Longitudinal relaxation rate measuring system and measuring method using same

Abstract

The invention provides a longitudinal relaxation rate measurement system and a measurement method using the system. The system comprises a pump light unit, a confocal microscopic collection unit, a displacement unit, a microwave radiation unit, a static magnetic field unit, a diamond probe containing a nitrogen-vacancy color center and a computer control processing unit. The measurement method using the system comprises the following steps: S1, connecting a first biomolecule to hydrogel in a biochemical reaction manner; s2, cleaning the hydrogel after the operation in the step S1, dropwise adding second and third biomolecules with different spin labels onto the hydrogel for reaction, and then fully cleaning to obtain a to-be-detected sample of interaction of the first biomolecule, the second biomolecule and the third biomolecule; and S3, carrying out longitudinal relaxation rate measurement on the to-be-measured sample. The system can be used for measuring the interaction of biomolecules, and has the advantages of stable signal, high signal-to-noise ratio, capability of simultaneously detecting multi-channel biological samples and the like.

Description

technical field [0001] The invention relates to the field of biological sample detection, in particular, to a longitudinal relaxation rate measurement system and a measurement method using the system. The system is implemented based on the optical properties of diamond nitrogen-vacancy color centers, and is used for nitrogen- The longitudinal relaxation rate of the vacancy color center is measured, and biomolecular interactions can be measured using this system. Background technique [0002] The conformational changes of biomolecules themselves and the biomolecular interactions affected by them are extremely basic and important processes in biological life activities. Therefore, the detection of the above-mentioned processes has become a very important research direction in life sciences. [0003] Among many methods for detecting molecular interactions, fluorescent biomarker detection is a widely used method. It labels the target protein molecules with fluorescent molecule...

AI Technical Summary

Problems solved by technology

However, this method still faces two problems. One is that the signal-to-noise ratio is low due to the limited protein connection density, which affects subsequent measurement and imaging; the other is that this method cannot differentiate the mixed reaction of multiple target proteins. Multi-channel detection to tell which protein is responsible for the process

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