In-vitro rumen bionic system

Abstract

An in-vitro rumen bionic system comprises: a cellulose biomass pretreatment unit for pretreating cellulose biomass to obtain a matrix; the acidogenic fermentation unit is connected with the cellulose biomass pretreatment unit, inoculum rich in rumen functional bacteria is inoculated, the ruminant rumen anaerobic fermentation acidogenic environment is simulated, the matrix is acidified to obtain mixed liquid, and solid-liquid separation is performed; and the acid absorption unit is connected with the acid production fermentation unit and is used for absorbing and converting the short-chain fatty acid in the acidizing fluid obtained by solid-liquid separation. The method has the main advantages that 1, the physical and chemical environment in the rumen functional bacteria can be well simulated, effective retention of the rumen functional bacteria can be achieved, and long-term efficient and stable operation of an in-vitro bionic system is achieved; (2) the construction method of the system is simple, the implementation way of cellulose biomass energy is mild and efficient, and the operation and maintenance cost is low.

Description

technical field [0001] The invention belongs to the technical field of cellulose biomass resource utilization, and in particular relates to an in vitro rumen bionic system. Background technique [0002] In nature, the rumen of ruminants is a naturally formed and highly integrated natural biological reaction system that can efficiently hydrolyze cellulosic biomass. Among them, the rumen microbial flora and its secreted cellulosic biomass degrading enzymes, such as: lignin peroxidase, manganese peroxidase, laccase, xylanase, carboxymethyl cellulase, xylose Alcoholase, xylan esterase, β-glucosidase, endo-β-1,4-glucanase and cellobiohydrolase, etc. In the rumen of ruminants, it can quickly convert the refractory cellulosic biomass in nature into volatile short-chain fatty acids (VFA) under mild conditions, and then be absorbed by the rumen wall and transform into ATP to provide the required energy source for ruminants . [0003] With the development of global agriculture and ...

AI Technical Summary

Problems solved by technology

[0004] In view of the complexity of the current in vitro rumen bionic system and the difficulty of long-term efficient and stable operation, the present invention proposes an in vitro rumen bionic system, which is formed by coupling dynamic membrane bioreactors, rumen functional microorganisms, and methane-producing granular sludge to form acid-producing fermentation units. and acid absorption unit, respectively simulating the rumen fermentation process and rumen wall acid absorption function, effectively retaining rumen functional microorganisms and the extracellular cellulose biomass degrading enzymes secreted by them, and realizing the long-term efficient and stable operation of the in vitro rumen bionic system. The advantages of low investment and operation and simplicity

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