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A light scattering detector and capillary tube electrophoresis device for the detector

A technology of capillary electrophoresis and detectors, which is applied in the direction of scattering characteristic measurement, instruments, and measuring devices, and can solve problems such as complex detector equipment

Inactive Publication Date: 2003-10-15
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire detector equipment is complex, not suitable for use with flow injection analyzers and capillary electrophoresis instruments

Method used

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  • A light scattering detector and capillary tube electrophoresis device for the detector
  • A light scattering detector and capillary tube electrophoresis device for the detector
  • A light scattering detector and capillary tube electrophoresis device for the detector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Quantitative detection of hexavalent chromium ion Cr(VI) in the solution in conjunction with a flow injection analyzer.

[0031] The complete set such as Figure 5 shown. exist figure 1 On the basis of the common flow injection analyzer (11), the waste liquid pool (12) is connected. The light source (1) used in this device is a He-Ne laser with a wavelength of 632.8 nanometers and a power of 50 milliwatts. The specification of the optical fiber (7) used is 600 microns in outer diameter.

[0032] Rhodamine B (Rhodamine B, RhB) has very weak laser Rayleigh scattering signal in acidic solution. But in the presence of excess iodide I - After addition, a simple ionic association [RhB][I] was formed and the signal intensity began to increase. At this time, if a trace amount of Cr(VI) is added, the laser Rayleigh scattering signal is significantly enhanced, which is due to the oxidation of Cr(VI) - produce I 2 , with I - combined to form I 3 - , react ...

Embodiment 2

[0034] Embodiment 2: Combined with capillary electrophoresis to detect hexavalent chromium ion Cr(VI) in the solution.

[0035] exist Figure 5 On the basis of , change the flow injection analyzer (11) into a self-assembled capillary electrophoresis instrument, and the others are the same. The detailed structure of the capillary electrophoresis instrument is as follows:

[0036] Please combine Figure 6, (14) is a cuboid plexiglass base, the stainless steel needle of suitable thickness is burned, and the straight main channel is scalded on the plexiglass block top, and the left end (15) of this main channel is arranged with a section of internal diameter 530 microns Capillary, one end of the capillary is placed in the channel (15), and the other end is directly connected with the flow cell (6) in the light scattering detector of the present invention. A section of capillary with an internal diameter of 360 microns is installed in the right channel (16) to transmit the curre...

Embodiment 3

[0045] Embodiment 3: Combined use with micro-liquid chromatography system to detect hexavalent chromium ion Cr(VI) in solution.

[0046] exist Figure 5 On the basis of the above, the flow injection analyzer (11) is changed into a micro-liquid chromatography system, and the others are the same. About the micro liquid chromatography system structure such as Figure 8 Shown: (26) is a miniature liquid chromatography system, (27) is a three-way pool, and (19) is a low-pressure air pump. The principle of the device for detecting Cr(VI) is the same as that in the second embodiment. See Example 2 for the preparation of the current-carrying reagent, and push it into the channel with an air pump. With double redistilled water as the mobile phase, 10 microliters of 0.353 microgram / ml Cr(VI) solution was injected. The results show that it can be used in the detection of micro liquid chromatography system.

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Abstract

The light scattering detector for in-situ microanalysis collects the Rayleigh scattering signal, which is mach greater than Raman scattering signal, for detection. In the design of optical path, theinterference caused by Raman scattering signal is non-significant. The liquid specimen to be detected needs no atomizing or evaporation in advance. The light scattering detector of the present invention may be used alone for microanalysis, together with capillary electrophoresis apparatus, or together with flow injection analyzer and liquid phase chromatograph for in-situ post-column detection and analysis.

Description

technical field [0001] The invention relates to a light scattering detector system, in particular to an on-line micro-analysis light scattering detector and a capillary electrophoresis instrument used in conjunction with the detector. Background technique [0002] When the incident light is irradiated on a particle whose size is much smaller than the incident wavelength, the photons collide with the particle and scatter, the Rayleigh scattering signal is generated based on the elastic collision, and the Raman scattering signal is generated based on the inelastic collision. The Raman scattering signal is very weak, only a few hundredth to one ten-thousandth of the Rayleigh scattering signal. However, in practical applications, the Raman scattering signal is usually collected to obtain a Raman spectrum, and the Rayleigh scattering signal is used as an interference factor to try to eliminate it. [0003] Under the condition of other analysis conditions such as incident wavelen...

Claims

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Application Information

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IPC IPC(8): G01N21/47G01N27/447
Inventor 陈义王强
Owner INST OF CHEM CHINESE ACAD OF SCI
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