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Hansenula anomala MP261 capable of inhibiting phytopathogen and application of hansenula anomala MP261

A technology of Hansenula sporogenes and MP261, which is applied in the field of microorganisms, can solve problems such as environmental pollution and drug residues, and achieve broad-spectrum antibacterial properties and good market application prospects

Active Publication Date: 2022-07-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, chemical control methods are mainly used to control plant diseases, but the long-term and large-scale use of pesticides will cause environmental pollution and drug residue problems.

Method used

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  • Hansenula anomala MP261 capable of inhibiting phytopathogen and application of hansenula anomala MP261
  • Hansenula anomala MP261 capable of inhibiting phytopathogen and application of hansenula anomala MP261
  • Hansenula anomala MP261 capable of inhibiting phytopathogen and application of hansenula anomala MP261

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Isolation and screening of strains

[0042] In September 2019, samples of leaves, roots and petals of different plants were collected in Laoshan, Qingdao, and the lesions and necrotic parts were removed and cut into pieces for use. After cutting the samples, they were placed in YPD liquid medium containing a final concentration of 0.01% chloramphenicol. (1% YeastExtract, 2% Peptone, 2% Dextrose), 28 °C, 180 rpm shaking culture for 2 days; take the above culture medium diluted with sterile water, spread on YPD plate for separation, and initially observe strains after 28 °C culture for 2 days. Pick a suitable single colony for streak separation again to obtain a pure cultured strain.

[0043] Pick a single colony of strains, use the plate confrontation growth method to inoculate a single colony and a variety of plant pathogenic bacteria on a potato dextrose agar (PDA) plate (200 grams of potato, wash and cut into pieces, add appropriate water and boil for 20 to 30 minu...

Embodiment 2

[0066] Dip the Ralstonia solanacearum solution with a sterile cotton swab, spread it on the surface of the plate, and then inoculate the MP261 strain in the center of the plate. The petri dish was placed upside down in an incubator (26-28°C) for 1-2 days, and the bacteriostatic effect of MP261 strain on Ralstonia solanacearum was determined.

[0067] Test results such as Figure 14 As shown in Table 2, it was found that an obvious inhibition zone appeared around the colony of the MP261 strain, indicating that the MP261 strain also had a significant inhibitory effect on the growth of Ralstonia solanacearum.

[0068] Table 2. Inhibitory effect of MP261 strain on R. solanacearum

[0069]

Embodiment 3

[0071] The volatile gas generated by MP261 was collected by a 20ml headspace vial, and the components of the volatile gas were tested by solid phase extraction and gas-mass spectrometry GC-MS instruments. Solid phase extraction: SIGMA-ALDRICH 57329-U (DVB / CAR / FDMS); GC-MS instrument: TSQ series 8000; chromatographic column: Thermo TG-5SILMS, capillary column (30m×0.25mm×0.25μm). Headspace extraction was performed for 1 hour at 40°C. The injection temperature was 250°C, the carrier gas: He, and the flow rate was 1.3 ml / min. Column temperature heating program: hold at 40 °C for 2 min, then increase to 100 at 3 °C / min, hold for 0.1 min; then increase to 240 °C at 20 °C / min, hold for 5 min. Chromatograms were recorded in full scan mode, m / z 40-400 amu, and the ion source temperature was set to 230 °C. The obtained substances were automatically searched by computer and matched with the NIST Library.

[0072] The experimental results show that MP261 can produce a variety of volat...

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Abstract

The invention discloses a hansenula anomala MP261 strain for inhibiting phytopathogen and an application of the hansenula anomala MP261 strain. The vineyard hansenula polymorpha MP261 is classified and named as the vineyard hansenula polymorpha, the vineyard hansenula polymorpha MP261 is preserved in the China Center for Type Culture Collection, and the preservation number of the vineyard hansenula polymorpha MP261 is CCTCC (China Center for Type Culture Collection) NO: M 2022262. The vineyard hansenula polymorpha MP261 has a remarkable inhibition effect on a plurality of phytopathogens such as ralstonia solanacearum, phytophthora nicotianae, phytophthora capsici, botrytis cinerea, alternaria alternata, fusarium graminearum, fusarium oxysporum and corynespora cassiicola. Compared with chemical prevention and control which is mostly used at present, the method for performing biological prevention and control by utilizing the vineyard hansenula polymorpha MP261 has the advantages of being environment-friendly, non-toxic, pollution-free, not easy to generate drug resistance and the like, and has a good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a vineyard Hansenula spp. MP261 which inhibits plant pathogenic bacteria and its application. Background technique [0002] Plant diseases caused by fungi, oomycetes and bacteria are widespread worldwide, affecting the yield and quality of crops and commercial crops. In order to control these plant diseases, people use chemical control, agricultural control and biological control to inhibit the growth of plant pathogens. Although chemical control is the most commonly used control method, it has the characteristics of high efficiency, but it has disadvantages such as causing pathogenic bacteria to develop drug resistance, pesticide residues polluting the environment and endangering human health. [0003] Biological control mainly uses bio-control bacteria, bio-control fungi, bio-control actinomycetes, and plant antagonists for control, which is environmentally ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/62C12P7/22A01N63/32A01P1/00A01P3/00C12R1/645
CPCC12N1/16C12P7/62C12P7/22A01N63/32
Inventor 梁文星申恒王光远刘子瑜田俊婕
Owner QINGDAO AGRI UNIV
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