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Multifunctional fluorescent, magnetic Nano material and preparation method

A nanomaterial and fluorescent nanotechnology, applied in the field of fluorescent magnetic multifunctional nanomaterials and their preparation, can solve the problems of being easy to be photobleached, restricting the application of fluorescence methods, and narrow excitation spectrum

Active Publication Date: 2004-10-27
WUHAN UNIV
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Problems solved by technology

The use of fluorescent reagents for biomarking and cell development and imaging has always been an active research field, but the current general-purpose organic dye-based fluorescent reagents have obvious disadvantages such as low fluorescence intensity, narrow excitation spectrum, and easy to be photobleached.
These shortcomings seriously restrict the application of fluorescence methods in medical experiments and clinical detection.
Magnetic separation technology for biological analysis is not yet popular

Method used

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  • Multifunctional fluorescent, magnetic Nano material and preparation method
  • Multifunctional fluorescent, magnetic Nano material and preparation method
  • Multifunctional fluorescent, magnetic Nano material and preparation method

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Embodiment Construction

[0034] specific implementation

[0035] The following examples illustrate the specific implementation of the present invention, but the content of the present invention is not limited to the examples given.

[0036] An example of how to make it is as follows:

[0037] 1. Use the method of soap-free emulsion polymerization to prepare styrene / acrylamide copolymerized microspheres with uniform size and excellent appearance. The particle size is generally 50nm-2um, and the particle size can be controlled from 100nm-200nm by experimental conditions.

[0038] 2. Use the method described in our invention patent application document No. 02139152.1 to synthesize high-quality fluorescent nanometer quantum dots (e.g, CdSe / ZnS,) with a controlled particle size of 4nm-8nm. The synthesis process is safe, non-toxic and low in cost.

[0039] 3. Synthesize magnetic nanoparticles (e.g, γ-Fe 2 o 3 , Fe 3 o 4 ), with an average particle size of 8 nm.

[0040] 4. Prepare a chloroform / n-buta...

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Abstract

A multifunctional nano-class magnetic fluorescent material is prepared through wrapping nano fluorescent material (CdSe / ZnS, 4-8 nm) and nano magentic material (gamma-Fe2O3, 4-20nm) by high-molecular microbead in water or oil phase. Its advantage is high intensity and long life of fluorescence.

Description

technical field [0001] This paper discloses a fluorescent magnetic multifunctional material and a preparation method thereof, and belongs to the technical fields of analytical chemistry, nanomaterials, synthetic chemistry, and bioanalytical chemistry. Background technique [0002] Multifunctional materials have always been a hot field of materials research, and nanomaterials have attracted more and more attention from scientists because of the peculiar properties endowed by nanometer size effects and quantum surface effects. The use of fluorescent reagents for biomarking and cell development and imaging has always been an active research field, but the current general-purpose organic dye-based fluorescent reagents have obvious shortcomings such as low fluorescence intensity, narrow excitation spectrum, and easy to be photobleached. These shortcomings seriously restrict the application of fluorescence methods in medical experiments and clinical detection. The use of magnetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08K3/22C08L25/04C09K11/02
Inventor 庞代文谢海燕刘毅左超张志凌
Owner WUHAN UNIV
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