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Stationary liquid applied to frozen section, preparation method of stationary liquid and method for preparing frozen section based on stationary liquid

A frozen section and frozen section technology, which is applied to the field of frozen section fixative and its preparation, can solve the problems of unclear color development background, poor environmental protection of fixative reagents, low sensitivity, etc., and achieves improved sensitivity, reduced harm, and clear guiding significance. Effect

Pending Publication Date: 2022-07-26
SHAANXI MYBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the above-mentioned shortcomings of the prior art, the object of the present invention is to provide a fixative applied to frozen sections and a preparation method thereof and a method for preparing frozen sections based thereon, which can effectively solve the problems of poor environmental protection and sensitivity of fixatives in the prior art. Defective issues with low and unclear color rendering background

Method used

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  • Stationary liquid applied to frozen section, preparation method of stationary liquid and method for preparing frozen section based on stationary liquid
  • Stationary liquid applied to frozen section, preparation method of stationary liquid and method for preparing frozen section based on stationary liquid
  • Stationary liquid applied to frozen section, preparation method of stationary liquid and method for preparing frozen section based on stationary liquid

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preparation example Construction

[0043] The method for preparing frozen section of the present invention comprises the following steps:

[0044] Step 1 Acquisition of Organization

[0045] The animals were perfused with PBS, then the tissue was taken and cut into pieces;

[0046] The animals include commonly used experimental animals such as mice, rabbits, sheep, monkeys or cattle;

[0047] The tissue includes brain tissue, heart, kidney, liver, lung, sciatic nerve, optic nerve, intestine and tissue required for the experiment;

[0048] The size of the tissue block is 0.4cm 3 ~0.7cm 3 ;

[0049] Step 2 Embedding

[0050] The embedding is to place the tissue block obtained in step 1 in an embedding box containing a sufficient amount of OCT embedding compound to completely cover the tissue, put it into the freezing stage of the cryostat, and proceed after the tissue is completely frozen. slice;

[0051] The embedding can also be performed by placing the tissue block in a cassette containing a sufficient ...

Embodiment 1

[0069] Step 1 Acquisition of Organization

[0070] Adult rats were selected, anesthetized, the limbs of the rats were hardened, the abdominal cavity was opened, the apex of the heart was exposed, and PBS was perfused from the left apex for systemic circulation. medium, cut the tissue into 0.5cm 3 small pieces;

[0071] Step 2 Embedding

[0072] Coat a sufficient amount of OCT-embedding compound on the mouse brain tissue block so that it completely covers the tissue, put it on the freezing stage of a -20°C cryostat, and apply a thin layer of OCT to the surface of the specimen when the tissue is slightly whitish. Continue to freeze for 20min and slice;

[0073] Step 3 Slicing

[0074] Use a cryostat to cut the embedded mouse brain tissue block into slices with a thickness of 5um to 30um. When the cut tissue slices fall off the frozen block, place an adhesive glass slide to stick the tissue slices on the slide. on a glass slide;

[0075] Step 4 Fixation and staining of tiss...

Embodiment 2

[0085] Different from Example 1, the fixative of the tissue piece was fixed with 20 μg / mL Poria cocos fixative for 30 min. The experimental results are as figure 2 shown, from figure 2 It can be seen that the punctate positive signal can be clearly seen on the tissue pieces fixed by the Poria cocos solution, and the signal intensity is the same as that of the control group acetone ( Figure 9 shown) fixed results were similar, compared to the unfixed control group ( Figure 8 shown), the control group paraformaldehyde ( Figure 10 shown) fixed, control group ethanol ( Figure 11 shown) fixed signal strength.

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Abstract

The invention discloses stationary liquid applied to frozen sections, a preparation method of the stationary liquid and a method for preparing the frozen sections based on the stationary liquid, and belongs to the technical field of frozen section stationary liquid preparation. According to the invention, the traditional Chinese medicines astragalus membranaceus, poria cocos and codonopsis pilosula are used for fixing tissues for the first time, the formed fixing liquid can be obtained by preparing the traditional Chinese medicine powder by adopting a polyvinyl alcohol solution, and the use of an organic solvent is completely avoided, so that the harm to experimenters and the environment is reduced. Meanwhile, compared with the stationary liquid in the prior art, the stationary liquid disclosed by the invention can obtain the same signal or a signal higher than that of the conventional stationary liquid, so that the sensitivity of a to-be-detected sample is improved to a certain extent, and a clearer guiding significance is provided for clinical diagnosis and treatment.

Description

technical field [0001] The invention belongs to the technical field of frozen section fixative preparation, and in particular relates to a fixative applied to frozen sections, a preparation method thereof, and a method for preparing frozen sections based thereon. Background technique [0002] Frozen sectioning is a technique in which tissue is rapidly cooled to a certain hardness under low temperature conditions, and then sectioned. This method is the most commonly used sectioning method in immunohistochemical staining. The advantage of frozen section is that it can better preserve the immune activity of various antigens, especially cell surface antigens (membrane antigens), and the staining results obtained by frozen section will be better. [0003] The fixation of the section is one of the factors affecting the quality of frozen sections. The existing fixative reagents for frozen sections mainly include the following categories: acetone, formaldehyde, ethanol and acetic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/307
Inventor 闫亚平马佳乐党嘉萌王亚兰封雪李科
Owner SHAANXI MYBIOTECH CO LTD
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