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Bacillus licheniformis for straw degradation and application thereof

A technology of Bacillus licheniformis and straw, which is applied to microorganism-based methods, bacteria, fungi, etc., can solve the problems of environmental pollution and low utilization rate of straw, achieve strong tolerance, improve maturity and fertilizer efficiency, and improve maturity. degree of effect

Active Publication Date: 2022-05-13
山东蔚蓝生物科技有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As an excellent biomass energy, straw has great application prospects. However, due to the influence of the composition of straw itself, the utilization rate of straw is not high, and it even causes huge environmental pollution problems.

Method used

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  • Bacillus licheniformis for straw degradation and application thereof
  • Bacillus licheniformis for straw degradation and application thereof
  • Bacillus licheniformis for straw degradation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 strain screening

[0034] 1.1 Sample:

[0035] Collected from broiler waste in Penglai, Yantai.

[0036] 1.2 Initial screening of cellulose-degrading strains:

[0037] Take 1g of chicken manure and add it to a culture bottle containing 99 mL of sterile water, and add a few glass beads to make a suspension; take 1 mL of the suspension and put it into a culture bottle containing 50 mL of enriched medium, seal it , placed in a biochemical incubator at 30°C for static culture for 3-7 days. Spread the enriched culture on the separation medium, culture at 30°C for 2 days, pick out a single colony for purification, and then continue to purify on the separation medium for 2 to 3 times.

[0038] Finally, the applicant successfully isolated three strains with cellulose-degrading ability by using the enrichment medium and separation medium with carboxymethylcellulose sodium (CMC-Na) as the sole carbon source, and named them VB372, VB375, VB376.

[0039] 1.3 Re-scree...

Embodiment 2

[0056] The identification of embodiment 2 VB376 bacterial strain

[0057] 2.1 Identification of colony morphology

[0058] The colony morphology of the VB376 strain is as follows figure 1 As shown, the colony is off-white, 3-5mm in diameter, with rough edges and a bulge in the middle. Electron microscope observation results such as figure 2 As shown, the VB376 bacterium is a short straight rod-shaped, Gram-positive bacterium that can produce spores, the sporangia do not expand, and the cells are solitary.

[0059] 2.2 16S rDNA molecular identification

[0060] The genome of the VB376 strain was extracted using a kit. Then using the genome as a template, the 16S rDNA was amplified using specific primers. The amplified PCR products were detected by 1% agarose gel electrophoresis and sent to a sequencing company for sequencing.

[0061] Sequencing results showed that the sequence of the PCR amplification product was SEQ ID NO:1. Through the BLAST comparison of the sequen...

Embodiment 3

[0073] Example 3 Evaluation of Enzyme Production Ability and Low Temperature Resistance of Bacillus licheniformis VB376

[0074] 1. Preparation of bacteria solution

[0075] The activated Bacillus licheniformis VB376 was inoculated into LB liquid medium, cultivated at 37°C and 220rpm for 14h, and the viable count was 10 8 -10 9 CFU / ml bacterial solution, adjusted to 10 with sterile LB medium 8 CFU / ml.

[0076] 2. Evaluation of cellulase production ability

[0077] Spot the Bacillus licheniformis VB376 bacteria solution on the separation medium, and incubate at 37°C for 48 hours. A transparent circle can be seen around the Bacillus licheniformis VB376, and the diameter of the transparent circle is 18 mm. This shows that Bacillus licheniformis VB376 can produce certain cellulase.

[0078] 3. Evaluation of the ability to produce endoglucanase

[0079]The Bacillus licheniformis VB376 bacterial liquid was centrifuged at 4°C and 12000 rpm for 10 min, and the enzyme activity of...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides bacillus licheniformis for straw degradation and application of the bacillus licheniformis. The preservation number of the bacillus licheniformis is CCTCC NO: M2019438, and the bacillus licheniformis can efficiently degrade straw fibers, is widely applied to straw returning and composting of organic materials, and remarkably improves the decomposition degree and fertilizer efficiency of compost. In addition, the strain can effectively promote softening of straw and is widely applied to the field of papermaking.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a novel bacillus licheniformis and its application in straw organic fertilizer composting production, straw softening and straw returning to the field. Background technique [0002] Straw refers to the general term for the stems and leaves of mature crops. In my country, it generally refers to the remaining parts of grasses and leguminous plants such as corn, rice, sorghum, and sugarcane after harvesting seeds. Crop straw is rich in nitrogen, phosphorus, potassium and organic matter nutrients. It is a traditional source of organic fertilizer in China, but it needs to be fermented and decomposed by microorganisms before it is suitable for field use. The decomposing process of crop straw is that microorganisms decompose organic matter in straw into CO under aerobic or anaerobic conditions. 2 、H 2 O, NH 3 and humus processes. Due to the complex composi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C05F11/08C05F17/20C12R1/10C12R1/66
CPCC12N1/20C12N1/14C12N1/16C05F11/08C05F17/20Y02W30/40
Inventor 凌红丽焦绪勇苑伟伟周英俊陈琳赵凯张春玉梁莉王宝琛
Owner 山东蔚蓝生物科技有限公司
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