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Supersonic contrast medium and method for preparing supersonic contrast medium simultaneously as medicine and gene target carrier

An ultrasound contrast agent and carrier technology, applied in the field of clinical cardiovascular medicine, to achieve the effect of enhancing clarity and improving accuracy

Inactive Publication Date: 2005-07-06
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experimental papers on ultrasound contrast agents carrying anti-platelet antibodies have been published abroad, but there are few related studies in China

Method used

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  • Supersonic contrast medium and method for preparing supersonic contrast medium simultaneously as medicine and gene target carrier
  • Supersonic contrast medium and method for preparing supersonic contrast medium simultaneously as medicine and gene target carrier

Examples

Experimental program
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Effect test

preparation example Construction

[0063] The preparation method of ultrasound contrast agent with drug or gene targeting carrier is carried out according to the following steps:

[0064] 1) First prepare the above-mentioned ultrasound contrast agent for intravenous injection; the outer layer of the gas microbubbles in the ultrasound contrast agent for intravenous injection is distributed with surfactant alkylphenol polyoxyethylene (10) ether, sodium N-acyl glutamate And the molecular beam of sodium polycystine, so that the bubble wall of gas microbubbles has amine groups, amide groups, carboxyl groups, ether groups and sulfur bonds. Cell growth factor (bFGF) genes have an affinity effect and can be combined by secondary valence; the gas microbubbles are not only ultrasound contrast agents, but also gene and drug targeting carriers;

[0065] 2) At the same time, a variety of active functional groups in the bubble wall of gas microbubbles react with active functional groups in polylysine, VEGF, bFGF and other ge...

Embodiment

[0075] Air microbubbles are prepared by emulsion polymerization, and its formula is:

[0076] Cyanoacrylate 100 parts

[0077] Alkylphenol polyoxyethylene (10) ether 2.0 parts

[0078] Sodium N-acyl glutamate 1.5 parts

[0079] Sodium polycystine 1.5 parts

[0080] 4 parts peracetic acid

[0081] 300 parts of distilled water

[0082] Emulsion polymerization is carried out under strong stirring, and nitrogen gas is passed into the feed liquid, and the polymerization reaction is carried out at 65°C-78°C. The product obtained after the reaction is analyzed and tested by an electron microscope, and the particle size of the latex particles (ie, gas microbubbles) is basically 0.6 microns to 10.0 microns. The latex particles contain a certain amount of air such as nitrogen, and the polymer of the latex particles, namely polycyanoacrylate, is a biodegradable polymer. The outer layer of the latex particles is distributed with emulsifiers (i.e. surfactants) alkylphenol polyoxyethy...

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Abstract

The invention discloses an ultrasound contrast agent and a drug and a gene carrier targeted ultrasound contrast agent, which contain gas microbubbles with a diameter of 0.6 microns to 10 microns. Composed of body and surfactant, the surface is positively charged. Gas microbubbles are hollow, containing non-halocarbon gases such as air and nitrogen. Emulsion copolymerization of acrylic acid and its derivatives is carried out by emulsion copolymerization method. With the participation of surfactants, the surface tension between the gas phase and the liquid phase is adjusted so that the gas phase enters the polymer emulsion microbubbles. The gas microbubbles that are also drugs and gene carriers targeting ultrasound contrast agents contain polylysine or polyamino acids on the basis of the gas microbubbles of the above-mentioned ultrasound contrast agents, with active functional groups, and the groups are compatible with some gene plasmids The group coupling of the gas microbubbles couples the carboxyl groups of the gas microbubbles with GP II b / IIIa and other thrombus or anti-thrombotic or thrombolytic substances antibodies to make gas microbubbles carrying antibodies such as IIb / IIIa; the gas microbubble-wrapped anti The tumor drug is broken under the action of ultrasound, and the drug is released locally in the tumor.

Description

1. Technical field [0001] The invention belongs to clinical cardiovascular medicine, and relates to an ultrasonic contrast agent used in tumor medicine, chemistry and ultrasonic medicine of solid organs such as liver, kidney, spleen, prostate and breast. In particular, it relates to the preparation method of ultrasound contrast agent and ultrasound contrast agent combined with drug and gene targeting carrier. 2. Background technology [0002] 1. Current status of development of ultrasound contrast agents: [0003] The acoustic contrast agent is composed of gas-containing microbubbles similar to the size of red blood cells. After being injected into the vein, it can reach the parts that red blood cells can reach. Such as myocardium, liver, etc., produce strong contrast when detected by two-dimensional ultrasound equipment. Compared with other imaging techniques such as X-ray, it has the advantages of non-invasive, timely, bedside and cheap. The successful development of ul...

Claims

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Application Information

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IPC IPC(8): A61K47/32A61K49/18A61K49/22A61P35/00
Inventor 李丽君
Owner XI AN JIAOTONG UNIV
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