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Polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive as well as preparation method and application thereof

A technology of silk fibroin and polyethylene glycol, which is used in the preparation of biomedical materials and biomedical applications, can solve the problems of low adhesion strength on wet surfaces, obstacles to wide application, poor antibacterial performance, etc., and achieve excellent biocompatibility, Excellent antibacterial properties, the effect of increasing cohesion

Pending Publication Date: 2022-04-15
HARBIN ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing medical tissue adhesives such as cyanoacrylate glue and fibrin sealant have disadvantages such as low adhesion strength to wet surfaces, poor antibacterial performance, and high price, which hinder their wide application in clinical practice.

Method used

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  • Polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive as well as preparation method and application thereof
  • Polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive as well as preparation method and application thereof
  • Polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A. Weigh 2g of shredded silkworm cocoons, put them into a sodium carbonate solution with a mass fraction of 2%, bath ratio 1:50, temperature 100°C, time 30min, degumming 3 times. Wash the degummed silk fibroin repeatedly with deionized water, and dry it in an oven at 60°C. Weigh an appropriate amount of silk fibroin and dissolve it in LiBr solution, place it in a 60°C water bath for 6 hours, and shake it slightly every hour to make it dissolve evenly. Pour the dissolved silk fibroin solution into a dialysis bag (molecular weight cut-off 7000), dialyze with deionized water for 3 days, replace the deionized water every 8h, and obtain a silk fibroin solution with a concentration of 40mg / ml.

[0029] B. Add carboxyl activator (0.16g NHS, 0.12g EDC) to 5ml silk fibroin solution, keep stirring for 30min under water bath conditions of 40°C, then add 2ml polyethylene glycol (20000Da ) solution continued to stir for 30min to obtain a mixed solution.

[0030] C. Weigh 10g of ta...

Embodiment 2

[0032] A. Weigh 2 g of shredded cocoons, put them into sodium bicarbonate with a mass fraction of 4%, bath ratio 1:50, temperature 100°C, time 30 min, degumming 3 times. Wash the degummed silk fibroin repeatedly with deionized water, and dry it in an oven at 60°C. Place an appropriate amount of dried silk fibroin in a ternary system solution with a molar ratio of CaCl2 / C2H5OH / H2O of 1:2:8, place it in a water bath at 60°C for 3 hours, and shake it slightly every hour to dissolve it uniform. Pour the dissolved silk fibroin solution into a dialysis bag (molecular weight cut-off 70000), dialyze with deionized water for 3 days, and change the deionized water every 8 hours to obtain a silk fibroin solution with a concentration of 40mg / ml.

[0033] B. Add carboxyl activator (0.16g NHS, 0.12g EDC) in 5ml silk fibroin solution, under keeping 40 ℃ of water bath conditions, stir 30min, then add 1.2ml concentration and be the polyethylene glycol of 0.1g / ml ( 20000Da) solution was stirr...

Embodiment 3

[0036] (1) Under the joint action of polyethylene glycol and polyphenolic compounds, silk fibroin rapidly precipitates from the solution, and forms the first layer of network structure with polyphenolic compounds under the action of hydrophobic-hydrogen bonds, while polyethylene glycol and polyphenolic compounds Polyphenolic compounds form the second layer of network structure through strong hydrogen bonds. At the same time, silk fibroin undergoes self-crosslinking under the action of carboxyl activators, which increases the cohesive force of the adhesive, thereby obtaining a multi-layer adhesive with adhesive properties. Phenol-Silk Fibroin-Polyethylene Glycol Medical Tissue Adhesive.

[0037] (2) The specific preparation steps of a polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive are as follows:

[0038] A. Preparation of silk fibroin solution: under 100°C water bath heating condition, degumming process is carried out to silk with weak lye; after drying, dis...

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Abstract

According to the polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive, the preparation method and the application, under the combined action of polyethylene glycol and a polyphenol compound, silk fibroin is rapidly separated out from a solution and forms a first-layer network structure with the polyphenol compound under the hydrophobic-hydrogen-bond action; meanwhile, a second-layer network structure is formed by the polyethylene glycol and the polyphenol compound through strong hydrogen-bond interaction, meanwhile, silk fibroin is subjected to self-crosslinking under the action of the carboxyl activating agent, cohesion of the adhesive is increased, and therefore the polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive with the adhesion performance is obtained. The raw materials and the preparation process of the medical tissue adhesive are non-toxic and harmless, the product is non-irritant to a human body and has excellent biocompatibility, the preparation method is simple, and technological parameters are easy to control.

Description

technical field [0001] The invention belongs to the field of biomedical material preparation and biomedical application, and specifically relates to a polyphenol-silk fibroin-polyethylene glycol medical tissue adhesive with high bonding strength and excellent biological functionality, a preparation method and its application . Background technique [0002] Millions of wounds caused by trauma or surgery each year must be closed rapidly in the first place, current wound closure techniques mainly use invasive techniques such as sutures, staples or clips, which often results in secondary tissue damage , microbial infection, and unsightly scarring after healing. Therefore, in recent years, doctors have increasingly turned to non-invasive wound closure methods, such as the use of medical tissue adhesives. The adhesive is easy to apply and quickly bonds surrounding tissue, preventing leakage of fluid through the anastomotic site and avoiding additional trauma to wound healing. H...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/04A61L24/00A61L15/20A61L15/26A61L15/32A61L15/42A61L15/44
Inventor 鲁玺丽李一波孟鑫鑫贺乔柏
Owner HARBIN ENG UNIV
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