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Preparation method and application of red light carbon dots and micelles

A technology of carbon dots and red light, which is applied in the field of preparation of carbon dots and micelles, can solve the problems of multiple cleanings, short emission wavelengths, complex purification steps, etc., and achieve the effects of avoiding interference, enhancing water dispersibility, and low cost

Active Publication Date: 2022-05-10
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these carbon dots still have problems such as short emission wavelength, need for multiple washings, non-specific LD targeting or complicated preparation / purification steps.

Method used

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  • Preparation method and application of red light carbon dots and micelles
  • Preparation method and application of red light carbon dots and micelles
  • Preparation method and application of red light carbon dots and micelles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation steps of the red light carbon dots in this embodiment are as follows:

[0028] (1) Weigh 1g of 3-aminophenylboronic acid solid powder and add it to a 10mL polytetrafluoroethylene lining, and put the lining into a matching autoclave;

[0029] (2) Reaction: the autoclave is sealed and reacted in an oven at 220° C. for 20 h, then taken out to room temperature and cooled naturally;

[0030] (3) Purification: The product was washed three times with deionized water to remove impurities, and then the obtained solid was placed in an oven at 60° C. and dried overnight to obtain the final product (ie, red light carbon dots), which was stored in a refrigerator at 4° C.

[0031] The red light carbon dots obtained by the above method are dispersed in ethanol solvent and the transmission electron microscope picture obtained is shown in figure 2 . Experimental results show that the particle size of the red carbon dots is 6.9nm. After the red carbon dots obtained by...

Embodiment 2

[0033] To test the imaging effect of the red light carbon dots prepared in Example 1 on the lipid droplets of A549 cells, the method is as follows:

[0034] (1) Cell culture: A549 cells were cultured at 5×10 4 Seed in a 96-well plate at a density of 1 / mL (100 μL) at 37°C, 5% CO 2 Cultivate in the environment for 24h;

[0035] (2) Cell staining: wash three times with phosphate buffered saline (PBS), add 100 μL PBS containing 20 μg / mL red light carbon dots and commercial lipid droplet fluorescent dye BODIPY 493 / 503 (1 μg / mL) and incubate for 15 min, wash with PBS cells 3 times;

[0036] (3) Confocal fluorescence microscopy imaging observation: Confocal fluorescence images were taken under the excitation of 552nm laser (red light carbon dots) and 488nm laser (BODIPY 493 / 503) using a laser confocal fluorescence microscope.

[0037] The results of confocal fluorescence imaging of cells after co-staining of red carbon dots and BODIPY 493 / 503 observed by the above method are as fo...

Embodiment 3

[0039] The preparation of micelles based on red light carbon dots in this embodiment, the specific steps are as follows:

[0040] (1) Preparation: Disperse 20mg of PF-127 or DPO and 2mg of red carbon dots in 0.5mL of dichloromethane, and dry with nitrogen;

[0041] (2) Purification: drying the sample obtained above in a vacuum drying oven at room temperature for more than 1 h;

[0042] (3) Resuspension: Add 1 mL of deionized water to each dried sample, and sonicate the resulting suspension at 25°C for 30 min to prepare a lipid droplet-specific targeting gel based on red light carbon dots. bundles (i.e. "PF-127 / Red Carbon Dots" and "DPO / Red Carbon Dots").

[0043] The transmission electron microscope pictures of lipid droplet-specific targeting micelles based on red light carbon dots obtained by the above method are shown in Figure 5 . The results show that the micelles based on red light carbon dots have good water dispersibility and uniform particle size. The particle size ...

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Abstract

The invention discloses a preparation method and application of red light carbon dots and micelles. The red light carbon dot is prepared from 3-aminophenylboronic acid through a solvent-free carbonization method. The carbon dots have the characteristics of simple synthesis process, long-wavelength fluorescence emission, good light stability, large Stokes shift, fluorescence emission independent of excitation wavelength and dependent on solvent polarity, good biological safety and the like, and high-quality living cell lipid droplet no-clean imaging can be realized. In addition, the carbon dots can also be used for monitoring the dynamic change of lipid droplets in living cells and the autophagy behavior of the lipid droplets.

Description

technical field [0001] The invention relates to a preparation method and application of carbon dots and micelles, in particular to a preparation method and application of red light carbon dots and micelles. Background technique [0002] Lipid droplets (LDs) are the main storage place for intracellular neutral lipids, which exist in almost all eukaryotic cells. The core of the lipid droplet is composed of neutral lipids, and there is a single layer of phospholipid molecules and various proteins outside the core. As a highly dynamic organelle for intracellular lipid storage and transport, lipid droplets can exchange materials with other organelles such as endoplasmic reticulum, Golgi apparatus, and mitochondria. Therefore, lipid droplets are associated with many physiological processes, such as lipid metabolism, protein degradation, membrane formation, energy storage, and signal transduction, etc. In addition, the formation of lipid droplets is also associated with many huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/6402C01P2004/04C01P2004/64Y02B20/00
Inventor 吴富根王梓豪佘聪
Owner SOUTHEAST UNIV
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