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Plane substrate packed polymer composition and its application

A polymer and coating technology, used in biochemical equipment and methods, microbial assay/inspection, instruments, etc., can solve problems such as high cost, high non-specific binding activity, and complexity

Inactive Publication Date: 2004-07-21
CHENGDU KUACHANG SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-activation polymers have high ligand binding activity, this high activity is often manifested as high non-specific binding activity
In addition, the polyamino acid-coated substrate also has the problem of coating stability
In order to solve the problem of excessive activation of the coating polymer, some people adopt the method of copolymerization to introduce inactive polymer chains in the polymer with high activation degree, but this solution seems to be too complicated and the cost is too high (refer to the application published by the US Patent Office No. 20020128234)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Optimizing the preparation of substituted polymers

[0058] (1). Preparation of DEAE-dextran / DEAE-agarose:

[0059] Dextran was purchased from Shanghai Chemical Reagent Company, agarose was purchased from Sigma Company, and DEAE was purchased from Baoding Jiahe Fine Chemical Co., Ltd.

[0060] For the preparation method, refer to an article written by one of the inventors of the present invention (COATED SILICA SUPPORTS FOR HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY OF PROTEIN, Journal of Chromatography, 476, (1989) 195-203,).

[0061] DEAE substitution rate determined by acid-base titration: 7-14%.

[0062] (2). Preparation of hydrazide-polyacrylamide:

[0063] Hydrazide-polyacrylamide was prepared according to the method of JK. Inman and HM Dintzis (Reference: The derivatization of cross-linked polyacrylamidebeads, Am. J. Epidemiol. 143: 263-268, 1969). The nonionic polyacrylamide used in this example is produced by Beijing Xitao Technology Development Co....

Embodiment 2

[0067] Embodiment 2: Preparation of polymer-coated film base

[0068] The coating polymers used in this example are shown in Table 1, and the substrates used are respectively microscopic slides (U.S. TeleChem International, Inc) used to prepare chip bases and 96-well microwells used to prepare enzyme-labeled plate bases Board (Shenzhen Jincanhua Industrial Co., Ltd.).

[0069] 1). Coating of polymer on the substrate

[0070] Dissolve the coating polymers in Table 1 (including polyvinylpyrrolidone, polysaccharide derivatives and polyacrylamide derivatives) into PBS buffer respectively to obtain a coating polymer aqueous solution with a concentration of 1% (W / V) . Then they were diluted 40-100 times respectively. The microscope slides were placed in the obtained dilution of the coated polymer, coated overnight at room temperature, washed and dried, and used after passing the quality inspection. Add 100-200 ul of the obtained coating polymer dilution to each well of a 96-well...

Embodiment 3

[0079] Embodiment 3: the preparation of chip

[0080] The coating polymer used in this example is selected from Table 1, and the sheet base used is selected from Table 2. Microscope slides as substrates and amino slides as reference photobases were purchased from TeleChem International, Inc., USA. The ligands used in this embodiment include: HCV antigen (Beijing People's Hospital Institute of Liver Diseases), HIV 1+2 Antigen (Beijing People's Hospital Institute of Liver Diseases) and EBV antigen. EBV antigen is a peptide antigen synthesized according to the method of D.Trabchand-Bunel (Detection of human antibodies using "convergent" combinatorial peptide libraries or "mixotopes" designed from anonvariable antigen: application to the EBVviral capsid antigen p18, D.Trabchand-Bunel et al., J. Peptide Res, 52.1998, pp495-508).

[0081] Before the ligand or ligand / polymer is coated, a highly hydrophobic silicone coating (Chengdu Chenguang Chemical Design Institute) is coated on...

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PUM

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Abstract

The present invention relates to an encapsulation composition for encapsulating plane substrate, a polymer encapsulating chip base, a reactor containing polymer encapsulation and a device including the reactor especially chip device. The encapsulation polymer provided by the present invention consists of surface activated agent of high polymer and optimization substituted polymer which have enough high activity of combination genin and minimizing activity of non-specific combination.

Description

technical field [0001] The present invention relates to a coating composition for coating a planar substrate, a polymer-coated sheet base, a polymer-coated reactor and a device for a specific reactor, especially a quantitative or / and qualitative detection device, especially a biochip. Background technique [0002] An increasingly important class of devices containing specific reactors, especially quantitative or / and qualitative detection devices, are devices containing specific reactors based on planar substrates. At present, the main way to prepare this type of reactor is as follows: first, the surface of the planar substrate is modified to prepare a substrate with ligand-binding activity, and then the ligand is fixed on the substrate. Depending on the matrix used and the immobilization method of the ligand, there can be different types of reactors and devices, such as enzyme-labeled microplates formed by immobilizing the ligands in a polystyrene microwell plate in a random...

Claims

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Application Information

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IPC IPC(8): C08L39/06C12Q1/24G01N33/545
Inventor 邹方霖陈春生陈宁王建霞
Owner CHENGDU KUACHANG SCI & TECH CO LTD
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