Marker and probe composition for screening gastric cancer and application of marker and probe composition

A marker and composition technology, applied in the biological field, can solve problems such as the lack of non-invasive diagnostic methods, achieve high sensitivity and accuracy, and reduce harm

Pending Publication Date: 2022-04-12
BIOCHAIN BEIJING SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Early detection is the key to improving patient survival and cure rat

Method used

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  • Marker and probe composition for screening gastric cancer and application of marker and probe composition
  • Marker and probe composition for screening gastric cancer and application of marker and probe composition
  • Marker and probe composition for screening gastric cancer and application of marker and probe composition

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preparation example Construction

[0370] The preparation of the chip is mainly based on a glass sheet or a silicon sheet as a carrier, and oligonucleotide fragments or cDNAs are arranged on the carrier in sequence as probes by means of in situ synthesis and micro-array.

[0371] The chip of the present invention is based on the signal detection of DNA sequence hybridization after bisulfite treatment. Bisulfite treatment is to change unmethylated cytosine into uracil, while methylated cytosine remains unchanged. Change, then convert uracil to thymine, and finally carry out chip hybridization; finally judge the type of base added according to the fluorescent color, and then determine whether the site is methylated.

[0372] The present invention provides a method for gastric cancer screening, which comprises:

[0373] detect the methylation level of the marker, and

[0374] The risk of the subject suffering from gastric cancer is judged based on the methylation level, and the marker is selected from one of the ...

Embodiment 1

[0378] Example 1 Screening markers

[0379] 1) Sample collection: Download the 450k methylation microarray cancer tissue data in TCGA, involving a total of 7,769 cancer tissue samples of 26 types of tumors, including adrenocortical carcinoma (80), bladder urothelial carcinoma (409), acute myeloid Leukemia (140), brain low-grade glioma (654), breast cancer (740), cervical cancer (286), colorectal cancer (348), esophageal cancer (183), uveal melanoma (80), head and neck squamous Stem cell carcinoma (527), kidney cancer (660), liver cancer (377), lung adenocarcinoma (425), lung squamous cell carcinoma (372), diffuse large B-cell lymphoma (29), ovarian serous cystadenocarcinoma ( 10), pancreatic cancer (184), mesothelioma (116), prostate cancer (488), skin melanoma (104), sarcoma (117), gastric cancer (397), testicular cancer (134), thymus cancer (94) , thyroid cancer (506), endometrial cancer (309). For healthy people, Boercheng collected the plasma of 38 healthy people, and th...

Embodiment 2

[0551] 6 cases of human samples (S1-3 are samples from healthy people, S4-6 are samples from patients with gastric cancer), using the methylation marker detection method of the application, collecting peripheral blood according to the method in Example 1; building a library, and passing Illumina Platform sequencing; the sequencing data is analyzed through the above-mentioned bioinformatics process to obtain the methylation level of each marker. According to the threshold of each marker, the patient’s disease condition is predicted. If it exceeds the threshold, it is a cancer sample; if it is low The threshold is a healthy sample, and the specific results are shown in Table 19:

[0552] Among the interpretation results, 0 represents the classification as normal, that is, healthy; 1 represents the classification as abnormal, that is, tumor.

[0553] Table 19 Methylation values ​​and interpretation results of samples

[0554]

[0555]

[0556] To sum up, the inventors of t...

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Abstract

The invention discloses a marker and a probe composition for gastric cancer screening and application of the marker and the probe composition. The marker is selected from any one of 47 markers. According to the present invention, with the application of the marker, the methylation state of the gene can be sensitively and specifically detected so as to be used for the detection of the peripheral blood free DNA, and the composition is used for the screening of the asymptomatic population in the non-invasive manner so as to reduce the harm caused by the invasive detection; the composition has higher sensitivity and accuracy, and can realize real-time monitoring.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker for gastric cancer screening, a probe composition and applications thereof. Background technique [0002] Gastric cancer is one of the most common malignant tumors of the digestive system. Its pathogenesis is mainly related to Helicobacter pylori infection, diet, environment and genetic factors. It is one of the most common and deadly cancers in the world, especially in the elderly. In general, early detection of gastric cancer is crucial to increasing the chances of successful treatment and prolonging the patient's lifespan. The 5-year survival rate of early gastric cancer can reach more than 95%. However, early gastric cancer is difficult to detect due to its indistinct symptoms, and some patients may have reached an advanced stage when first diagnosed. Early detection is the key to improving patient survival and cure rates, but accurate non-invasive diagnostic methods ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
Inventor 王小奇杨亚东李永君吴宁宁郭媛媛关晋霞
Owner BIOCHAIN BEIJING SCI & TECH
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