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Wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its usage

A technology of powdery mildew resistance gene and disease resistance gene, which is applied in genetic engineering, plant gene improvement, application, etc., can solve the problem of being in a homozygous or heterozygous state that cannot be known, and achieve the effect of tight linkage

Inactive Publication Date: 2006-02-22
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, using SCAR 1400 and SCAR 1265 When performing molecular marker-assisted selection or identifying the purity of seeds, it can only be determined whether the material contains 6VS / 6AL chromosomes, but whether this chromosome is in a homozygous or heterozygous state cannot be known

Method used

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  • Wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its usage
  • Wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its usage
  • Wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its usage

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Experimental program
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Embodiment Construction

[0036] 1. Screening for disease resistance gene analogues located on the 6VS chromosome

[0037] The powdery mildew resistant 6VS / 6AL translocation line was backcrossed with Yangmai 5 for 7 generations, and a translocation line with a background close to that of Yangmai 5 was obtained. The translocation line and the parent Yangmai 5 were cultivated in pots, and used The cover made of transparent film is isolated to prevent external bacteria from polluting. When the plants reached the second-leaf stage, the translocation line was inoculated with the powdery mildew mixed strain by shaking off, and induced for 6 hours and 12 hours respectively, while Yangmai 5 was not induced by the powdery mildew. Then extract the RNA from the leaves of the induced translocation line and the leaves of Yangmai 5 without induction, and construct the forward suppressive subtraction hybridization (SSH) library with the RNA of the translocation line as the Tester and the RNA of Yangmai 5 as the Drive...

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Abstract

The invention discloses a wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its use, wherein disease-resistant gene analogues Ta-LRR2 sequence design primers NAU / XiBao 16F and NAU / XiBao 16R are obtained through suppression subtractive hybridization between Yangmai No.5 and translocation lines, a series of material tests have shown that, the label NAU / XiBao16 is interlocked with translocation lines 6VS / 6AL powdery mildew resistant gene Pm21, and one PCR experiment can amplify two specific belts from common wheat 6AS and Haynaldia Vollosa 6VS chromosomes simultaneously, the label NAU / XiBao16 can be used for the molecular label auxiliary selection in wheat powdery mildew disease resistant breeding, and for the purity identification of common wheat seeds.

Description

1. Technical field [0001] The invention discloses a marker linked with wheat powdery mildew resistance gene Pm21, which can be used for molecular marker-assisted selection in wheat powdery mildew resistance breeding; the marker can also be used to identify the purity of common wheat seeds, especially to identify those created by cell engineering and chromosome engineering Materials involving mutations in chromosome 6AS of homolog group VI of common wheat. 2. Technical background [0002] Wheat (T. aestivum L), as an important food crop worldwide, plays a pivotal role in agricultural production, but its production is affected by many biotic and abiotic stresses. Wheat powdery mildew (Ergsiphe greminis f.sp.tritici), caused by the obligate parasitic fungus Erysiphegraminis DC, is one of the diseases that are increasingly harmful to wheat production in China and many countries in the world. improvement, and there has been an increasing trend in recent years. [0003] Common w...

Claims

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Application Information

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IPC IPC(8): C12N15/65C12N15/29C12Q1/68
Inventor 陈雅平陈佩度王秀娥张守忠王苏玲周波
Owner NANJING AGRICULTURAL UNIVERSITY
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