Rhodococcus erythropolis and application in removing sulfur element in surfide thereof

A technology of Rhodococcus erythropolis and compound, applied in application, bacteria, plant genetic improvement and other directions, can solve the problems of no ownership of fungi and impossible industrial production.

Inactive Publication Date: 2003-05-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many researches on this type of fungus are carried out by borrowing Rhodococcus from abroad. Without the ownership of this type of fungus, it is impossible to directly apply it to industrial production.

Method used

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  • Rhodococcus erythropolis and application in removing sulfur element in surfide thereof
  • Rhodococcus erythropolis and application in removing sulfur element in surfide thereof
  • Rhodococcus erythropolis and application in removing sulfur element in surfide thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0042] Pick the LSSE8-1 strain cultured on the nutrient slant and add it to 25 ml of basal medium. The composition of the medium: deionized water 1000 ml, KH 2 PO 4 2.44 g; Na 2 HPO 4 12H 2 O 14.03 g; NH 4 Cl2.00g; MgCl 2 ·6H 2 O 0.36 g; CaCl 2 2H 2 O 0.001 g FeCl 3 ·6H 2 O 0.001 g MnCl 2 4H 2 O 0.004 g; Glucose 10 g, Na 2 SO 4 1mmol / L. After culturing at 30°C and 150 rpm for 24-48 hours, it was added to 500 ml of basal medium, and after 48-72 hours, it was centrifuged to obtain bacterial cells. Place the bacterial cells in 50 ml of physiological saline containing 1 mmol / L DBT, mix on a shaker for 2-4 hours, and centrifuge to obtain the bacterial cells again. Example 3: Obtaining LSSE8-1 Active Stem Cells

Embodiment 3

[0043] Pick the LSSE8-1 strain cultured on the nutrient slant and add it to 25 ml of basal medium. The composition of the medium: deionized water 1000 ml, KH 2 PO 4 2.44 g; Na 2 HPO 4 12H 2 O 14.03 g; NH 4 Cl2.00g; MgCl 2 ·6H 2 O 0.36 g; CaCl 2 2H 2 O 0.001 g FeCl 3 ·6H 2 O 0.001 g MnCl 24H 2 O 0.004 g; Glucose 10 g, Na 2 SO 4 1mmol / L. After culturing at 30°C and 150 rpm for 24-48 hours, it was added to 500 ml of basal medium, and after 48-72 hours, it was centrifuged to obtain bacterial cells. Place the bacteria in 50 ml of physiological saline containing 0.1 mmol / L DBT, mix on a shaker for 2-4 hours, and centrifuge to obtain the bacteria again. Freeze and vacuum dry to obtain active stem cells. Example 4: Strain LSSE8-1 removes sulfur in the simulated system DBT.

Embodiment 4

[0044] Add DBT to dodecane to make the concentration reach 0.2mmol / L. Pick the LSSE8-1 strain cultured on the nutrient slant and add it to 25 ml of basal medium. The composition of the medium: deionized water 1000 ml, KH 2 PO 4 2.44 g; Na 2 HPO 4 12H 2 O 14.03 g; NH 4 Cl 2.00 g; MgCl 2 ·6H 2 O 0.36 g; CaCl 2 2H 2 O 0.001 g FeCl 3 ·6H 2 O 0.001 g MnCl 2 4H 2 O 0.004 g; glucose 10 g, DBT concentration 1 mmol / L. After culturing at 30° C. and 150 rpm for 24-48 hours, the simulated oil phase and bacterial culture were mixed at an oil-to-water ratio of 1:3. After culturing at 30° C. and 150 rpm for 24-48 hours, the sulfur is completely removed, and the product is HBP (as shown in FIG. 5 ). Example 5: Bacterial strain LSSE8-1 is used for deep desulfurization of diesel oil after hydrodesulfurization

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Abstract

The invented Rhodococcus erythropolis LSSE8-1 is preserved in "general Microbe Centre, Chinese Microbe Culture Preservation & Management Committee" in 9,10,2001, its preservation number is CGMCCNO.0643; its Gram reaction is positive, partial acidoresistant and aerobic; after 14 hr. of sheet-inserting culture at 30 deg.C on the glucose asparagine agar and nutrient agar, its hypha is started breaking from centre of colony, after 24 hr. the globular or rhabdoid corpuscle can be produced, after 72 hr. the circumference of colony can be continuously grown, there is not aerial mycelium, occasionally it has ramification, it has no requirement for vitamin B1, it contains meso diaminopimelic acid, and has characteristic arabinose and galactose, its cell wall belongs to IV type.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and in particular relates to a rhodococcus erythropolis and its application in removing sulfur from sulfur-containing compounds. Background technique [0002] The main elements of petroleum are carbon and hydrogen, which together reach 96-99%, and the remaining 1-4% are sulfur, nitrogen, oxygen and trace metal elements. Sulfur exists in the form of inorganic sulfur and organic sulfur in petroleum. Inorganic sulfur includes dissolved and suspended monomeric sulfur, hydrogen sulfide and pyrite, etc., while organic sulfur includes various mercaptans, thioethers, disulfides and thiophenes and their derivatives. Generally speaking, among organic sulfur-containing compounds, thiophenes are the most abundant. Many small molecular sulfur-containing organics with low boiling points are relatively easy to remove by simple chemical methods. Therefore, many deep desulfurization r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/31C12S1/02C12S99/00
Inventor 缑仲轩刘会洲邢建民罗明芳李珊陈家镛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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