Bacillus subtilis engineering bacterium for expressing MccJ25 as well as construction method and application of bacillus subtilis engineering bacterium

Abstract

The invention discloses a bacillus subtilis engineering bacterium for expressing MccJ25 as well as a construction method and application of the bacillus subtilis engineering bacterium. The engineering bacterium is constructed by cloning a mcjABCD sequence which is modified by lasso peptide MccJ25 to bacillus subtilis. Wherein the sequence of the mcjABCD is as shown in SEQ ID NO. 1. The constructed bacillus subtilis engineering bacteria can successfully express the MccJ25, and the bacillus subtilis belongs to food-grade probiotics and can avoid the influence of endotoxin, so that the bacillus subtilis engineering bacteria can be used as probiotics for producing the MccJ25; meanwhile, the engineering bacterium also has a relatively strong inhibition effect on salmonella enteritidis and the like, and can be used in the aspect of antibacterial products so as to improve the application value of MccJ25.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, protein expression and function verification, and particularly relates to a Bacillus subtilis engineering bacterium expressing MccJ25 and a construction method and application thereof. Background technique [0002] MccJ25 is a bacteriocin class I antibacterial small peptide found in Escherichia coli. Its size is composed of 21 amino acids. Its lariat structure is a β hairpin structure formed by the 9th to 21st amino acids of the N-terminal. It runs through the 1st to 8th amino acids. It is formed by a cyclic structure formed by a single amino acid and maintains stability through covalent action. Therefore, mature MccJ25 has a certain tolerance to extreme environments. Usually, MccJ25 inhibits bacterial transcription by blocking the transport of bacterial RNA polymerase, or by participating in the inhibition of bacterial membrane respiratory chain, thereby killing sensitive bacteria or...

AI Technical Summary

Problems solved by technology

[0003] At present, most of the secreted expression and actual production of MccJ25 use Gram-negative bacteria such as Enterobacter as the expression strain, and Enterobacter cannot avoid the influence of endotoxin

For Gram-positive bacteria, due to the restriction of the expression system of the bacteria itself, very few people use Gram-positive bacteria to express MccJ25

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