Microreactor array chip for nucleic acid synthesis and preparation method thereof

Abstract

The invention provides a nucleic acid synthesis-oriented micro-reactor array chip and a preparation method thereof, the micro-reactor array chip with a high surface area is constructed by ink-jet printing of a nanoparticle droplet array on the surface of a synthetic chip and combination of a three-phase contact line regulation and control method and a high-temperature sintering technology at the same time, and the nucleic acid synthesis-oriented micro-reactor array chip is prepared. High-flux and high-load synthesis is realized, the overall synthesis yield of the synthetic chip is improved, and the method can be applied to the field of organic synthesis.

Description

technical field [0001] The invention relates to the field of synthetic biology, in particular to a nucleic acid synthesis-oriented microreactor array chip and a preparation method thereof. Background technique [0002] Synthetic biology uses the method of de novo synthesis to artificially synthesize genomes and artificially create new biological systems, which are disruptive innovative technologies that urgently need key breakthroughs in this century. Nucleic acid synthesis technology is a "stuck neck" technology in the development of synthetic biology, and the oligonucleotide synthesizer is its core equipment. Since the 1990s, the United States, Britain and other countries have carried out oligonucleotide synthesis on the basis of classical chemical methods. Instrument development. At present, column oligonucleotide synthesizers based on phosphoramidite chemical synthesis are widely used in the market. This column synthesizer achieves a yield of about micromoles per colum...

AI Technical Summary

Problems solved by technology

However, the single oligonucleotide synthesis concentration of the oligonucleotide synthesis chip is low, close to the femtomolar level, which cannot meet the needs of oligonucleotide assembly and artificial gene synthesis, and further research such as polymerase chain reaction (PCR) is still needed. and other amplification steps, thus resulting in a relatively redundant chip-based oligonucleotide synthesis step

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