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Dental bone material for in-vitro collagen demineralization

A bone and demineralization technology, applied in the field of biomedical engineering, can solve the problem of low mechanical strength, achieve the effect of short processing time, good mechanical strength, and promote cell adhesion

Pending Publication Date: 2022-07-22
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In related technologies, small molecule demineralizing agents such as EDTA and hydrochloric acid are used for non-selective demineralization treatment of dentin matrix, and the minerals inside and outside the collagen fibers are removed at the same time, so there is a technical defect of low mechanical strength

Method used

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  • Dental bone material for in-vitro collagen demineralization
  • Dental bone material for in-vitro collagen demineralization
  • Dental bone material for in-vitro collagen demineralization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] S1. Take fresh human teeth, ultrasonically clean them with deionized water for 3 times, filter the water, add disinfectant (75% ethanol), soak for 15 minutes at room temperature, filter out the solution, soak in sterile deionized water for 1 hour , ultrasonic cleaning for 5 minutes, repeat the above soaking ultrasonic cleaning 3 times;

[0040] S2. Use mechanical methods to remove the enamel of the tooth, open the pulp, and extract the pulp tissue to obtain a dentin matrix-based material A;

[0041] S3. Use an electric grinder to pulverize material A, and collect dentin powder with a particle size of 0.2-0.8 mm to obtain material B;

[0042] S4. Soak material B in sterile phosphate buffered saline (PBS) for ultrasonic cleaning, replace the liquid every 10 minutes for a total of 30 minutes of cleaning, and remove the cleaning solution to obtain material C;

[0043] S5. Add material C into a 15% wt PAAN (Mw=225kDa) sodium polyacrylate aqueous solution, stir for 12 hours,...

Embodiment 2

[0047]S1. Take fresh human teeth, ultrasonically clean them with deionized water for 3 times, filter the water, add disinfectant (75% ethanol), soak for 15 minutes at room temperature, filter out the solution, soak in sterile deionized water for 1 hour , ultrasonic cleaning for 5 minutes, repeat the above soaking ultrasonic cleaning 3 times;

[0048] S2. Use mechanical methods to remove the enamel of the tooth, open the pulp, and extract the pulp tissue to obtain a dentin matrix-based material A;

[0049] S3. Use a slow-speed saw to cut material A to obtain a 2×2×1 mm cube-shaped dentin sheet material B;

[0050] S4. Soak material B in sterile phosphate buffered saline (PBS) for ultrasonic cleaning, replace the liquid every 10 minutes for a total of 30 minutes of cleaning, and remove the cleaning solution to obtain material C;

[0051] S5. Add material C into 15% wt PAAN (Mw=225kDa) sodium polyacrylate aqueous solution, stir for 24 hours, remove the solution, then inject ster...

Embodiment 3

[0055] The sodium polyacrylate involved in S5 in Example 1 was replaced with carboxymethyl chitosan, and the others were the same as in Example 1.

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Abstract

The invention discloses a dentale material with extracorporeal collagen demineralization. The dentale material is based on the size exclusion effect of collagenous fibers, a macromolecular chelating agent is combined with calcium outside the dentin collagenous fibers, mineral crystals outside the collagenous fibers are removed, and the external demineralization dentale material with a unique nano structure and high mechanical strength is obtained. The extrafibrous demineralized dental bone material shows a unique nano-structure formed by endomineralized collagen fibers, has excellent mechanical strength and biocompatibility, can effectively promote adhesion and proliferation of cells, support cell growth, promote osteogenic differentiation of stem cells, and has a good application prospect. And pores left after the mineral substances outside the fibers are removed are beneficial to tissue vascularization and nutrient substance transportation. Therefore, the collagen in-vitro demineralized dental bone material can be used as a tissue engineering scaffold material to be applied to bone tissue regeneration, and has a good biomedical application prospect.

Description

technical field [0001] The present application relates to the technical field of biomedical engineering, and in particular, to a dental bone graft material with extra-collagen demineralization. Background technique [0002] There are various choices of bone graft materials. Among them, autologous bone graft has no immune response, has shorter healing time and faster osseointegration rate, and is the gold standard for bone graft surgery (Piattelli, A.; Degidi, M. ; DiStefano, D.A.; Rubini, C.; Fioroni, M.; Strocchi, R., Microvessel density in alveolarridge regeneration with autologous and alloplastic bone. Implant Dent 2002, 11(4), 370-5). However, due to the need to open a second surgical area, autologous bone transplantation has many complications, high self-reported pain rate, and high cost (Chavda, S.; Levin, L., Human Studies of Vertical and Horizontal Alveolar Ridge Augmentation Comparing Different Types of BoneGraft Materials: A Systematic Review. J Oral Implantol 201...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/56
CPCA61L27/3604A61L27/3641A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2430/12
Inventor 杨宏业黄翠吴小艺
Owner WUHAN UNIV
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