Related gene and protein for preventing and treating Japanese blood fluke infection, and its uses

A serum albumin, gene technology, applied in the fields of biopharmaceuticals, genetic medicines and genetic engineering products

Inactive Publication Date: 2006-07-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no particularly effective preventive method against schistosomiasis japonicum

Method used

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  • Related gene and protein for preventing and treating Japanese blood fluke infection, and its uses
  • Related gene and protein for preventing and treating Japanese blood fluke infection, and its uses
  • Related gene and protein for preventing and treating Japanese blood fluke infection, and its uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0053] 1. Separation and purification of serum albumin in Voles orientalis.

[0054] Adult orientalis voles were anesthetized with ether, and blood was collected from the eye sockets. The blood was collected in glass test tubes and kept overnight at 4°C in the refrigerator to coagulate the blood. After blood coagulation, centrifuge at 3000 rpm for 5 minutes to collect serum.

[0055] Orient vole serum in buffer I (0.05M KH 2 PO 4 , pH 7.0) was diluted 1:1, centrifuged at 10,000 rpm to remove insoluble substances, and loaded on the top of the blue agarose gel FF affinity chromatography column. The column was eluted with buffer I to remove unbound protein. followed by buffer II (0.05M KH 2 PO 4 , 1.5M KCl, pH7.0) to elute the serum albumin, and collect the eluate. The dialysis bag (dialysis molecular weight 8000-14000 kDa) was dialyzed and equilibrated with buffer I for 24 hours (during which the medium was changed at least 3 times). The eluate containing serum albumin w...

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Abstract

The invention discloses a protein sequence for killing Japanese Schistosoma and preventing and treating Japanese Schistosoma infection and the corresponding gene sequence and its usage. The protein sequence uses affinity chromatography, polyacrylamide gel electrophoresis; dialysis, freeze drying and protein amino end test sequence to separating obtain the blood serum albumin from oriental hamster unguent. It uses polyase chain reaction technology to obtain oriental hamster blood serum albumin gene sequence. The gene and the decorated mutant insert into the eukaryon expression holder in the animal body, which can be used in preventing and treating the gene of Japanese Schistosoma infection.

Description

technical field [0001] The invention relates to biopharmaceuticals, gene medicines and genetic engineering products, and further refers to the separation and purification of serum albumin of oriental voles and the development of genetic engineering products. Development of serum albumin and albumin gene product of oriental vole, and its application in the prevention and treatment of schistosomiasis japonicum. Background technique [0002] According to the statistics of the World Health Organization, schistosomiasis is prevalent in more than 70 countries around the world, 200 million people are infected with schistosomiasis, 120 million people have clinical symptoms of infection, and about 20,000 people die of schistosomiasis every year. The schistosome in our country is Schistosoma japonicum. Since the founding of New China, my country has made great achievements in the control of schistosomiasis, but there are still 108 counties (cities, districts) in the country that have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/36C12N15/12C12N15/86C12N15/85C12N15/70C12N15/74A61K48/00A61K38/38
CPCY02A50/30
Inventor 胡维新邬国军秦志强
Owner CENT SOUTH UNIV
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