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Method and kit for the detection of 19 drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry

A technology of tandem mass spectrometry and liquid chromatography, applied in the field of detection of 19 kinds of drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry, can solve the problems of critical value of drugs, poisoning, etc. The effect of short time and high sensitivity

Active Publication Date: 2022-07-22
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Antipsychotics, anti-anxiety drugs, antidepressants, cardiac glycosides, antiepileptic drugs, platelet aggregation inhibitors, hypoglycemic drugs and other drugs will be taken by patients at the same time. Different drugs work for different diseases, and the blood concentration of the above drugs is required. Monitoring, individual drugs still have critical values, which will cause poisoning after exceeding a certain concentration

Method used

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  • Method and kit for the detection of 19 drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry
  • Method and kit for the detection of 19 drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry
  • Method and kit for the detection of 19 drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0131] 1. Clopidogrel: Accurately weigh 1.56 mg of standard clopidogrel hydrogen sulfate, put it in a 2 mL cryopreservation tube, add 2 mL of methanol to dissolve and dilute to volume to obtain a mother solution with a concentration of 597.8 μg / mL.

[0132] 2. Clopidogrel metabolites: Add 1 ml of methanol to the sample bottle containing 1 mg of clopidogrel active metabolite derivatives to dissolve to obtain a stock solution with a concentration of 1000 μg / mL.

[0133] 3. 1-(2-Pyrimidine)piperazine: Precisely weigh 5.78 mg of 1-(2-pyrimidine)piperazine standard into a 2 mL cryovial, add 2 mL of methanol to dissolve, and mix to obtain a concentration of 2832 μg / mL of mother liquor.

[0134] 4. Tandospirone: Accurately weigh 2.13 mg of standard tandospirone into a 2 mL cryovial, add 2 mL of methanol to dissolve, and mix to obtain a mother liquor with a concentration of 1044 μg / mL.

[0135] 5. Mianserin: The purchased standard is of known concentration, the volume is 1 mL, the s...

Embodiment 2

[0168] This example is used to illustrate a specific embodiment of the preparation of the internal standard working solution:

[0169] 1. Clopidogrel-D3: Accurately weigh 1.79 mg of standard clopidogrel-D3 sulfate, put it in a 2 mL cryopreservation tube, add 2 mL of methanol to dissolve, and obtain a mother solution with a concentration of 687.4 µg / mL.

[0170] 2. Clopidogrel active metabolite derivative-13C6: The purchased specification is 1 mg, and the actual specification is 1.059 mg. Directly add 1 mL of methanol to the sample bottle to dissolve, and obtain a mother solution with a concentration of 978.45 µg / mL.

[0171] 3. 1-(2-Pyrimidine)piperazine-D8: The standard specification is 2.5 mg, directly add 1 mL of methanol to the sample bottle to dissolve, transfer it to a 15 mL centrifuge tube, and then add 1 mL in 4 times. Dissolve in methanol and transfer the lysate to a 15 mL centrifuge tube, and mix to obtain a stock solution with a concentration of 500 μg / mL.

[0172]...

Embodiment 3

[0197] This example is used to illustrate a specific implementation of sample processing and detection:

[0198] 1. First, use a pipette to mix at least three different concentrations of standard working solution 10 μL, internal standard working solution 10 μL and methanol: water volume ratio of 1:1 mixed solution 80 μL in 1.5 mL centrifuge tubes. At least three standard solutions, the above standard solutions were vortexed and mixed for 30s at a rotational speed of 2000 rpm respectively, and then the above standard solutions were detected by liquid chromatography-mass spectrometry (specific parameters are the same as S3), and the above at least three standard solutions were obtained. Solution, 19 kinds of drugs and their metabolites and internal standard chromatograms, take the ratio of the peak area of ​​each substance of the above at least three standard solutions and the peak area of ​​the corresponding internal standard as the ordinate y of the standard curve, take the abo...

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Abstract

The invention belongs to the technical field of drug detection, and particularly relates to a method and a kit for detecting 19 kinds of drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry. Substances to be tested include sulpiride, penfluridol, mianserin, buspirone, tandospirone, hydroxyzine, diazepam, venlafaxine, moclobemide, imipramine, paroxetine, rebo Cetine, amitriptyline, sertraline, digoxin, clonazepam, clopidogrel, tolbutamide, glimepiride, 1-pyrimidine piperazine, desvenlafaxine, 6- Hydroxybuspirone, norimipramine, noramitriptyline, nordiazepam, clopidogrel metabolites; detection methods include: calibrating standard solutions, processing samples to be tested, using high performance liquid chromatography‑ Mass spectrometry detects the sample to be tested. The embodiment of the present invention can quickly and accurately measure the content, the sample processing method is simple and easy, and has high sensitivity and accurate quantification.

Description

technical field [0001] The invention belongs to the technical field of drug detection, in particular to a method and a kit for detecting 19 kinds of drugs and their metabolites in blood by liquid chromatography tandem mass spectrometry. Background technique [0002] Therapeutic drug monitoring (TDM) is a pharmacy clinical discipline that studies the mechanisms, techniques, methods and clinical standards of individualized drug treatment, and transforms the research results into clinical treatment to maximize rational drug use. By measuring drug exposure, pharmacological markers or pharmacodynamic indicators in patients, using quantitative pharmacological models, and taking the drug treatment window as the benchmark, an individualized dosing regimen suitable for patients is formulated. The core of which is individualized drug therapy. The clinical significance of TDM It lies in the ability to optimize drug treatment plans, improve drug efficacy, reduce toxic side effects, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/027G01N2030/045G01N2030/062
Inventor 贾永娟刘春冉刘杏立张杰倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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