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Extraction separation method of algae polysaccharide

A separation method and technology for algal polysaccharide, applied in the field of extraction and separation of algal polysaccharide, can solve problems such as loss of activity of polysaccharide protein and destruction of polysaccharide components, and achieve the effects of maintaining biological activity, reducing production cost and increasing utilization rate

Inactive Publication Date: 2003-04-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The previous algal polysaccharide extraction and purification technology mainly focused on the extraction of exopolysaccharides, and the hot water soaking method was commonly used, for example, the polysaccharide extraction technology described in Chinese Patent Publication No. 1220999, which often resulted in the loss of activity of polysaccharide proteins
Although the technology used in Chinese Patent Publication No. 1280855 mentions the problem of wall breaking, this method inevitably leads to the denaturation of glycoproteins and the hydrolysis of some polysaccharides
Another example is the method described in Chinese Patent Publication No. 1112128, although avoiding the damage of glycoprotein caused by excessive temperature, it is possible to destroy some polysaccharide components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 500g of fresh spirulina algae mud, add 1000ml of water, and crush at room temperature for 15 minutes. Centrifuge at 7500rmp. for 15 minutes, and discard the precipitate. Add 0.5 g of papain, and incubate at 45°C for 5 hours. Add an equal volume of chloroform-isoamyl alcohol to the sample (the volume ratio of chloroform to isoamyl alcohol is 5:1), shake vigorously, let stand for 2 hours, centrifuge at 7500rmp for 10 minutes, and repeat the above steps until no precipitation occurs. The supernatant was precipitated with 2-3 times the volume of ethanol (take the supernatant and add ethanol until no precipitation). Finally, the precipitate was washed several times with acetone and dried to obtain 8 g of crude polysaccharide with a polysaccharide yield of 1.6%.

[0024] The above crude polysaccharide was dissolved in 20 ml of Tris-HCl with a pH value of 7.6 and a concentration of 10 mmol / L, and centrifuged at 7500 rpm for 10 minutes to discard the precipitate. The su...

Embodiment 2

[0026] Take 50 grams of spirulina algae powder, add 1000ml of phosphate buffer, and crush at room temperature for 20 minutes. Centrifuge at 7500rmp for 15 minutes and discard the precipitate. Add an equal volume of chloroform-isoamyl alcohol (the volume ratio of chloroform to isoamyl alcohol is 5:1) to the sample, shake vigorously, let stand for 2 hours, centrifuge at 7500rmp for 10 minutes, repeat the above steps 4 times until there is no precipitation. The supernatant was precipitated with 2-3 times the volume of ethanol (take the supernatant and add ethanol until no precipitation), washed the precipitate with acetone, and dried to obtain 4 g of crude polysaccharide with a yield of 8%.

[0027] The above crude polysaccharide was dissolved in 20 ml of Tris-HCl with a pH value of 7.6 and a concentration of 10 mmol / L, and centrifuged at 7500 rpm for 10 minutes to discard the precipitate. The supernatant was loaded on a DEAE-Sepharose Fast Flow column, using Tris-HCl with a pH ...

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Abstract

The extraction and separation method of algae polysaccharide is characterized by that the present invention utilzies the characteristics of polysaccharide and glucoprotein which are dissolved in water, adopts water extraction method to make extraction, uses ethyl alcohol to make fractional precipitation to make separation, finally adopts the anion-exchange column and gel exclusion chromatography to purity polysaccharide, in which the biological activity of protein and protein polysaccharide can be retained.

Description

technical field [0001] The invention relates to a method for extracting and separating algal polysaccharides. Background technique [0002] Seaweed is the most widely distributed photosynthetic prokaryote in nature and has high nutritional value. Seaweed protein contains various essential amino acids, vitamins, various trace elements, γ-linolenic acid and some natural pigments. Algae polysaccharides have high medicinal value, and have significant effects in improving the non-specific immune function of the human body, anti-hypoxia, anti-tumor, anti-radiation, anti-aging, lowering blood sugar, and lowering blood fat. The active substances in seaweed polysaccharide (SP) are mostly water-soluble acidic and neutral polysaccharides composed of D-glucose, D-mannose, D-galactose, D-glucuronic acid and other monosaccharides. The previous algal polysaccharide extraction and purification technology mainly focused on the extraction of exopolysaccharides, and t...

Claims

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Application Information

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IPC IPC(8): C08B37/04
Inventor 龚兴国曾冬云郭建军
Owner ZHEJIANG UNIV
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