Multispectral imaging gene chip scanning instrument

Abstract

The gene chip scanister mainly is used for detecting fluorescently-labeled post-hybridization gene biological chip, including the following steps: a laser beam is saped by linear shaper and passed through a reflector with slit to form a linear laser beam on the tested chip; the optical signal fed back by tested chip is passed through the reflector with slit and reflected, then passed through a photographic optical system and image in the slit diaphragm position; when it is excited by using laser beam with multiple wavelength, said signal is further passed through first imaging lens, dispersing element and second imaging lens and reflected on the detector of electric charge coupler with face array or line array. As compared with existent technology, it not only can simplify scanning direction, save detection time, but also can raise the resolution and accuracy of the detection.

Description

Technical field: [0001] The invention relates to a multispectral imaging gene chip scanner, in particular to a gene chip scanner using laser push-broom multispectral imaging. It is mainly used for detection after hybridization of fluorescently labeled gene biochips. It is generally believed that through the scanning and detection of biochips, including the comparison and analysis of the obtained signals by using corresponding software, people can take advantage of the advantages of high-throughput, simplicity, miniaturization, multi-parameters, intensification, and parallelization of gene chips. It opens up broad application prospects in many fields such as life science, basic medical research, disease diagnosis, new drug development, agriculture, food and environmental protection. Background technique: [0002] For fluorescently labeled biological gene chips, special gene scanners are required for post-hybridization detection. At present, dedicated gene chip scanners are ...

AI Technical Summary

Problems solved by technology

Its size and power consumption are relatively large

[0006] In addition, the above-mentioned prior art [1] and [2] these two kinds of gene chip scanners with PMT and CCD as detection elements also have some common shortcomings: for example, the object to be detected or the excitation laser beam must have two directions of XY The detection task can only be completed by the translational movement of the chip scanner, so the chip scanner must have a multi-dimensional motion mechanism; the obtained images need to be spliced ​​after computer data processing, and the accuracy of the motion mechanism and the positioning accuracy of the motion mechanism are very high. The process also becomes more complicated; the scanning of the whole chip takes a long time, so the efficiency is low; the cost of the whole machine is relatively expensive, etc.

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