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Ultralow-temperature preservation solution and preservation method for liver

An ultra-low temperature preservation and low temperature preservation technology, applied in the field of clinical medicine liver transplantation, can solve the problems of difficult to form a unified plan, 8-10 hours, clinical application limitations, etc., to prolong the effective preservation time, improve liver function, improve Save the effect of the effect

Active Publication Date: 2022-05-03
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the preservation of donor livers before transplantation, static cold preservation is still the most common method of liver preservation, but the disadvantage is that the clinically effective preservation time is only 8-10 hours, Forcing clinicians to perform liver transplants within 8 hours of donor liver preservation
Extracorporeal mechanical perfusion of the liver is a newly developed technology for liver preservation. Although this technology can improve the quality of the donor liver and prolong the effective preservation time of the organ for 24 hours, it is complicated, expensive, and difficult to transport. Very limited in clinical application
[0005] So far, there are few successful reports on cryopreservation of the liver, except for the reports of individual teams. The relevant technology is still immature, and it is difficult to form a unified plan

Method used

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  • Ultralow-temperature preservation solution and preservation method for liver
  • Ultralow-temperature preservation solution and preservation method for liver
  • Ultralow-temperature preservation solution and preservation method for liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Configure the cryopreservation solution, including the following steps:

[0051] Prepare 500mL loading solution according to the following dosage:

[0052]

[0053] Prepare 1000mL cryopreservation solution according to the following dosage:

[0054]

[0055] Prepare 500mL resuscitation solution according to the following dosage:

[0056]

[0057]

[0058] Use the above three solutions of loading solution, cryopreservation solution and resuscitation solution to sequentially treat the rat liver, and realize the cryopreservation treatment of the rat liver. The specific steps are as follows:

[0059] (1) Loading treatment: After the donor rat liver is obtained, use the isolated rat liver perfusion system to use the loading solution (150 mL) as the perfusion solution, and circulate the donor liver through the portal vein for 50 minutes at room temperature at 18°C , so that the donor liver can fully absorb 3-OMG and Cilostamide, and then at the end of the mechan...

Embodiment 2

[0065] The loading solution, cryopreservation solution and resuscitation solution in this example are different from those in Example 1 above, see Table 1 below.

[0066] Rat livers were cryopreserved, and the specific steps were as follows:

[0067] (1) Loading treatment: After the donor rat liver is obtained, use the isolated rat liver perfusion system, use the loading solution (150 mL) as the perfusion solution, and circulate the donor liver through the portal vein for 60 minutes at room temperature of 19°C , so that the donor liver can fully absorb 3-OMG and Cilostamide, and then at the end of the mechanical perfusion, the temperature is lowered to 3.5°C at a rate of 0.9°C / min by means of a water bath. Typically set the flow rate at 8 mL / min during the first minute of perfusion, monitoring the pressure closely to ensure it does not exceed 10 cmH 2 O(1cmH 2 O=0.098kPa); then slowly increase the flow rate to 12mL / min in increments of 1mL / min, ensuring that the pressure doe...

Embodiment 3

[0073] The loading solution, cryopreservation solution and resuscitation solution in this example are different from those in Example 1 above, see Table 1 below.

[0074] Rat livers were cryopreserved, and the specific steps were as follows:

[0075] (1) Loading treatment: After the donor rat liver is obtained, use the isolated rat liver perfusion system, use the loading solution (150 mL) as the perfusion fluid, and circulate the donor liver through the portal vein for 60 minutes at room temperature of 21°C , so that the donor liver can fully absorb 3-OMG and Cilostamide, and then at the end of the mechanical perfusion, the temperature is lowered to 4°C at a rate of 1.0°C / min by means of a water bath. Typically set the flow rate at 8 mL / min during the first minute of perfusion, monitoring the pressure closely to ensure it does not exceed 10 cmH 2 O(1cmH2 O=0.098kPa); then slowly increase the flow rate to 12mL / min in increments of 1mL / min, ensuring that the pressure does not e...

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Abstract

The invention relates to the technical field of liver transplantation in clinical medicine, in particular to an ultralow-temperature preservation solution and a preservation method for liver. The ultralow-temperature preservation solution comprises a loading solution, a low-temperature preservation solution and a recovery solution, the loading solution is prepared from a Williams E culture medium, 3-O-methyl-D-glucopyranose and silolamide; the ultralow-temperature preservation solution comprises a UW solution, polyethylene glycol and silolamide; the resuscitation solution is prepared from a Williams E culture medium and silolamide. 3-O-methyl-D-glucopyranose, silolamide and polyethylene glycol are combined to treat the in-vitro liver, the in-vitro liver is protected, the in-vitro liver can be preserved at the ultralow temperature of minus 6 DEG C to minus 6 DEG C, the effective preservation time of the donor liver is effectively prolonged to 48-72 hours, the unfrozen in-vitro hepatocytes have good quality, and the unfrozen in-vitro hepatocytes are free of toxic and side effects. The problem of liver tissue damage caused by low temperature is obviously relieved, and the liver transplantation curative effect is improved.

Description

technical field [0001] The invention relates to the technical field of liver transplantation in clinical medicine, in particular to a liver cryopreservation solution and a preservation method. Background technique [0002] The liver is the largest digestive gland in the human body, the central station of material and energy metabolism in the body, and an essential organ to maintain life activities. Liver disease refers to lesions that occur in the liver, including hepatitis B, hepatitis A, hepatitis C, cirrhosis, fatty liver, liver cancer, alcoholic liver and other liver diseases. It is a common and extremely harmful disease. [0003] The three principles of liver disease treatment include anti-virus, improving immunity, and restoring liver function. The most effective treatment for end-stage liver disease is liver transplantation. The good curative effect of liver transplantation depends on the quality of the donor liver and the level of clinical technology. Due to the ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0284
Inventor 焦兴元李益虎张琪何睿黄颖滨邰强
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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