Functional sponge for promoting wound healing and reducing wound scar formation and preparation method and application thereof

A functional, sponge technology, applied in the field of biomedical engineering, can solve the problems of scars, scarless healing, scars without effective treatment and intervention methods, etc., to achieve high water absorption, reduce fibrosis, and low cost effects

Pending Publication Date: 2022-07-12
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The treatment of scars caused by excessive repair of skin wounds is a worldwide problem. Currently, there is no effective treatment and intervention for scars
In adults, wound healing aims to rapidly restore the skin's barrier function, but results in scarring
Although there is currently no therapy to support scarless wound healing in adult skin, since the discovery of scarless healing in mammalian embryos, it is suggested that the wound environment is one of the most basic guarantees for scarless healing after skin trauma. If scarless healing is simulated The embryonic physiological environment, the possibility of scarless healing after skin trauma

Method used

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  • Functional sponge for promoting wound healing and reducing wound scar formation and preparation method and application thereof
  • Functional sponge for promoting wound healing and reducing wound scar formation and preparation method and application thereof
  • Functional sponge for promoting wound healing and reducing wound scar formation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Optimization of the preparation process of HA-CE sponge

[0056] 1. Preparation of COS-Eu complexes:

[0057] First, 1.2 g of COS was completely dissolved in 100 mL of deionized water. The pH of the COS solution was adjusted to 5.5 with 1M hydrochloric acid. Put Eu (NO 3 ) 3 Stir continuously with the above COS solution. The obtained samples were collected by high-speed centrifugation at 10,000 rpm, frozen in liquid nitrogen, and then transferred to a freeze dryer for vacuum drying to obtain COS-Eu complexes.

[0058] Among them, Eu(NO 3 ) 3 The addition amount of the solution was calculated according to its final concentration in the system as 0.01mol / L, 0.02mol / L and 0.03mol / L, respectively, the reaction temperature was set to 25, 50, 70 and 100°C, and the stirring time was set to 5 and 24h. According to the yield and SEM morphology of the obtained samples, the final sample preparation conditions were selected as 0.01 mol / L and continuous stirring at...

Embodiment 2

[0062] Example 2: Preparation and characterization of HA-CE sponge

[0063] Step (1) Preparation of COS-Eu complex:

[0064] First, 1.2 g of COS was completely dissolved in 100 mL of deionized water. The pH of the COS solution was adjusted to 5.5 with 1M hydrochloric acid. 0.01mol / L Eu(NO 3 ) 3 Stir continuously with the above COS solution at 50 °C for 5 h. The obtained samples were collected, frozen with liquid nitrogen, and then freeze-dried to obtain the COS-Eu complex.

[0065] Step (2) Preparation of HA-CE sponge:

[0066] Weigh 3 g of hyaluronic acid and dissolve it in 100 mL of deionized water with pH=5, and stir with a stirrer at low speed for 24 h to prepare a hyaluronic acid solution. EDC and NHS with a final concentration of 50 mmol / L were added, respectively, and the reaction was stirred at room temperature in the dark for 15 min to activate the carboxyl group of hyaluronic acid. Then, 1.0 g of COS-Eu complex was added to the reaction system, and the reactio...

Embodiment 3

[0083] Example 3: Performance analysis of HA-CE sponge

[0084] 1. Analysis of antioxidant properties of HA-CE sponge

[0085] 1,1-Diphenyl-2-picrylhydrazine radical (DPPH) is widely used in the quantitative determination of the antioxidant capacity of samples. The DPPH radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to its single electron pairing, and the lighter the color, the lower the absorbance value.

[0086] The sponge was added to the DPPH solution (0.1 mM, 4 mL, using methanol as solvent), and after shaking in the dark for 30 min, the color changes in each group were recorded by photographing, and the supernatant was collected and transferred to a glass cuvette. The absorbance of the sample supernatant at 517 nm was recorded using a UV spectrophotometer, and each group of samples was tested 3 times. Finally, the DPPH scavenging rate...

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Abstract

The invention discloses functional sponge for promoting wound healing and reducing wound scar formation as well as a preparation method and application of the functional sponge. The method comprises the following steps: dissolving hyaluronic acid in water with the pH value of 4.5-5.5, and stirring to obtain a hyaluronic acid glue solution; adding EDC and NHS, carrying out a stirring reaction in a dark place, then adding the chitosan oligosaccharide-europium complex, continuing the reaction, injecting a reaction solution into a mold, and continuing the reaction in a shaking table to obtain a cross-linked hyaluronic acid gel; freezing and drying to obtain the hyaluronic acid / chitosan oligosaccharide-europium functional sponge, namely the functional sponge for promoting wound healing and reducing wound scar formation. The method is mild in experimental condition and simple and convenient in step, the chitosan oligosaccharide-europium complex is used as a cross-linking agent, chitosan oligosaccharide with an anti-inflammatory effect and europium element with a vascularization promoting function are introduced at the same time, and the obtained HA-CE is obvious in anti-oxidation, anti-inflammatory, vascularization promoting and fibrosis reducing effects and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, and in particular relates to a functional sponge for promoting wound healing and reducing the formation of scars on wound surfaces, and a preparation method and application thereof. Background technique [0002] For many skin damage diseases, skin burns are a common type of damage, mainly caused by high temperature, or skin damage caused by radiation, radiation, electricity, friction or exposure to chemicals, causing varying degrees of skin function and appearance. damage, which may be life-threatening. Although early burn wound excision and skin grafting can significantly improve the outcome of severe burn patients, however, slow wound healing, infection, pain and scarring remain major problems in burn research and clinical management, with the greatest challenge being the prevention of skin scarring and treatment. The incidence of scarring after skin burns is about 70%, which c...

Claims

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Application Information

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IPC IPC(8): A61L15/28A61L15/18A61L15/20A61L15/42A61L15/44
CPCA61L15/28A61L15/18A61L15/20A61L15/42A61L15/425A61L15/44A61L2300/412A61L2300/41A61L2300/236A61L2300/102C08L5/08
Inventor 彭素芬黄秀红刘宗华
Owner JINAN UNIVERSITY
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