Ankylosing spondylitis microbial marker
Ankylosing spondylitis, microbiological technology, applied in the direction of microbiological determination/testing, biochemical equipment and methods, anti-inflammatory agents, etc., can solve problems such as intestinal microbial markers in patients without ankylosing spondylitis
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Embodiment 1
[0073] In this example, the inventors carried out an association analysis study of the whole intestinal flora microbes from 73 ankylosing spondylitis patients and 83 healthy controls' stool samples to describe the characteristics of the fecal microbial community and functional components. In general, the inventor downloaded about 428.09Gb of high-quality sequencing data (LC healthy people) and 294Gb of high-quality sequencing data obtained from experimental sequencing to construct an ankylosing spondylitis reference gene set, and compared with the downloaded LC gene set, IGC Gene sets build a more complete gene set. Metagenome analysis showed that 17 microbial species were closely related to AS disease, 7 of which were enriched in the gut microbes of healthy people, and 10 of which were enriched in the gut microbes of AS patients.
[0074] 1. Acquisition of sequencing data
[0075] 1.1 Sample collection and DNA extraction
[0076] Patients with ankylosing spondylitis came fr...
Embodiment 2
[0103] In order to confirm that the analysis results in Example 1 can be used as an ankylosing spondylitis species marker, further compare the species abundance of 31 healthy people and 24 ankylosing spondylitis patients in the verification group, and mark the final microbial species Items are screened based on validation. The acquisition and processing of the sequencing data of the verification population is carried out with reference to Example 1.
[0104] The verification results are as follows: Among the 14 species enriched in the healthy population, 7 of them obtained high-quality verification in the verification set (p_values<0.05), and the p-value and q-value of the verification of the microbial species markers enriched in healthy people As shown in Table 2 below. The microbial species were Lachnospiraceae bacterium 6_1_63FAA, Fusobacterium mortiferum, [Bacteroides] pectinophilus, Enterobacter cloacae, Eubacterium eligens, Lachnospiraceae (Lachnospiraceae bacterium 8_...
Embodiment 3
[0115] Forty-five stool samples were used to test the individual status of the sample source.
[0116] Determine actinomycetes (Actinomyces sp.ICM47), Bifidobacterium breve (Bifidobacterium breve), Bifidobacterium (Bifidobacterium sp.12_1_47BFAA), Bifidobacterium longum (Bifidobacterium sp. longum), Collinsella aerofaciens, Bifidobacterium bifidum and Bifidobacterium pseudocatenulatum, to determine whether the abundance of these seven strains in each sample falls into the Each is in the 95% confidence interval of the abundance of the disease control group or the healthy control group, and it is determined that the abundance of these seven strains falls into the corresponding interval of the disease group, and the individual corresponding to the sample is an ankylosing spondylitis patient , it is determined that the state of the individual corresponding to the sample whose abundance of the seven strains all falls within the corresponding interval of the healthy group is a non-a...
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