Method for building rat model of lung scaly cancer by filling carcinogenic iodized oil to bronchus of left lung

Abstract

Carcinogenic iodized oil solution is prepared by dissolving methyl cholanthrene and diethylnitrosamine in iodized oil. Filling the said iodized oil into bronchus of left lung of Wistar rat induces positioned lung scaly cancer. At 3 days after the said iodized oil being filled, hyperplasia is occurred on epithelium mucosae of bronchus. At 5, 6 days, squamous metaplasia is occurred. At 7-9 days, atypical hyperplasia is occurred. At 13-16 days, early invasive carcinoma is occurred. After 22 days, wet ability scaly cancer is formed. The invention can be utilized in researching pathogenesis, new drugs and new therapeutic methods. Advantages of the invention are: inducing lung scaly cancer at specific position, high rate of inducing cancer and high repeatability. The method is easy of operation, economy as a good model for researching lung cancer.

Description

Technical field: [0001] The invention relates to the establishment of an animal model of the disease, which can be used for research on the etiology, pathogenesis, transfer rule, pharmacological action, curative effect and side effects of new drugs and the like of squamous cell carcinoma of the lung. Background technique: [0002] Lung cancer is one of the common malignant tumors that seriously endanger human life. In recent years, its morbidity and mortality have been on the rise. Although the research on lung cancer has made great progress in the past few decades, the means of early diagnosis and treatment are still Very limited, one of the main reasons is the lack of suitable animal models as research objects, making it difficult to conduct a series of in-depth studies. Therefore, in order to further study the etiology, pathogenesis, metastasis and treatment methods of lung cancer, it is necessary to establish a suitable animal lung cancer model. [0003] Existing method...

AI Technical Summary

Problems solved by technology

[0003] Existing methods for establishing animal lung cancer models include: (1) isolating full-length ovine lung adenomatosis retroviral provirus from the genomic DNA library of naturally occurring ovine lung adenomatosis, transfecting neonatal cells in vivo through intratracheal inoculation. In sheep, it can induce ovine lung adenomatosis similar to human bronchioloalveolar carcinoma. The disadvantage of this method is that it is technically difficult and complicated to operate.

(2) A new gene targeting method was used to establish a mouse strain carrying the K-ras allele oncogene, which can randomly generate an activated K-ras mutant gene through a natural recombination process and have a high sensitivity to many tumors Susceptibility, especially early lung cancer, the disadvantage of this method is high technical requirements, difficult and complicated operation

(3) Fresh canine tumor cells were inoculated into the lungs of dogs intraarterially or percutaneously, and immunosuppressed with cyclosporine. After 6-10 weeks, there were scattered tumor nodules visible to the naked eye in the lungs. The downside of the method is that it happens so differently from humans

(4) Coal tar pitch (CPT) can also be used to induce lung cancer through bronchial perfusion. The disadvantage of this method is that no iodine agent is used, which is not conducive to the observation and positioning of the induction process, and multiple perfusions are required. month