agents, which crosslinks 30 types of specific probes against the 28 kinds of common pathogens on 30 kinds of different fluorescent microspheres to be reacted with the being tested specimens then to be reacted with the report molecules labeled by fluorescent elements to test the specific 23SrDNA of them on a fluorescent test device. The method comprises advantages of high susceptibility, great specificity, good bacterial strain efficient and short detecting time.

Description

technical field [0001] The invention belongs to the field of molecular biology and biochemistry of biotechnology, relates to a suspension chip technology for identifying common pathogenic bacteria in clinical bacteremia, is suitable for identification of bacterial strains isolated clinically, and accurately and reasonably conducts antibacterial treatment for patients with clinical bacteremia Drug therapy provides guidance. Background technique [0002] Isolation of bacteria from blood cultures indicates a serious infection requiring immediate antimicrobial therapy. Different pathogenic bacteria have different sensitivities to drug types and concentrations, and the key to successful treatment lies in the early application of appropriate and sufficient drugs. It usually takes 1 to 3 days to isolate and identify bacteria in blood culture, during which improper medication often makes the patient's condition worse or increases the drug resistance of the strain. Especially in th...

AI Technical Summary

Problems solved by technology

However, the traditional solid-phase biochip has gradually exposed its shortcomings in the long-term use process: (1) poor flexibility: biochips are prefabricated according to a certain pattern, and cannot adapt to the ever-changing needs of patients; (2) ) detection takes a long time: because the reaction is carried out on glass or membrane substrates, it must be washed repeatedly to remove excess reaction starters, intermediate products and hybridization buffers, etc.; (3) repeatability Not ideal: Hybridization on glass or membrane substrates has many influencing factors, and it is difficult to strictly control the experimental conditions

(4) Expensive: The biochips currently in clinical use are all expensive, which is unbearable for patients

Images