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Pearl oyster monomer polysaccharide ZBP-I and extraction method and uses in preparation of drugs for tumor

A technology of ZBP-I and polysaccharides, which is applied in the field of monomer polysaccharides and its application in pharmaceuticals, and can solve the problems that the research of polysaccharides has not been reported yet.

Inactive Publication Date: 2004-11-10
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from the prior art that the mussel meat extract is only a mixture containing multiple components, and the crude polysaccharide extracted is only a class of polysaccharides, but further research on polysaccharides has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The extraction and separation method of embodiment 1. Pearl mussel monomer polysaccharide ZBP-I, its extraction step is:

[0022] (1) Dissolve 0.5 g of pearl mussel crude polysaccharide powder in distilled water, add it to the top of the DEAE-cellulose column, and slowly lower it until the polysaccharide solution completely enters the fiber column, then use H 2 O, 0.05mol of borax, followed by 0.001, 0.005, 0.01, 0.015mol of Na 2 CO 3 Solution elution, collect each eluate;

[0023] (2) The eluate is detected by UV, and the peaks of each eluate are collected and merged, and ultrafiltered with a molecular weight ultrafiltration membrane of 100,000, and the fraction with a molecular weight above 100,000 is cut off, and filtered with a 0.2um membrane;

[0024] (3) Vacuum-dry each ultrafiltration cut-off part at 40° C. to obtain polysaccharides I, II and III respectively, and polysaccharide I is the most obtained part;

[0025] (4) Take polysaccharide I 100mg and dissolve...

Embodiment 2

[0027] Embodiment 2, the antitumor effect of pearl mussel monomer polysaccharide ZBP-I

[0028] (1) Inhibitory effect of monopolysaccharide ZBP-I of pearl mussel on tumor growth in tumor-bearing mice

[0029] 1. Experimental materials

[0030] Animals: ICR mice, Wistar rats, provided by the Experimental Animal Center of Nanjing University of Traditional Chinese Medicine,

[0031] Reagents: RPMI-1640, calf serum, penicillin, streptomycin sulfate, MTT, DMSO (Sigma), sodium pentobarbital, pBR322 DNA / Hae III marlers, Proteinase K, RNaseA (Huamei Bioengineering Company), Agrose ( Spanish, 20010210), Tris (Amresco aliquots), bromophenol blue, ethidium bromide, MCDB131 medium, EACA, fibrinogen, thrombin.

[0032] Pearl clam monomer polysaccharide ZBP-I, Yunzhi polysaccharide capsule, cyclophosphamide.

[0033] Cell line: S 180 Sarcoma (provided by Shanghai Institute of Cell Biology, Chinese Academy of Sciences),

[0034] Equipment: 96, 24-well plate, plate (NUNC), syringe dispos...

Embodiment 3

[0063] The experimental results in Table 2 show that the formation of vascular collaterals in rat vascular rings was significantly inhibited under the action of polysaccharides, that is, the polysaccharide ZBP-I can inhibit the continued growth of tumors by affecting the formation of blood vessels during tumor growth. Embodiment 3 Pearl mussel monomer polysaccharide ZBP-I is to the influence of immune system

[0064] (1) Effects of monopolysaccharide ZBP-I of pearl clam on leukocyte level in immunocompromised mice

[0065] 1. Experimental materials

[0066] Animals: ICR and Balb / c strain mice, half male and half male, weighing 20±2 g, provided by the Experimental Animal Center of Nanjing Medical University and the Department of Animals of the Military General Hospital.

[0067] Reagents: 2,4-dinitrochlorobenzene (A.R), acetone (A.R), Na 2 S, cyclophosphamide, hydrocortisone injection, trypan blue, absolute ethanol (A.R), EDTA-2Na salt.

[0068] RPMI-1640, calf serum, Trypsi...

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Abstract

The invention discloses a process for separating and purifying pearl oyster monomer polysaccharide ZBP-I from the crude polysaccharides extracted from pearl oyster, which comprises, dissolving the crude polysaccharide powder into water, charging into cellulose columns, eluting with dissolvent, subjecting the eluent to hyperfiltration by ultrafilter membrane and vacuum drying or lyophilization to prepare pearl oyster polysaccharide I powder, dissolving the polysaccharide I in water, separating through Sephadex G-200 column, the pearl oyster monomer polysaccharide ZBP-I is finally obtained from the eluent through alcohol deposition of polysaccharide, filtering, vacuum drying or freeze drying. The monomer polysaccharide ZBP-I exhibites appreciable enhancing action for the anti-tumor activity of the natural killer NK cells.

Description

1. Technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a monomeric polysaccharide extracted from pearl clam mollusk and its application in pharmacy. 2. Background technology [0002] Mussels for cultivating freshwater pearls generally use shellfish Hyriopsis cumingii (Lea) , Cristaria plicata (Lea) , and Anodontawoodiana (Lea) ]. After being harvested, the mother body (soft body) of the cultured pearls usually dies, and is discarded as waste or a very small part of it is processed into feed. It has been reported that the polysaccharide extracted from the soft body of the triangular clam has an antitumor effect (Chinese Journal of Modern Applied Pharmacy, Volume 20, Issue 1, February 2003), the effect of mussel extract on the immune function of mice (Journal of Chengde Medical College, V01.16, NO.4, 1999), He Mussel meat extract can increase the phagocytic index of mouse macrophages, increase the level of serum hemolysin i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00C08B37/02
Inventor 吴皓陈文星张莉池玉梅狄留庆陆茵蔡皓
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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