Bailing mushroom new variety, and bacterial species production and culture method
A technology of Bailing mushroom and a new variety, applied in the fields of botany equipment and methods, horticulture, application, etc., can solve problems such as poor uniformity and shape consistency, lower commodity rate and high-quality product rate, and commercial scale production restrictions. , to achieve the effects of beautiful mushroom body shape, increased commodity rate, and improved labor efficiency
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Embodiment 1
[0033] Example 1 Breeding and identification of strains
[0034] The wild fruit bodies were collected from the stem base of Asafoetida in Xinjiang, China. According to morphological observations, the mating test was carried out with the similar species Pleurotus eryngii var.ferula, which also occurs on Asafoetida in Xinjiang, and rDNA-ITS Size and sequence detection, identified as Bailing Mushroom, Chinese name Bailing Pleurotus, Latin name Pleurotus nebrodensis (Inzengae) Quél., the wild fruit body tissue isolate and the culture after the hybridization of polyspores were cultivated and screened, and invented This patent.
[0035] The spores are separated by ejecting spores under low light conditions at room temperature of 20℃~22℃. After collecting the white powder, a part of it is transferred to the PDA plate for polyspore hybridization to obtain a polyspore hybrid culture, and the other part is made with sterile water Spore liquid with appropriate concentration, after spreading ...
Embodiment 2
[0037] Example 2 Strain production method
[0038] 1. Mother species: PDA culture medium, cultivated at 24-28℃, overgrown slant or flat plate for 10-12 days, storage temperature is 4℃.
[0039] 2. Original and cultivated species: cottonseed hull culture medium, the formula is 95% or 90% of cottonseed hull, 4% corn meal or 9% wheat bran, 1% gypsum, water content is 1:1.4, 22~26 Cultivation at ℃, the original species will grow up in the container for 40 to 45 days, and the cultivated species will grow up in about 35 days. The storage temperature is 4°C. The original seed can also use grains as a culture medium, which is made according to the conventional method, and the container will grow in about 20 days.
Embodiment 3
[0040] Example 3 Cultivation method
[0041] 1. Cultivation technology: culture medium production → cooling inoculation → germ growth → post-ripening → low temperature stimulation → mushroom fruiting → harvest.
[0042] 2. Cultivation medium: 90% cotton seed hulls, 6% corn flour, 2% lime, 1% gypsum, 1% potassium dihydrogen phosphate, and a water content of 1:1.5-1.6.
[0043] 3. Bacteria development conditions: room temperature 22~24℃, air relative humidity 60~75%, dark light, good ventilation.
[0044] 4. Post-cooking conditions: room temperature 18-20℃, relative air humidity 60-75%, low light, good ventilation.
[0045] 5. Low temperature stimulation: Place the bacteria bag at an ambient temperature of -5~5℃ for 7 days.
[0046] 6. Fruiting conditions: agricultural cultivation temperature 5~18℃, industrial cultivation 12~15℃, air relative humidity 75~85%, light 200~800 Lux, good ventilation, environment CO 2 ≤400×10 -6 .
[0047]7. Cultivation cycle: Under the above conditions, ...
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