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Method for generating alpha-glucan with specific molecular weight by utilizing three-aqueous-phase system to spontaneously regulate and control hydrolysis and application of alpha-glucan

A dextran and molecular weight technology, applied in the field of α-glucan, can solve problems such as the inability to control the molecular weight of α-glucan, and achieve the effects of mild conditions, improved economic benefits, and simple recovery

Pending Publication Date: 2022-07-01
GUANGDONG ACAD OF SCI INST OF BIOENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN103571806 proposes to extract dextran sucrase by two-phase aqueous phase, wherein the target object is a synthetase, and the catalytic direction is opposite, and secondly, exogenous α-glucan needs to be introduced, and the control of the final α-glucan molecular weight cannot be achieved
Since α-glucan itself is a common component of aqueous phase extraction, it is generally not considered to participate in the aqueous phase extraction and reaction system of the corresponding α-glucanase, and it has not been seen in the aqueous phase extraction system so far. Method for rapidly stopping reaction and recovering free α-glucanase during hydrolysis of α-glucan in medium

Method used

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  • Method for generating alpha-glucan with specific molecular weight by utilizing three-aqueous-phase system to spontaneously regulate and control hydrolysis and application of alpha-glucan
  • Method for generating alpha-glucan with specific molecular weight by utilizing three-aqueous-phase system to spontaneously regulate and control hydrolysis and application of alpha-glucan

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Experimental program
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Effect test

Embodiment 1

[0028] Example 1: The method for spontaneously regulating and hydrolyzing α-glucan with a weight-average molecular weight of about 100 kDa by using a three-water phase system

[0029] Reagents such as polyethylene glycol, polypropylene glycol, potassium dihydrogen phosphate, magnesium sulfate, acetic acid, and sodium acetate used in this example are all analytically pure.

[0030] The α-glucanase is derived from the Pichia pastoris engineering bacteria provided by the China Type Culture Collection, the preservation number is CCTCC No: M2013096; the Pichia pastoris engineering bacteria and the α-glucanase The preparation method of glucanase has been disclosed in patent 2013102877278. Alpha-glucan was prepared by single batch fermentation in shake flasks from Leuconostoc mesenteroides with a deposit number of GDMCC1.473 (Leuconostoc mesenteroides was purchased from the Guangdong Provincial Microbial Culture Collection Center), and the specific fermentation process was a 5L ferme...

Embodiment 2

[0037] Embodiment 2: Utilize the method for spontaneously regulating and hydrolyzing the α-glucan with a weight-average molecular weight of about 500kDa by using the three-water system

[0038] Reagents such as polyethylene glycol, polypropylene glycol, potassium dihydrogen phosphate, sodium chloride, ammonium sulfate, sodium sulfate, magnesium sulfate, acetic acid, and sodium acetate used in this example are all analytically pure.

[0039] The glucanase is derived from the Pichia pastoris engineering bacteria provided by the China Type Culture Collection, the preservation number is CCTCC No: M2013096; the Pichia pastoris engineering bacteria and α-glucans with the preservation number CCTCC NO: 2013096 The preparation method of carbohydrase has been disclosed in patent 2013102877278. The α-glucan was purchased from Leuconostoc mesenteroides with the deposit number GDMCC1.473 (from the Guangdong Provincial Microbial Culture Collection). The specific fermentation process is: 5L...

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Abstract

The invention discloses a method for generating alpha-glucan with specific molecular weight through spontaneous regulation and hydrolysis of a three-aqueous-phase system and application of the method, and belongs to the technical field of enzymatic reaction regulation. Glucan is not only a polymer capable of forming a phase body, but also the molecular weight of glucan is a key factor influencing a phase forming condition, and the final target molecular weight of the enzymolysis reaction falls on a phase splitting point by adjusting the phase body composition of other high polymers, so that the purpose of spontaneously stopping the reaction is achieved. Meanwhile, the high partition coefficient of the alpha-glucanase in part of the polymer phase is utilized to quickly recover the zymoprotein from the reaction phase and stop the enzymolysis reaction, and after the low-molecular-weight alpha-glucan is separated, the zymoprotein is recovered again through a concentrated salt solution.

Description

technical field [0001] The invention belongs to the technical field of enzymatic reaction regulation, and in particular relates to a method for generating α-glucan with a specific molecular weight by spontaneous regulation and hydrolysis of a three-water phase system and its application Background technique [0002] The current production process of low molecular weight α-glucan in the food or pharmaceutical industry consists of the following steps: fermentation-ethanol precipitation-hydrolysis-separation and purification-lyophilization. Among them, the hydrolysis methods generally include acid hydrolysis and enzymatic hydrolysis, and many improvement schemes focus on the combination of hydrolysis and other processes. For example, in CN101205257, high molecular weight α-glucan is obtained by fermentation and then acid hydrolyzed to a specified molecular weight. The steps of membrane separation, ion exchange resin desalination and activated carbon decolorization obtain the fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08
CPCC12P19/08
Inventor 黎志德刘桂云梁达奉柳颖张九花
Owner GUANGDONG ACAD OF SCI INST OF BIOENGINEERING
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