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Injection for treating cerebral thrombus and preparation thereof

A cerebral thrombosis and injection technology, applied in the field of thrombolytic injection for the treatment of cerebral thrombosis and its preparation, can solve the problems of poor treatment effect, high price, insignificant effect, etc., and achieve the purpose of promoting nerve cell recovery, dilating blood vessels, and resisting platelets. The effect of adhesion aggregation

Inactive Publication Date: 2002-01-30
郝文学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Danshen injection, whose main ingredient is Danshen, has an effective rate of 80% in treating hyperlipidemia, is cheap, but the effect is not significant
Donglingxobin treats cerebral thrombosis, the effective rate is 90%, but the price is 400 yuan / bottle, the effect is good, and it cannot be used for a long time
The main ingredients of Xiaoshuan Tongluo Tablets are Chuanxiong, Danshen, Astragalus, etc., which can promote blood circulation and remove blood stasis, warm meridian and collaterals, and the treatment effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The first step: G75 chromatography column chromatography, after treatment with 0.2M NaOH, pack the column, use 0.2M disodium hydrogen phosphate buffer to balance the pH6.8, take Agkistrodon venom and dissolve it in 10ml of 0.2M disodium hydrogen phosphate buffer, The sample was loaded by centrifugation, loaded on the column, eluted with 0.02M disodium hydrogen phosphate buffer, collected with a fractional collector, and the collected enzyme activity peak was concentrated and freeze-dried. Agkistrodon venom is freeze-dried powder of Jiangsu-Zhejiang Agkistrodon venom.

[0057] The second step: Sephadex-A25 chromatography column chromatography, with 0.01M Tris-HCl buffer solution, PH7.0 soaks gel, in Tris (trishydroxymethylaminomethane)-HCl buffer solution (trishydroxymethylaminomethane The concentration in the buffer solution is 0.01M-0.05M, the pH value is 7.5), Sephadex (cross-linked dextran). It was then loaded into a column and equilibrated with 0.01 M Tris-HCl buff...

Embodiment 2

[0061] The first step: G75 column chromatography, after treatment with 0.15M NaOH, install the column, use 0.15M sodium dihydrogen phosphate buffer to balance pH 6.8, take Agkistrodon venom and dissolve it in 10ml of the above buffer, centrifuge and load the sample, put on the column, and use The 0.01M sodium dihydrogen phosphate buffer was eluted, collected with a fractional collector, and the collected enzyme activity peaks were concentrated and freeze-dried.

[0062] The second step: Sephadex-A25 chromatography column chromatography, soak the gel with 0.01M Tris-HCl buffer solution, pH7.0, then load it into the column, and equilibrate it with 0.01M Tris-HCl buffer solution. Dissolve the sample from the first step of chromatography in 0.01M Tris-HCl buffer and put it on the column, equilibrate the buffer, wash away impurity proteins with 0.01M NaCl buffer, change to 0.45M NaCl buffer for elution, and collect enzyme activity peak, concentrated and lyophilized. Step 3: Same a...

Embodiment 3

[0065] The first step: G75 column chromatography, after treatment with 0.3M NaOH, install the column, use 0.3M disodium hydrogen phosphate buffer to balance the pH6.8, take Agkistrodon venom and dissolve it in 10ml of the above buffer, centrifuge and load the sample, and put it on the column. It was eluted with 0.03M disodium hydrogen phosphate buffer, collected with fractional collector, and the collected enzyme activity peak was concentrated and freeze-dried.

[0066] The second step: Sephadex-A25 chromatography column chromatography, soak the gel with 0.01M Tris-HCl buffer solution, pH7.0, then load it into the column, and equilibrate it with 0.01M Tris-HCl buffer solution. Dissolve the sample from the first step of chromatography in 0.01 M Tris-HCl buffer and put it on the column, equilibrate the buffer, wash with 0.01M NaCl buffer to remove impurities, change to 0.8M NaCl buffer for elution, and collect enzyme activity peak, concentrated and lyophilized. Step 3: Same as ...

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Abstract

The preparation method of injection preparation containing above 70% of arginine lipase active component with the functions of dissolving thrombosis, anticoagulant, resisting platelet agglutination and improving microcirculation for curing thrombosis, encephalatrophy, coronary heart disease, cardiac infarction, thromboangiitis obliterans, thrombotic phlebitis, Raynaud's disease, aorto-arteritis and hyperviscosity disease, etc. includes the following steps: using G75 chromatographic column to make chromatographic separation, taking Agkistrodon halys venin and dissolving it in phosphate buffer liquor, centrifugal sample-charging, column-charing, eluting with phosphate buffer liquor, collecting enzyme active peak, concentrating and freeze-drying, and using Sephadex-A25 chromatographic columnto make chromatographic separation, dissolving the chromatographic sample in Tris-HCl buffer liquor, soaking in gel with PH7.0, charging column, eluting with NaCl buffer liquor, collecting enzyme active peak, concentrating and freeze-drying to obtain dried powder.

Description

(1) Technical field: [0001] The invention relates to a thrombolytic injection with biologically active substances for treating cerebral thrombosis and a preparation method thereof. (two) background technology: [0002] With the progress of society and the improvement of living standards, there are more and more patients with thrombosis in society, and they tend to be younger. Several medicines for the treatment of thrombosis occurring at home and abroad are not very satisfactory. For example, Danshen injection, whose main ingredient is Danshen, has an effective rate of 80% in treating hyperlipidemia, and is cheap, but the effect is not significant. Donglingxobin treats cerebral thrombosis with an effective rate of 90%, but the price is 400 yuan / bottle higher, the effect is good, and it cannot be used for a long time. The main ingredients of Xiaoshuan Tongluo Tablets are Rhizoma Chuanxiong, Salvia Miltiorrhiza, Radix Astragali, etc., which play the role of promoting blood c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/43A61P9/10C12N9/48
Inventor 郝文学
Owner 郝文学
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