Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use

A compound and hypoglycemic technology, applied in the directions of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as unreported physical and chemical constants and structures, and achieve simple extraction and separation methods, high yields, blood sugar lowering effect

Inactive Publication Date: 2003-02-26
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI +1
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mao Junhao et al. (Journal of Immunology, 1996, 4: 233) reported that the polysaccharide component PAM of Atractylodes macrocephala regulates the immune function of mouse spleen lymphocytes, and the physical and chemical constants and structures of this component have not been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use
  • Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] After crushing the rhizomes of Atractylodes macrocephala, weigh 200g, add 2000ml deionized water, and soak for 48 hours at room temperature, during which it is stirred for several times to facilitate the leaching of active ingredients. The soaking liquid is first filtered with four layers of gauze, and then the filtrate is centrifuged at a speed of 4500r / min, and the centrifugal time is 8min. The supernatant was concentrated under reduced pressure, with a temperature control range of 50°C-55°C, and a concentrated volume to 600ml. In 600ml concentrated solution, add 3600ml of 95% ethanol for alcohol precipitation, the alcohol precipitation time is 24 hours. After the alcohol precipitation is over, the supernatant is gently decanted, and the sedimented part is centrifuged at a speed of 4500r / min and a centrifugal time of 8min. Take out the precipitate and add 500ml of deionized water to dissolve, after dissolving, centrifuge to remove the insoluble matter. The supernatant is ...

Embodiment 2

[0023] Wash 1 kg of crude drug Baizhu, dry, crush, soak in 10 liters of deionized water at room temperature for 24 hours, and filter. The residue was soaked in 5 liters of deionized water for 24 hours, filtered, and the filtrate was combined, and concentrated under reduced pressure at 60°C to 2-4 liters. Then it is precipitated with 95% ethanol, the final concentration of ethanol is 80%, and the alcohol is precipitated for 24 hours. Centrifuge, dissolve the precipitate with 2.5 liters of water, centrifuge, and dialyze in ion-free water for 48-72 hours. After concentration under reduced pressure at 60°C and freeze-drying, 12 grams of Baizhu sugar complex was obtained. Elemental analysis: C=37.99%, H=6.20%, N=2.19%; sugar content: 52.7%; uronic acid content: 15.9%; protein content: 16.7%.

Embodiment 3

[0025] Wash 500g of the crude drug Baizhu, dry, crush, soak in 5 liters of deionized water at room temperature for 24 hours, and filter. The residue was soaked in 3 liters of deionized water for 24 hours, filtered, and the filtrate was combined and concentrated under reduced pressure at 60°C to 2 liters. Then it is precipitated with 95% ethanol, the final concentration of ethanol is 85%, and the ethanol is precipitated for 24 hours. Centrifuge, dissolve the precipitate with 1 liter of water, centrifuge, and dialyzed against ion-free water for 48-72 hours. After concentration under reduced pressure at 60°C and freeze-drying, 6.2 grams of Baizhu sugar complex was obtained. Elemental analysis: C=45.02%, H=6.01%, N=1.05%; sugar content: 58.0%; uronic acid content: 28.1%; protein content: 11.0%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An active largehead atractylodes saccharide complex is produced through water soaking largehead atractylodes rhizome, organic solvent precipitating, water dissolving the precipitate, dialyzing or membrane separating the supernatant liquid, concentration and drying to obtain coarse product, column chromatographic separation of the coarse product to obtain fine largehead atractylodes saccharide compound. The process is simple and high in yield and is suitable for industrial production. The largehead atractylodes saccharide complex contains polysaccharide in 40-60 wt%, alduronic acid in 15-45 wt% and protein in 10-25 wt% and the polysaccharide includes glucose, galactose, mannose, arabinose and rhamnose. The largehead atractylodes saccharide complex has obvious blood sugar reducing effect and no toxic side effect.

Description

Technical field [0001] The invention relates to a sugar compound extracted from Chinese medicinal materials, a production method and application, and in particular to a Baizhu sugar compound, a production method and application. technical background [0002] Atractylodes macrocephala is a traditional Chinese medicinal material, which has the effects of "tonifying qi and invigorating the spleen, eliminating dampness and diuresis, antiperspirant, and anti-fetus. Atractylodes macrocephala contains volatile oils such as atractylone, atractylodes lactone and trace elements. There are few reports on chemical and biological activities. Gu Yucheng et al. (Chinese Herbal Medicine, 1992, 10: 507) isolated two polysaccharides from Atractylodes macrocephala with a molecular weight of 3.1×10 4 Mannan and molecular weight 1.1×10 4 There is no report of biological activity of the fructan. Chi Yumei et al. (Chinese medicinal materials, 2001, 9: 647) obtained a molecular weight of 1.36×10 5 And m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7004A61P3/10
Inventor 田庚元单俊杰
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com