Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Slow-transit constipation marking microorganism and application thereof

A technology of microorganisms and markers, applied in the direction of microorganisms, microorganism-based methods, and the determination/inspection of microorganisms, which can solve problems such as lack of bowel movements, bad living habits, abnormal hormone levels, etc.

Pending Publication Date: 2022-04-29
上海金翌生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now the medical pathology is not completely clear, it may be related to the regulation dysfunction of the enteric nervous system, central nervous system and autonomic nervous system, abnormal hormone levels, etc. At present, the consensus is long-term bad living habits, such as irregular living, too fine diet, Weight loss, dieting, and lack of exercise lead to insufficient intestinal stimulation, lack of defecation action, and feces stay in the intestinal cavity for too long, resulting in slow transit constipation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Slow-transit constipation marking microorganism and application thereof
  • Slow-transit constipation marking microorganism and application thereof
  • Slow-transit constipation marking microorganism and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Identification of biomarkers

[0065] In this example, the inventors studied 118 stool samples from the Tenth People's Hospital Affiliated to Tongji University, including 52 stool samples from patients with slow transit constipation and 66 stool samples from Chinese healthy controls. We divided the 118 samples into phase I and phase II, 90 samples in the first phase (including 40 patients with slow transit constipation and 50 normal subjects), 28 samples in the second phase (including 12 patients with slow transit constipation and 16 normal subjects). people). In Example 1, 118 samples were processed uniformly, including sample collection and DNA extraction, DNA library construction and sequencing, and microbial species abundance analysis. In Example 2, the screening of microbial species markers was carried out with 90 cases of Phase I data. In Example 3, the second-phase data of 28 cases were used to verify the microbial species markers.

[0066] 1. Sample...

Embodiment 2

[0092] Example 2 Screening for microbial species markers

[0093] In order to obtain markers of intestinal microbial species closely related to slow transit constipation disease, the inventors used the data of 90 cases of slow transit constipation patients group (40 cases) and normal control group (50 cases) to obtain intestinal microbial species markers. degree data, a study on the correlation with disease was done at the species level. Based on the species abundance table obtained in Example 1, the inventors set the standard as follows: (1) by combining the Wilcoxon rank sum test of Benjamini Hochberg's multiple test, the correlation p value of each species and slow transit constipation disease is obtained and q value; (2) Use a strict threshold (q value <0.04) to screen using the above parameters. The inventor obtained 7 intestinal microbial species closely related to slow transit constipation disease, and the data analysis is shown in Table 1, among which, there are 7 spe...

Embodiment 3

[0096] Verification of Example 3 Microbial Species Marker

[0097] In order to confirm the findings in Example 2, the inventors referred to the method described in Example 2 to determine the feces samples of 16 healthy people and 12 patients with slow transit constipation (28 cases of second-phase data) in the verification group. Show the abundance of 7 kinds of bacteria, and judge whether these 7 kinds of bacteria abundances of each sample species fall into the 95% confidence interval of the disease group or the healthy group determined in embodiment 2, determine the abundance of 7 kinds of bacteria The state of the individual corresponding to the sample whose abundance falls into the corresponding interval of the disease group is the patient with slow transit constipation, and the state of the individual corresponding to the sample whose abundance of the seven bacterial species falls within the corresponding interval of the healthy group is the patient with non-slow transit c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a slow transit constipation marking microorganism and application thereof, and further provides a kit according to the marking microorganism, which comprises a reagent suitable for detecting at least one strain in the marking microorganism, the invention relates to a method for preparing a microbial marker, which is characterized in that the microbial marker comprises the following strains: clostridium harzianum, Lachnospiraceae bacteria 3157 FAA CT1, a new species, Erysipastoris bacteria, Erysipastoris bacteria 2244A, Gordoniapastoris, Akkermansia muciniphila and clostridium symbiosum, and the microbial marker comprises the following strains: the clostridium harzianum, the Lachnospiraceae bacteria 3157 FAA CT1, the new species, the Erysipastoris bacteria 2244A, the Gordoniapastoris bacteria 2244A, the Akkermansia muciniphila and the clostridium symbiosum. The abundance of the microorganism provided by the invention is remarkably different in healthy people and patients with slow transit constipation, and the microorganism can be used as a marker for detecting and / or treating slow transit constipation.

Description

technical field [0001] The present invention relates to the field of biotechnology. Specifically, the present invention relates to slow transit constipation marker microorganisms and applications thereof. More specifically, the present invention relates to a kit, the use of the reagent in the preparation of the kit, and the method for preventing or treating slow transit Drug-marker microorganisms or food-marker microorganisms for constipation, a method for determining whether an individual suffers from slow transit constipation, a device for determining whether an individual suffers from slow transit constipation, a device, and a method for screening drugs. Background technique [0002] Slow transit constipation (Slow Transit Constipation, STC) is a kind of constipation with relatively slow transit. Due to the dysfunction of the large intestine and the abnormal conduction, it leads to prolonged defecation cycle and difficult defecation. It belongs to chronic, primary, functio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6883C12R1/145C12R1/01
CPCC12Q1/689C12Q1/6883
Inventor 郑智俊秦楠刘强
Owner 上海金翌生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com