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Application of Prrc2b in preparation of medicine for treating demyelination disease

A technology of demyelination and myelination, applied in the field of biomedicine, which can solve the problems of few research results and so on

Active Publication Date: 2022-05-06
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the role of mRNA methylation modification in oligodendrocyte development has gradually become a research hotspot, but there are still relatively few research results on it. It is used as an intervention target to regulate oligodendrocyte differentiation, and then regulate Myelin formation, to achieve the purpose of treating demyelination-related diseases has important clinical value

Method used

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  • Application of Prrc2b in preparation of medicine for treating demyelination disease
  • Application of Prrc2b in preparation of medicine for treating demyelination disease
  • Application of Prrc2b in preparation of medicine for treating demyelination disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 m 6 A binding protein detection

[0054] 1. Main experimental materials

[0055] C57BL / 6J mice, HA-prrc2b transgenic mice (provided by Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences), Protein A / G magnetic beads (Bimake), Pierce TM Anti-HA-tag magnetic beads (Thermo Scientific), TRIzol (Invitrogen), BCA kit (Beiyuntian Biotechnology), RNasin (Promega), proteinase K (Roche), MNase (Biolabs).

[0056] 2. Experimental method

[0057] 1) In vitro RNA pull-down

[0058] Brain tissue of embryonic E16 mice (C57BL / 6J mice) was isolated, protein was extracted and BCA was quantified. Protein lysate pretreatment: incubate magnetic beads with lysate at 4°C for 1 h to remove non-specific binding; streptomycin-linked magnetic (ThermoScientific) pretreatment: mix magnetic beads with 0.2 mg / ml tRNA (Sigma) and 0.2 mg / ml BSA was incubated at 4°C for 1 h to block the non-specific sites on the magnetic beads. The pretreated protein lysate and...

Embodiment 2

[0070] Example 2 Physiological function research of Prrc2b

[0071] 1. Experimental materials

[0072] C57BL / 6J mice, Prrc2b conditional knockout mice, MBP antibody (Abcam), Olig2 antibody (Millipore), Pdgfrα antibody (BD Bioscience), Trizol (Invitrogen), BCA kit (Beyond Biotech), Black-GoldⅡ Myelin staining kit (Millipore), CC1 antibody (Millipore).

[0073] 2. Experimental method

[0074] 1) Breeding Prrc2b conditional knockout mice

[0075] Prrc2b f / f The mouse (constructed by Dr. Wang Fengchao, Beijing Institute of Life Sciences, donated by Dr. Zhang Jinhua, Beijing Jiaotong University) and the Olig2-Cre mouse (gifted by Prof. Xiao Bo, Sichuan University, Stock No. 011103, JacksonLaboratory) were crossed to obtain Prrc2b f / + ; Olig2-Cre mice. Prrc2b f / f Mice and Prrc2b f / +; Olig2-Cre mice were crossed to obtain offspring Prrc2b f / f , Prrc2b f / + ; Olig2-Cre and Prrc2b knockout of the Prrc2b gene in the Olig2-expressing oligodendrocyte lineage f / f ; Olig2-Cre.

[...

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Abstract

The invention discloses an application of Prrc2b in preparation of a medicine for treating a demyelination disease. Experiments prove that Prrc2b is a novel m6A binding protein and regulates and controls the oligodendrocyte fate decision, and Prrc2b gene knockout can cause insufficient myelination or demyelination, so that Prrc2b can be used as a drug target for treating the demyelination disease. In addition, the invention also discloses an application of Prrc2b in construction of a myelin injury or demyelination animal model.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of Prrc2b in the preparation of medicines for treating demyelinating diseases. Background technique [0002] Myelin is a layer of myelin protein wrapped around the nerve fibers, which can wrap the axons to insulate the axons from each other and avoid interference; the Ranvier knot formed between the myelin sheaths can make the action potential "jump" on the axons Conduction" to speed up signal transmission; in addition, myelin wrapping around axons can also play a protective role. [0003] Can help axon regeneration in case of axon injury. When the myelin sheath is damaged or underdeveloped, it will cause some myelin sheath-related diseases, such as multiple sclerosis, optic neuromyelitis, etc., and studies have found that a variety of neurological diseases are accompanied by myelin sheath loss, such as Alzheimer's disease, depression syndrome and brain trauma. The cell...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K45/00A61P25/00G01N33/53C12Q1/6869
CPCA01K67/0276G01N33/5308C12Q1/6869A61K45/00A61P25/00A01K2217/15A01K2227/105A01K2267/03C12Q2535/122C12Q2537/164
Inventor 袁增强王树坤张莹李硕硕孔祥熙吴荣
Owner ACADEMY OF MILITARY MEDICAL SCI
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