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Whitening and antioxidative cosmetic composition containing resveratrol and method for preparing the same

A technology of resveratrol and composition, applied in the field of whitening and anti-oxidative cosmetic composition, can solve the problems of low solubility, peculiar smell, discoloration satisfaction, etc.

Inactive Publication Date: 2010-10-13
ENPRANI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it can also cause problems such as excessive use of polyols in cosmetics due to low solubility of α-lipoic acid, unique odor of sulfur elements, and odor and discoloration caused by decomposition of thiol groups under light conditions, resulting in low satisfaction.

Method used

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  • Whitening and antioxidative cosmetic composition containing resveratrol and method for preparing the same
  • Whitening and antioxidative cosmetic composition containing resveratrol and method for preparing the same
  • Whitening and antioxidative cosmetic composition containing resveratrol and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: Tyrosinase inhibitory activity test

[0063] The whitening effect of resveratrol was evaluated by measuring the inhibitory activity of resveratrol on tyrosinase in the main biosynthesis process of melanin, and the results are shown in Table 1.

[0064] Tyrosinase acts as a substrate in the process of melanin synthesis. Mushroom tyrosinase or tyrosinase fragments are isolated from melanocytes, added to test samples, and incubated for a certain period of time. The absorbance of the reaction product dopachrome is tested at one absorption wavelength to evaluate the whitening effect of the test sample.

[0065] Tyrosinase inhibitory activity (%)=100-((ODexp-ODcon) / ODstd×100)

[0066] ODexp: absorbance of test sample containing tyrosinase

[0067] ODcon: Absorbance of test sample without tyrosinase

[0068] ODstd: Absorbance of a standard sample containing tyrosinase

[0069] Table 1: Test results of tyrosinase inhibitory activity

[0070]

[0071] * I...

Embodiment 2

[0074] Embodiment 2: free radical purification test

[0075] In order to evaluate the antioxidant activity of resveratrol, the free radical scavenging test was carried out according to the following method, and the results are shown in Table 2.

[0076] Due to the scavenging effect of the relatively stable dark blue DPPH (1,1-diphenyl-2-hydrazone) free radical, the methanol solution of the test sample becomes clear, and the degree of reduction in the absorbance of the methanol solution is measured at 516 nm.

[0077] Free radical purification (%)=100-((ODexp-ODcon) / ODstd×100)

[0078] ODexp: Absorbance of test sample containing DPPH

[0079] 0Dcon: absorbance of test sample without DPPH

[0080] ODstd: Absorbance of a standard sample containing DPPH

[0081] Table 2: Test results of free radical scavenging activity

[0082]

[0083] * ICso: 50% tyrosinase inhibitor activity concentrate (when Icso decreases, tyrosinase inhibitor activity increases)

[0084] * Whitegenti...

Embodiment 3

[0086] Example 3: Whitening test based on skin melanocyte proliferation

[0087] The inhibitory activity of resveratrol on melanin produced by melanocytes was tested on healthy people, and the results are shown in Table 3.

[0088] The whitening test based on skin melanocyte proliferation is carried out as follows:

[0089] 1. Place human skin melanocytes in MGM (Melanocyte Growth Medium, Clonetics) medium at 37°C, 5% CO 2 cultured in an incubator.

[0090] 2. Predetermined number of cells (2×10 6 ) were cultured in a 75T culture flask. When cell aggregation is found, the test sample at a predetermined concentration is put into the cell culture medium and cultivated for 2-3 days.

[0091] 3. Remove the culture medium, wash with phosphate buffer saline (PBS), and treat with insulin solution to obtain the desired cells.

[0092] 4. The resulting cells are centrifuged into cell pellets. The color of the cell pellets can be observed. Cell pellets were dissolved in 1 N NaOH ...

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Abstract

Provided are a stabilized, skin-whitening and antioxidative cosmetic composition including resveratrol as a main component and either a primary stabilizer selected from cyclodextrin, polyethyleneglycol, and a mixture thereof, or a combination of the primary stabilizer and a secondary stabilizer selected from an alpha-lipoic acid, a water-soluble whitening composition, a phellodendron extract, an alteromonas ferment extract, and a mixture thereof, and a method for preparing the same. The cosmetic composition provides excellent skin whitening and antioxidative effects for a long-term even when used in a small quantity. Furthermore, solubility of the resveratrol and the alpha-lipoic acid in polyol or ethanol is enhanced, thereby improving low use satisfaction and skin irritation that may be caused due to excess use of an organic solvent. In addition, problems such as crystal precipitation at low temperature, off-flavor and discoloration upon exposure to sun light can be solved.

Description

technical field [0001] The invention relates to a whitening and antioxidant cosmetic composition containing resveratrol and a preparation method thereof. More specifically, the present invention relates to a cosmetic composition containing resveratrol as the main ingredient and also a primary stabilizer selected from cyclodextrins, polyethylene glycols and mixtures thereof, or a primary stabilizer with a secondary The stabilizer compound, the secondary stabilizer is selected from the group consisting of alpha-lipoic acid, water-soluble whitening composition, Phellodendron cork extract, Alteromonas enzyme extract and mixture thereof, and a method for preparing the cosmetic composition. Background technique [0002] As an important tissue to protect the internal organs of the human body, the skin affects the biochemical and physical functions of the human body when it is in direct contact with the outside world. It is mainly composed of three layers, namely the epidermis, der...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/34A61Q19/02A61Q19/08
CPCB08B7/0021B08B9/093
Inventor 金晓靖郑成源金赫金锡在李周东柳熙昌权爱英赵美罗
Owner ENPRANI CO LTD
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