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Responsive chitosan microsphere/cellulose hydrogel drug-loading composite membrane and preparation thereof

A technology of cellulose hydrogel and chitosan microspheres, which is applied in the field of biomedicine to achieve the effects of avoiding damage, prolonging the action time, and obvious antibacterial effect

Active Publication Date: 2018-11-27
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no research or report on the implantable reduction-responsive hydrogel membrane composed of drug-loaded chitosan microspheres and drug-loaded carboxymethylcellulose hydrogel to achieve targeted release of anticancer drugs and slow release of antibacterial drugs, both anticancer and antibacterial functions

Method used

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  • Responsive chitosan microsphere/cellulose hydrogel drug-loading composite membrane and preparation thereof
  • Responsive chitosan microsphere/cellulose hydrogel drug-loading composite membrane and preparation thereof
  • Responsive chitosan microsphere/cellulose hydrogel drug-loading composite membrane and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Preparation of chitosan microspheres loaded with antibacterial drugs. Weigh 0.12g of chitosan, add it into 6mL of 1% acetic acid solution, stir until the chitosan is completely dissolved, then add 0.04g of tetracycline hydrochloride, stir until completely dissolved, add 1mL of Tween-80, ultrasonic 1min, as water Phase: Measure 24 mL of liquid paraffin, add 150 μL of 25% glutaraldehyde solution and 1 mL of Span-80, and sonicate for 2 min, as the oil phase. Drop the water phase into the oil phase, keep stirring for 30 minutes, then drop 50 μL of 25% glutaraldehyde solution, keep stirring at 40°C for 2 hours, then centrifuge, wash the precipitate with petroleum ether three times, and vacuum freeze-dry for 24 hours. Chitosan microspheres loaded with tetracycline hydrochloride were obtained.

[0021] (2) Preparation of drug-loaded composite film. Weigh 0.1 g of carboxymethyl cellulose and dissolve it in 10 mL of water to obtain a carboxymethyl cellulose solution with a...

Embodiment 2

[0023] (1) Weigh 0.24g of chitosan, add it to 12mL of 1% acetic acid solution, stir until the chitosan is completely dissolved, then add 0.08g of tetracycline hydrochloride, stir until completely dissolved, add 1mL of Tween-80, and sonicate for 1min , as the water phase; measure 48 mL of liquid paraffin, add 300 μL of 25% glutaraldehyde solution and 1 mL of Span-80, and sonicate for 1 min, as the oil phase. Drop the water phase into the oil phase, keep stirring for 35 minutes, then drop 100 μL of 25% glutaraldehyde solution, keep stirring at 40°C for 2 hours, then centrifuge, wash the precipitate with petroleum ether three times, and vacuum freeze-dry for 24 hours. Chitosan microspheres loaded with tetracycline hydrochloride were obtained.

[0024] (2) Preparation of drug-loaded composite film. Weigh 0.15 g of carboxymethyl cellulose and dissolve it in 10 mL of water to obtain a carboxymethyl cellulose solution with a concentration of 1.5%. Add 0.2 g of 1-(3-dimethylaminopro...

Embodiment 3

[0026] (1) Weigh 0.36g of chitosan, add it to 18mL of 1% acetic acid solution, stir until the chitosan is completely dissolved, then add 0.08g of tetracycline hydrochloride, stir until completely dissolved, add 2mL of Tween-80, and sonicate for 1min , as the water phase; measure 72 mL of liquid paraffin, add 450 μL of 25% glutaraldehyde solution and 1 mL of Span-80, and sonicate for 1.5 min, as the oil phase. Drop the water phase into the oil phase, keep stirring for 40 minutes, then drop 150 μL of 25% glutaraldehyde solution, keep stirring at 40°C for 2 hours, then centrifuge, wash the precipitate three times with petroleum ether, and vacuum freeze-dry for 24 hours. Chitosan microspheres loaded with tetracycline hydrochloride were obtained.

[0027] (2) Preparation of drug-loaded composite film. Weigh 0.2 g of carboxymethyl cellulose and dissolve it in 10 mL of water to obtain a 2% carboxymethyl cellulose solution. Add 0.3 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide ...

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Abstract

The invention discloses a responsive chitosan microsphere / cellulose hydrogel drug-loading composite membrane and preparation thereof. The preparation includes: preparing chitosan microspheres that load tetracycline hydrochloride which is an antibacterial drug; dispersing the prepared chitosan microspheres and 5-fluorouracil evenly in three-dimensional network structure formed by crosslinking carboxymethyl cellulose and cystamine dihydrochloride, wherein 5-fluorouracil is an anticancer drug. The responsive chitosan microsphere / cellulose hydrogel drug-loading composite membrane has good biocompatibility and reduction response, can release tetracycline hydrochloride and 5-fluorouracil in controlled manner in cancer tissue areas, and has antibacterial and anticancer functions.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the preparation and product of a responsive chitosan microsphere / cellulose hydrogel drug-loaded composite film. Background technique [0002] Cancer is a leading cause of human death, currently claiming more than 8 million lives worldwide each year. Common cancer treatment methods are mainly divided into surgical resection of cancer tissue and radiotherapy and chemotherapy. Surgical resection of cancer tissue may cause infection on the one hand, and antibacterial drugs are usually used for treatment. Drugs mainly act on the human body through free diffusion, so it is difficult to control the amount of drugs, and high concentrations of drugs will affect normal tissues. and organ side effects. More importantly, surgery may cause tissue damage and residual small lesions, and tumor cells will enter the human body's circulatory system, which will lead to recurrence and metastasis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/38A61K47/36A61K31/65A61K31/513A61P35/00A61P31/04
CPCA61K9/0024A61K9/7007A61K31/513A61K31/65A61K47/36A61K47/38A61P31/04A61P35/00A61K2300/00
Inventor 王芳张茜邵伟
Owner NANJING FORESTRY UNIV
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