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'Heat shock protein special extraction kit' for biological therapy

A heat shock protein and complex technology, applied in the biological field, can solve the problem of difficult to stimulate CTLs to activate and initiate cellular immunity, and achieve the effect of good specificity, good generality and good safety.

Inactive Publication Date: 2005-07-20
BEIJING ZHONGTIANKANGTAI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although tumor cells can stimulate the body to produce antibody responses, it is difficult to stimulate CTLs to activate and initiate cellular immunity, which is one of the reasons why tumor cells can escape the body's immune surveillance

Method used

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  • 'Heat shock protein special extraction kit' for biological therapy

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] Sample preparation:

[0043] Cut the tissue block into small pieces, put it in a homogenizer and homogenize it in an ice bath, rinse the homogenizer with pre-cooled equilibrium solution and collect the slurry in the homogenizer. Centrifuge to remove large pieces of tissue and cell debris in the slurry, and take the supernatant and add it to the affinity chromatography column. Keep the flow rate at the lower end of the column less than 0.5ml / min. During this period, you can also take measures to interrupt the outflow several times for 5 minutes to ensure that the HSP in the supernatant reacts fully with the resin-coupled antibody. When all the tissue cell slurry above flows into the resin, then wash the affinity column with the balance solution until the non-specific protein is completely washed from the column, and the UV absorption value is OD 280 <0.02.

[0044] The eluent can dissociate the HSP protein that reacts with the resin-coupled HSP antibody in the affinity...

Embodiment 1

[0054] Purpose:

[0055] Use the "Special Heat Shock Protein Extraction Kit" to quickly, efficiently and specifically extract HSPPC from surgically resected tumor tissues of breast cancer patients. The heat shock protein extracted from the tumor tissue of a breast cancer patient is combined with the tumor-specific antigen produced by the mutation of tumor cells. After purification, the patient's tumor antigen is injected back into the patient's body to mobilize the patient's immune system to respond The immune monitoring of the patient's own tumor stimulates the patient's own humoral immunity and cellular immunity to specifically identify and attack the tumor cells that the patient suffers from, removes tumor cells that have spread and diffused in various parts of the body, and achieves biological treatment of tumors the goal of. The "Heat Shock Protein Special Extraction Box" can facilitate the extraction of HSPPC by the hospital's biotherapy department to provide personaliz...

Embodiment 2

[0085] Purpose:

[0086] Use the "Heat Shock Protein Special Extraction Kit" to quickly, efficiently and specifically extract HSPPC from pathological organs and tissues that died due to infection with porcine enzootic pneumonia to prevent other pigs from being infected with porcine enzootic pneumonia and to treat infected pigs pigs for treatment.

[0087] Porcine enzootic pneumonia is an epidemic disease caused by mycoplasma, and the mortality rate of pigs after infection is very high. The disease is a common disease in intensive farms, and every time it occurs, it will bring significant economic losses to the farm.

[0088] Prepare the affinity resin in the "Special Extraction Kit for Heat Shock Proteins":

[0089] Purchase activated sepharose resin CNBr-Sepharose 4B.

[0090] Pre-coupling treatment:

[0091] Weigh 100g CNBr-Sepharose 4B and fully soak the swelling gel resin with 1mMo / L hydrochloric acid and then wash it with coupling buffer (0.1Mol / L NaHCO3, 0.1Mol / L Na2...

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Abstract

The present invention relates to one kind of specific heat shock protein extracting kit, and the extracting kit consists of heat shock protein affinity resin or heat shock protein affinity chromatographic column and may have matched mother liquid for affinity chromatography. The present invention aims at extracting heat shock protein or heat shock protein-polypeptide complex conveniently, fast, effectively and specifically from tissue or cell. The heat shock protein-polypeptide complex antigen extracted with the heat shock protein extracting kit is used in preventing and treating diseases caused by self cell mutation, foreign pathogen infection, autoimmune deficiency, etc.

Description

technical field [0001] The invention belongs to the field of biotechnology. technical background [0002] The reason why higher organisms can survive in a complex environment is because the body has the ability to resist foreign pathogens and eliminate endogenous mutant cells. Once this ability is reduced or disappears, the organism will become sick or cause death. [0003] The immune system of higher organisms such as mammals and humans has two mechanisms of humoral immunity and cellular immunity. One is humoral immunity mediated by B lymphocytes. B lymphocytes develop and mature in the spleen and bone marrow. Antibodies produced by B lymphocytes can specifically recognize and bind exogenous biomacromolecules, blocking exogenous body damage to the body. The complex formed by the antigen-antibody can stimulate the body's complement response and cellular response, and phagocytize and degrade the pathogen carrying the foreign biological macromolec...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P35/00C07K1/14
Inventor 韩苏许佐良
Owner BEIJING ZHONGTIANKANGTAI BIOTECH
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