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Trichoderma asperellum and application thereof

A technology of Trichoderma aculeatus and fungi, applied in the field of Trichoderma aculeatus, can solve the problems of pesticide residues, rampant pathogens, enhanced drug resistance of pathogens, etc., and achieve the effect of high safety

Active Publication Date: 2022-04-29
广州市土根旺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the continuous and large-scale use of chemical pesticides has caused serious "3R" problems, that is, the increased resistance of pathogenic bacteria, pesticide residues in the soil or the environment, and the re-emergence of pathogens

Method used

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  • Trichoderma asperellum and application thereof
  • Trichoderma asperellum and application thereof
  • Trichoderma asperellum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Isolation and identification of Trichoderma strain YN4

[0068] 1. Sample collection

[0069] Tea leaves were collected from Honghe Prefecture, Yunnan, and returned to the laboratory for tissue isolation. First, clean the surface of the leaves, and select intact leaves without disease spots for separation. For the method of tissue isolation, please refer to "Plant Pathology Research Methods".

[0070] 2. Morphological identification

[0071] From the colonies isolated from the tea tree leaf tissue, the newly grown colonies were picked with sterile toothpicks and cultured on a new PDA plate, and cultured in a 28°C incubator for 2-3 days. Observe the colony morphology and color of the strain. Take an appropriate amount of bacteria with a sterile dissecting needle and observe it under an optical microscope, and take pictures of hyphae, conidia, etc. According to the morphological characteristics of the strain, it was preliminarily identified as Trichoderma, ...

Embodiment 2

[0080] Example 2 Inhibitory effect of Trichoderma strain YN4 on various pathogenic bacteria

[0081] 1. Experimental method

[0082] The antagonistic effect of the strain T. asperellum YN4 identified in Example 1 against 12 phytopathogenic fungi was determined by the confrontation method.

[0083] Tested phytopathogenic fungi: F. oxysporum f. sp. glycines, F. oxysporum f. sp. cube, F. oxysporum f. sp. .solani), F. falciforme, C. siamense, C. gloeosporioides, C. truncatum, S. sclerotiorum rolfsii), N. sphaerica, R. solani, P. oryzae, and B. cinerea. The above plant pathogenic fungal strains are all preserved by our laboratory.

[0084] Specific practices: the strains T. asperellum YN4 and the test plant pathogenic fungi identified in Example 1 were grown in PDA plates for 3 to 5 days, respectively, using a hole punch with a diameter of 5 mm to punch holes at the edge of the colony to obtain bacteria. cake. In a PDA plate with a diameter of about 9cm, two places in diameter 2...

Embodiment 3

[0095] The antibacterial effect of embodiment 3 Trichoderma strain YN4 volatile organic matter

[0096] 1. Antibacterial effect of volatile organic compounds of Trichoderma strains

[0097] 1. Experimental method

[0098] Use a sterilized hole puncher with a diameter of 5mm in a constant temperature incubator at 28°C for 2 to 3 days to cultivate the bacterial colony edge of the strain Trichoderma asperellum (T. asperellum) YN4 identified in Example 1. The center of the petri dish with a 9 cm medium containing PDA was cultured at 28° C. until the colony diameter reached 5 cm, and a sterilized double cellophane with a diameter of 10 cm was covered above. Four kinds of plant pathogenic fungi (Siamese anthracnose, Fusarium spp., Fusarium oxysporum Cuban-specialized type and Fusarium rot) were made into 5 mm diameter cakes and inoculated into petri dishes containing PDA medium middle. The petri dish inoculated with the strain T. asperellum (T. asperellum) YN4 (covered with steri...

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Abstract

The invention discloses an endophytic trichoderma asperellum YN4 and application thereof, the endophytic trichoderma asperellum YN4 is preserved in Guangdong Microbial Culture Collection Center on October 22, 2021, and the preservation number is GDMCC NO: 62007. The strain can significantly inhibit the growth of fusarium oxysporum soybean specialized type, fusarium oxysporum f.sp.cubense specialized type, fusarium solani, fusarium oxysporum, colletotrichum siamensis, colletotrichum gloeosporioides, colletotrichum truncatum, sclerotium rolfsii, melanospora globosa, rhizoctonia solani, magnaporthe oryzae and botrytis cinerea; the composition can be used for efficiently preventing and treating botrytis cinerea, cabbage damping off, pepper anthracnose and soybean sheath blight; volatile organic compounds also have a biocontrol effect; the strain can also significantly inhibit germination of sclerotium in soil, and is expected to be used for preventing and treating sclerotiniose. The strain is derived from plant endophyte, is high in safety, can be used for development of microbial bactericides, can reduce or replace chemical bactericides, and provides powerful support for green prevention and control of crops.

Description

technical field [0001] The invention relates to the technical field of fungi prevention, in particular to a strain of Trichoderma aculeatus and its application. Background technique [0002] The occurrence of plant diseases causes a lot of economic losses every year. At present, the mainstay of plant disease control is chemical control. However, the continuous and large-scale use of chemical pesticides has caused serious "3R" problems, that is, the increased resistance of pathogens, pesticide residues in the soil or the environment, and the re-emergence of pathogens. This not only threatens human health, but also causes serious harm to the environment and non-target organisms. [0003] Biological control is one of the important technologies for the green prevention and control of crops. Biological control screening utilizes beneficial microorganisms and makes biological preparations to prevent and control the occurrence of plant diseases and insect pests, so as to achie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G13/00A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01G13/00A01N63/38
Inventor 舒灿伟张媛媛郑鹏刘小雪陈长明周而勋刘少群孙思常长青朱张生范昱黄智星
Owner 广州市土根旺生物科技有限公司
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