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Source wastewater biotoxicity detection kit and use method thereof

A detection kit and biotoxicity technology, applied in biochemical equipment and methods, microbial measurement/inspection, biological material analysis, etc., can solve problems such as difficult observation, and achieve suitable tolerance, clear and stable color development Effect

Pending Publication Date: 2022-05-10
HANGZHOU XIUCHUAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the patent CN201910403966.2 discloses a differentiated management method for pharmaceutical wastewater based on MIC toxicity detection technology, using G, which accounts for the first abundance in activated sludge + bacteria and G - The standard strains of bacteria can judge the toxicity of wastewater by observing the growth of the two strains in wastewater with different dilutions, but the growth of the strains can only be reflected by the abundance of the strains in the wastewater, which is difficult to observe intuitively

Method used

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  • Source wastewater biotoxicity detection kit and use method thereof
  • Source wastewater biotoxicity detection kit and use method thereof
  • Source wastewater biotoxicity detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the degree of color development of different strains

[0037] According to the data survey, the typical strain with escin hydrolase activity is Enterococcus. Enterococci are pathogenic bacteria and are not suitable for use. Enterococci belong to Lactobacillales in terms of microbial classification. It is speculated that the Lactobacillales may have similar biochemical characteristics, so the model strains are focused on Lactobacillales.

[0038] Select 8 strains of lactic acid bacteria from different sources, Enterococcus faecalis, and Escherichia coli as the test bacteria, and inoculate the bacterial powder of each strain into two kinds of chromogenic agent aescin, respectively, according to the inoculum amount of 1% (0.1g / L). In the nutrient medium, culture in a constant temperature shaking incubator at 30°C for 24 hours, observe the color development, and the results are shown in Table 1.

[0039] The formula of medium 1 is: tryptone 10g / L, yeast extr...

Embodiment 2

[0046] Embodiment 2: the influence of salinity on bacterial strain growth

[0047] Add different amounts of NaCl to the medium 1, inoculate the powder of each strain according to the inoculum amount of 1g / L, and place it in a constant temperature shaking incubator at 30°C for 24 hours to observe the growth of the strain, and quantitatively measure it with a spectrophotometer The turbidity of bacterial liquid under the wavelength 600nm, the results are shown in Table 2.

[0048] The impact of table 2 salinity on bacterial growth

[0049]

[0050] The model strain needs to meet the following requirements: within the conventional salinity range (≤3wt%), it can grow normally and develop color; that is, this series of technologies cannot characterize the toxicity within the conventional salinity range (≤3wt%).

[0051] According to Table 2, it can be seen that the addition of salinity will have a more obvious impact on the growth of bacterial species. In the range of 0-3wt% sal...

Embodiment 3

[0052] Example 3: Effects of organic matter and inorganic ions on color development

[0053] Add various substances in medium 2 that may affect the color development to medium 1, inoculate the powder of each strain according to the inoculum amount of 1g / L, and place it in a constant temperature shaking incubator at 30°C for 24 hours to observe the color development. The results are shown in Table 3.

[0054] The influence of each material of table 3 on bacterial strain color development

[0055]

[0056] According to Table 3, it can be seen that the addition of glucose will obviously inhibit color development, and the addition of sodium acetate is beneficial to color development, so the detection technology of the present invention needs to be alert to whether glucose and sodium acetate are contained in the water sample. Magnesium sulfate and manganese sulfate have almost no effect on 1# strain and 8# strain, so 1# and 8# strains can be used as mimic strains.

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Abstract

The invention relates to the technical field of sewage treatment, and discloses a source wastewater biotoxicity detection kit and a use method thereof. The kit comprises a Q1 reagent and an F1 reagent, the Q1 reagent is a culture medium containing aesculin and iron ions; the reagent F1 is prepared from lactobacillus plantarum CR3; the lactobacillus plantarum CR3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 15, 2021, and the preservation number is CGMCC NO.22011. The lactobacillus plantarum CR3 has been preserved in the China General Microbiological Culture Collection Center (CGMCC). According to the method, the lactobacillus plantarum CR3 is applied to source wastewater biotoxicity detection, the culture medium adaptive to the lactobacillus plantarum CR3 is adopted, the wastewater toxicity intensity can be visually reflected through the color development depth of a culture system, the color development is obvious and stable, and whether front-end wastewater can directly enter a rear-end biochemical treatment system or not can be conveniently judged.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a source wastewater biotoxicity detection kit and a use method thereof. Background technique [0002] With the rapid development of my country's industry, the types of pollutants in water are becoming more and more complex. Many special pollutants will inhibit or even destroy the biological treatment of wastewater when the concentration reaches a certain threshold, such as heavy metals, chloroaniline, cyanide, nitrobenzene, etc., only use COD, BOD, TN, TP, etc. Conventional water quality indicators cannot objectively and accurately evaluate the water quality of wastewater. Therefore, the classification and diversion based on conventional water quality indicators can no longer meet the current status of industrial wastewater treatment. Source classification and diversion based on the toxicity of wastewater are required. [0003] Bio-toxicity testing technology (Bio-toxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/045G01N2333/335
Inventor 张文武苏悦杨宇斯王开彬夏雨王伟
Owner HANGZHOU XIUCHUAN TECH CO LTD
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