Reagent for measuring low-density lipoproteincholesterol and preparation method
A low-density lipoprotein and cholesterol technology, which is applied in the field of reagents and preparations for the determination of serum components, can solve the problems of adverse effects of automatic biochemical analyzers, determination of LDL cholesterol, and direct detection, so as to reduce the detection workload and reduce detection time. , the effect of high accuracy
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Embodiment 1
[0061] Example 1: Determination of LDL cholesterol using a reagent prepared from digitonin compound and enzyme-prepared high-affinity enzyme compound and surfactant-nonylphenol polyoxyethylene ether sodium ammonium sulfate
[0062] Reagent I (R1)
[0063] Buffer MES (PH: 7.0) 100mmol / L
[0064] Stabilizer MgCL.6H 2 O 2mmol / L
[0065] Chromogen 4-AAP 0.25mmol / L
[0066] Preservative NaN3 5mmol / L
[0067] Stabilizer BSA 0.5g / L
[0068] Catalyst sodium cholate 20mmol / L
[0069] High affinity cholesterol esterase compound 1500U / L
[0070] High affinity cholesterol oxidase compound 3000U / L
[0071] Anti-interference substance ascorbate oxidase 1000U / L
[0072] Surfactant Polyoxyethylene lauryl ether 13g / L (when reagent I is mixed with reagent II, the content is 10g / L)
[0073] Reagent II (R2)
[0074] Buffer MES (PH: 7.0) 100mmol / L
[0075] Stabilizer BSA 0.5g / L
[0076] Preservative NaN3 5mmol / L
[0077] Stabilizer MgCL.6H2O 2mmol / L
[0078] Catalyst horseradish pero...
Embodiment 2
[0083] Example 2: Determination of LDL cholesterol using a reagent prepared from aescin and enzyme-prepared high-affinity enzyme compound and surfactant-alkyl polyoxyethylene ether hydroxypropyl sulfonate
[0084] Reagent I (R1)
[0085] Buffer MOPS (PH: 6.7) 50mmol / L
[0086] Stabilizer EDTA.2Na 2mmol / L
[0087] Chromogen 4-AAP 0.20mmol / L
[0088] Preservative NaN3 5mmol / L
[0089] Stabilizer BSA 0.5g / L
[0090] Catalyst sodium cholate 15mmol / L
[0091] High affinity cholesterol esterase compound 350U / L
[0092] High affinity cholesterol oxidase compound 3500U / L
[0093] Anti-interference substance ascorbate oxidase 1000U / L
[0094]Surfactant Alkyl polyoxyethylene ether hydroxypropyl sulfonate sodium 30g / L (when reagent I is mixed with reagent II, the content is
[0095] 22.5g / L)
[0096] Reagent II (R2)
[0097] Buffer MOPS buffer (PH: 6.7) 50mmol / L
[0098] Stabilizer BSA 0.5g / L
[0099] Preservative NaN3 5mmol / L
[0100...
Embodiment 3
[0106] Example 3: Determination of LDL cholesterol using a reagent prepared from lipoprotein dehydrated protein, a high-affinity enzyme compound prepared by an enzyme, and a surfactant-polyoxyethylene higher alcohol ether.
[0107] Reagent I (R1)
[0108] Buffer PIPES (PH: 6.7) 200mmol / L
[0109] Stabilizer MgCL.6H2O 2mmol / L
[0110] Chromogen 4-AAP 0.25mmol / L
[0111] Preservative NaN3 5mmol / L
[0112] Stabilizer BSA 0.5g / L
[0113] Catalyst sodium cholate 20mmol / L
[0114] High affinity cholesterol esterase compound 2600U / L
[0115] High affinity cholesterol oxidase compound 260U / L
[0116] Anti-interference substance ascorbate oxidase 1000U / L
[0117] Surfactant Polyoxyethylene higher alcohol ether 66g / L (when reagent I is mixed with reagent II, the content is 50g / L)
[0118] Reagent II (R2)
[0119] Buffer PIPES (PH: 6.7) 200mmol / L
[0120] Stabilizer BSA 0.5g / L
[0121] Preservative NaN3 5mmol / L
[0122] Stabilizer MgCL.6H2O 2mmol / L
[0123] Catalyst horserad...
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