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Technique of detectig endogenic fungus from flinty grass

A technology of endophytic fungi and detection technology, which is applied in the field of detection, can solve the problems of affecting accuracy, insufficient thickness, and difficulty in tearing off the endothelial film, and achieve the effect of improving accuracy, high accuracy rate, and shortening the working process

Inactive Publication Date: 2005-08-31
LANZHOU UNIVERSITY
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  • Summary
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AI Technical Summary

Problems solved by technology

However, this detection method is difficult to tear off the endothelial film of some grasses with relatively hard texture, such as drunk horse grass, lymus (Leymus), wheatgrass (Agropyron), even if the cortex obtained by tearing is often thick Insufficient, thus affecting the detection of such endophytic fungi by leaf sheath staining and light microscopy, and also affecting the accuracy of detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] Materials: Drunken horse grass samples collected from Gansu, Qinghai, Xinjiang and Inner Mongolia, the seeds with aleurone layer staining and detection to find the bacteria-carrying rate of 100%, were planted in 16×6-eye plastic breeding trays, at 15- It was grown in a greenhouse at 30°C, and a single seedling within 4 weeks of growth was taken as a sample.

[0016] Method: Soak the tillers of early-age seedlings in 5-15% NaOH aqueous solution at room temperature for 3-5 hours, then place them directly on glass slides, and then stain them with 0.8% lactate aniline blue solution. Pressure, and then heated with an electric heating plate or alcohol flame for a few seconds, then detected under an optical microscope.

[0017] Results: After the leaf sheaths of 2000 early-age seedlings of Drunken Horseweed were treated by the present invention, the detection rate of endophytic fungi was 98%. However, using the prior art, the samples are extremely difficult to handle, and the...

example 2

[0019] Materials: Drunken horse grass samples collected from Gansu, Qinghai, Xinjiang and Inner Mongolia, the seeds with aleurone layer staining and detection to find the bacteria-carrying rate of 100%, were planted in 16×6-eye plastic breeding trays, at 15- Grow in a greenhouse at 30°C, and take old young plants at the age of 5 weeks.

[0020] Method: Boil the leaf sheaths of young young plants in boiling water for 4 to 6 minutes, then tear off the inner cortex along the inner side of the leaf sheath, place the torn endothelial layer on a glass slide, and then stain with 0.8% lactic acid aniline blue solution, and then use After being heated by a hot plate or an alcohol flame for a few seconds, it is detected under an optical microscope.

[0021] Result: After 2000 mature leaf sheaths of Drunken Horseweed were treated by the present invention, the detection rate of endophytic fungi was 100%. However, using the existing technology, the samples are very difficult to handle, an...

example 3

[0023] Materials: Drunken horse grass samples collected from Gansu, Qinghai, Xinjiang and Inner Mongolia, the seeds with aleurone layer staining and detection to find the bacteria-carrying rate of 100%, were planted in 16×6-eye plastic breeding trays, at 15- Grow in a greenhouse at 30°C, and take adult plants at the age of 15 months.

[0024] Method: Boil the adult plant leaf sheath with boiling water for 5-8 minutes, then tear off the inner cortex along the inner side of the leaf sheath, place the torn endothelial layer on a glass slide, stain with 0.8% lactic acid aniline blue solution, and then use electric heating After heating the disc or an alcohol flame for a few seconds, examine it under a light microscope.

[0025] Result: After 2000 mature plant leaf sheaths of Drunken Horseweed were treated by the present invention, the detection rate of endophytic fungus was 100%. However, using the existing technology, the sample is very difficult to handle, and the detection rat...

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Abstract

The present invention relates to a method for detecting fungi grown in the plant body. Said invention has two method, and its first method is applicable to seedling, and includes the following steps: soaking grass sample in NaOH aqueous solution whose concentration is 5-15% for 3-5 hrs, then making staining treatment and detection; and its second method is applicable to plant, and includes the following steps: firstly, boiling sample leaf sheath in boiling water for 4-8 min, strip-taking endodermis along inside of leaf sheath, then staining endodermis and making detection. Said invented method is applicable to detection of grass whose texture is harder, and its detection accuracy is high.

Description

technical field [0001] The invention relates to a detection method, specifically a method for detecting fungi growing in plants. The method of the present invention includes dyeing the grass sample with a dye, and then placing the dyed sample under a light microscope for detection, or heating the dyed sample under a light microscope for detection. Background technique [0002] Endophytes are a group of fungi that live inside plants without showing external symptoms. Infestation of grass grasses with endophytic fungi can cause poisoning and even death in consuming livestock. According to the statistics of the United States and New Zealand, the economic losses caused by the disease and death of cattle and sheep due to the endophytic fungus (Neotyphodium coenophialum) and ryegrass endophytic fungus (Lolium perenne) amount to as much as 600 million U.S. dollars every year. It has been found that endophytic fungi contained in Achnatherum inebrians growing in northern my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/29
Inventor 李春杰南志标
Owner LANZHOU UNIVERSITY
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